应用化学

Methods: The expressions of CGRP and VEGF in the wound tissue were detected by immunohistochmisty technique and MIAS image analysis system.

应用免疫组织化学方法、图像分析技术动态检测大鼠创伤组织中CGRP，VEGF的表达。

The application of the microchemical method to the sample preparation accelerates determination.

应用微量化学法技术处理样品，提高了分析速度。样品用乙腈提取，提取液过C18微柱净化。

After 24 hours, all rats were anesthetized again and their left common carotid arteries were cannulated to facilitate blood withdrawal for serum sample. The contents of tumor necrosis factor-a and interleukin-6 (IL-6) in serum were determined before and after experiment by ELISA; the activity of superoxide dismutase and content of malondialdehyde were determined by xanthinoxidanse method and modified TBA microdetermination. The content of NO2(superscript -)/NO3(superscript -), and activity of nitric oxide synthase and inducible NOS were determined by nitrate reductase method, and chemical chromogenic reaction, respectively; the immunoglobulin G, IgA, IgM and complement 3 (C3) and C4 were determined by immunoprecipitation method before and after experiment.

实验评估：手术创伤后24h进行采用酶联免疫吸附法测定实验前后血清肿瘤坏死因子α、白细胞介素6含量；应用黄嘌呤氧化酶法测定超氧化物歧化酶活性、改良TBA微量法测定丙二醛水平；以硝酸还原酶法、化学显色法分别检测NO2/NO3浓度及一氧化氮合酶、诱导型一氧化氮合酶活胜；以免疫比浊法经全自动生化分析仪检测血清免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。

XRD, 29Si, 27AI MAS NMR, the adsorption of nitrogen at low temperature,129Xe NMR, XPS, IR-TPD, NH3-TPD, the high vacuum volumetric analysis and pulse flow microreaction were used to study the difference in structure and property between the US-SSY zeolites obtained by quick and slow cooling after treating SSY zeolite with steam at high temperature.

应用X射线粉末衍射、29Si、27Al魔角旋转核磁共振、低温氮吸附、129Xe核磁共振、X射线光电子能谱、程序升温脱附和脉冲微反等多种物理、化学手段，详细考察了经高温水蒸汽处理后的超稳Y分子筛在急冷和自冷过程中产生的结构和性能差异，结果表明，冷却速率是制备分子筛必要的控制因素

The cellular structure, chemical characterization of foliar endodermis and element cellular distribution were investigated in Pinus bungeana leaves by the fluorescence microscope, Fourier transform infrared microspectroscopy and energy-dispersive X-ray analysis.

应用荧光显微技术、傅里叶变换显微红外光谱分析、扫描电镜及X-射线能谱微区分析等手段，对白皮松子叶、初生叶及2 a生针叶内皮层细胞径向壁的显微结构特征、化学成分，以及在叶子横切面上Na和Cl的微区分布进行分析。

The structure of three kinds of milled wood lignin and its chemical products was studied by FTIR and XPS.

应用傅里叶转换红外光谱和X射线光电子能谱，研究了3种提纯方法得到的竹木质素及其化学反应产物的化学结构特性。

To observe the influence of tumor necrosis factora on differentiation of rat mesencephalic neural stem cells,the numbers of neurons,astrocytes and oligodendrocytes generated from NSCs were analyzed after differentiation for 3 days by using i m munocytoche mistry technique.

为观察肿瘤坏死因子对神经干细胞分化的影响，本研究应用体外扩增的新生大鼠中脑NSCs，使用免疫组织化学技术，观察了肿瘤坏死因子一。对NSC、分化及其后代细胞的影响。

The kinetic mechanism of the synthesis of hydroxyapatite from monocalcium phosphate and calcium oxide in vibratory mill was studied by using mechanochemical principle, and a corresponding kinetic model was established.

应用机械化学原理，研究了在振动磨样机中由磷酸二氢钙和氢氧化钙合成羟基磷灰石过程的动力学机制，并建立了相应的动力学模型。

Methods Immunohistochemical and morphometrical analysis methods were performed on vital skin wounds. Results In normal and injury mouse skin IL-10 immunoreactivity was observed in epidermal cells.

方法应用免疫组织化学技术和形态计量法，对小鼠不同时程皮肤切创组织中IL-10不同表达部位（表皮细胞及表皮下组织的浸润细胞）的变化进行研究。

The macrocyclic polyamines are a kind of excellent ligands and have a substantially high binding affinity with transition metal ions. Macrocyclic polyamine metal complexes as enzyme models are widely used in chemical biology, medicine and gene therapy. 1, 4, 7, 10-tetrazacyclododecane is a representative macrocyclic polyamines compound and shows high binding affinity with transition metal ions, such as Zn, Cu and Co. Their metal complexes especially multinuclear complexes were widely used in DNA, RNA recognition.

大环多胺化合物是一类性质独特的优秀配体，具有良好的金属配位能力，其金属配合物作为人工酶模型广泛应用于核酸结构及功能研究、基因表达与调控、抗癌药物的研制等生物、化学和医学领域。1，4，7，10-四氮杂十二烷是典型的大环多胺化合物，对过渡金属离子Zn， Cu and Co有良好的配位能力，这些金属配合物特别是多核金属配合物被广泛应用于DNA识别中。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。