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In the present study changes of NOS positive cells after injection of LPS and apoptotic ependymal cells, the relationship between expression of immediate early gene c-fos and astrocytes, and comparison of changes between SFO and rest regions of the brain in different time-points were investigated with NADPH-d histochemistry, apoptosis in SITU detection of TUNEL, TEM and SEM, fluorescein double labeling of immunohistochemistry, and laser scanning confocal microscope.

为了证实穹窿下器在脑内免疫调节系统中的作用,在本研究当中,应用了NADPH刁的组化方法、TUNEL末端标记法、扫描及透射电镜方法、免疫组织化学的荧光双标法及激光扫描共聚焦显微镜技术,来研究在腹腔内给予大肠杆菌内毒素后,脑内的一氧化氮合酶细胞的变化、室管膜细胞的凋亡、早期即刻基因(immediate early genes沁-fos的表达与星形胶质细胞之间的关系,以及穹窿下器与其它脑区相比较时的特异性改变。

Spinal cord injury models were made in 60 wistar rats and the dynamic expression of bromodeoxyuridine and polysialylated ependymal cells adhesion molecule were determined by immuno-histochemisty.

方法]Wistar大鼠60只,制作大鼠脊髓全横断损伤模型,根据伤后不同时间点分组,应用免疫组织化学方法动态检测脊髓内5-溴脱氧尿嘧啶、多唾液酸神经细胞黏附分子的表达。

The models of EIOP were established by obstructing episcleral veins, using immunohistochemistry stain for PEDF expression in retina and evaluate the rule of the change.

应用巩膜浅层静脉烧烙法制作慢性高眼压(elevated intraocular pressure, EIOP)模型,用免疫组织化学染色法观察视网膜各层结构PEDF的表达和变化规律。

INTERVENTIONS: Embryonal 14-day SD retina was transplantedin-between the epithalamus and hypothalamus of the midbrain in P1 rat and the right eye of the P1 rat was removed simultaneously. The development time of TP on the 9th day after operation was corresponding to the normal retina on the 1st day after born, which was then recorded as TP1, and so on. The development and differentiation was shown by histochemical method.

干预:用胚14 d SD大鼠视网膜移植至P1大鼠中脑左侧上、下丘间,同时摘除P1鼠右眼,术后第9天移植视网膜的发育时间与生后第1天正常视网膜相当,记为TP1,余类推,应用组织化学方法显示神经元的发育和分化。

By using multiple ethological tests, immunohistochemistry and Thioflavin-S staining, we investigated neuroprotective effect and mechanism of HNG on ethology and Aβexpression in APP/PS-1 transgenic mice in vivo.

以转基因AD动物模型(APP/PS-1小鼠)为研究对象,应用多种行为学研究方法、免疫组织化学方法和荧光染色方法,探讨HNG对于AD转基因小鼠行为学障碍的治疗作用及其相关机制。

Some exercisable measures are probed into for training innovative thinking ability by application of induction and deduction in the chemistry teaching.

探索了一些在化学教学中应用归纳和演绎法来培养创新性思维能力的具体的、可操作的措施。

Methods SABC immunohistochemical method was used to detect the expressions of nm23Hl,VEGF and NF-kBP65 in 82 patients of intraocular and extraocular malignancies.

应用免疫组织化学SABC法检测nm23H1、VEGF和NF-kBP65蛋白在82例眼内外恶性肿瘤中的表达情况。

Chemical synthesis and fermentive method is the major industrial way to produce DHS, and microbial rational modification based on the development of metabolic engineering made the fermentive method more widely used.

工业上一般采用化学合成法和发酵法来生产3-脱氢莽草酸,随着代谢工程的兴起,使得更加理性改造菌株成为可能,这更加促进了发酵法的广泛应用。

MethodsThe tissue microarray and immunohistochemistry SP methods were used to detect the MMP-13 expression in 97 cases of breast cancer and 39 cases of breast fibroadenoma and its correlation with clinicopathologic features was analyzed.

应用组织芯片技术和免疫组织化学SP法检测MMP-13在97例乳腺癌和39例乳腺纤维腺瘤中的表达情况,并分析其与临床病理参数的关系。

It is as soft as nylon and as fluey as acylic.PTT possesses the many advantages of many synthetic fibers and these advantages make its application widly. Currently, the protucts of PTT include carpet, garment, plastic and so on. This paper is about the application of filament CM800 in knitted underwear fabrics. Filament CM800 is bi -component fibers with excellent elastic recovery. According to the performance of knitting fabrics, the relationship of knitting technique and wearablity is researched by orthogonal methods.

与常规化纤相比,PTT纤维具有突出的化学稳定性、回弹性,较好的染色性和色牢度以及良好的抗污性,它综合了锦纶的柔软性、腈纶的蓬松性、将多种纤维的优良性能集于一体,这些优势使它具有广阔的应用空间,目前PTT的产品主要涉及地毯领域、服装领域和工程领域等。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。