应用化学

The significance and application of reinforcement of conventional polymers by POSS are reviewed.

综述了多面体低聚倍半硅氧烷改性传统聚合物的作用及其应用；介绍了POSS的纳米级笼形结构、良好的热力学和化学稳定性及耐热、阻燃等以及溶胶-凝胶法合成POSS的优缺点。

Open-field test,step-down avoidance test and immunohistochemstry staining were employedto study the effects of the extract of the fruit of Hyperieum perforatum on praxiology and cerebral pathology in the rat models.

制作大鼠慢性应激抑郁模型，应用敞箱法、跳台法、免疫组织化学染色研究贯叶连翘提取物对抑郁大鼠行为学、脑病理学的影响。

The applications of mechanochemical effects of polymer solids during pulverization in self-compatibilization of immiscible polymer blends, effective mixing of polymers with unmatched viscosities, self-plasticization of polymeric resins, property tailoring between polymer components as well as the recovery of single and commingled postconsumer or preconsumer polymeric wastes were reviewed.

综述了聚合物固体粉碎过程中产生的力化学效应在不相容聚合物混合物增容、高分子树脂的自增塑、粘度不匹配聚合物体系的有效混合、高分子废弃物循环回收利用等方面的应用。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

The current antifungal agents used in clinics usually have severe side-effects, it is necessary to seek for new antifungal agents with known or new mechanisms. China has abundant resources in traditional Chinese medicine, therefore exploition of new antifungal agents therefrom is a feasible way.We have screened antifungal activities of 14 ethanolic extracts from traditional Chinese medicines and 21 natural products by using Saccharomyces cerevisiae GL7 and Prototheca wickerhamii as the model microoranganisms. Among the TCMs tested, Cortex Phellodendri, Flos Caryophylli, Fructus Mume, Rhizoma Coptidis and Radix Sophorae tonkinensis showed high antifungal activity. This study layed certain foundation for the development of new antifungal agents.

为了开发中药资源，从传统中药中寻找有抗真菌活性的天然药物并阐明其作用机理以利于临床应用，论文对14种生药乙醇提取物及21种生药化学成分利用啤酒酵母突变型GL7（Saccharomyces cerevisiae GL7）和威克海姆原藻作为模式菌种进行了抗真菌活性筛选，其中黄柏、丁香、乌梅、黄连和山豆根等有很好的抑菌活性，为进一步研究开发成新的抗真菌药奠定了基础。

Pseudomembranous colitis also demonstrates marked wall thickening and, occasionally, skip areas but is associated with broad-spectrum antibiotic treatment or chemotherapy.

伪膜性肠炎也表现为肠壁显著增厚，偶尔会有跳跃征，这跟广谱抗生素的应用及化学治疗有关系

MethodsPrimary nasal pterygial tissues(15 eyes) and normal human conjunctival tissues(8 eyes) were determined by immunohistochemical staining, using monoclonal and polyconal antibodies specifically against MMP-3, LN and col Ⅲ. The intensity of reaction on individual sections was semiquantitatively graded.

方法应用S-P免疫组织化学染色方法检测15例翼状胬肉组织及8例正常球结膜组织中MMP-3、LN、col Ⅲ的免疫学定位及表达情况，用Masson及VG染色法检测各标本中胶原纤维及弹力纤维的分布情况。

Methods Immunohistochemistry method was used to stain the substance P in pulpal tissue which could directly reflect the activation of nerve by stimuli.

应用免疫组织化学染色的方法，对树脂充填深龋洞后不同时期P物质表达变化进行观察研究。

The immunohistochemical method was used to detect Fos expression in Central Nerve System induced by pulpitis pain.

应用免疫组织化学方法检测牙髓炎疼痛诱发中枢神经系统（central nerve system， CNS）c-fos的表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。