应用化学

Methods The study was performed concering the neurons and fibers of SP,CGRP and VIP-immunoreactive substance in three predominant superfical ganglial plexus of the guinea pig heart by immunohistochemical ABC method and observed by light microscope.

应用免疫组织化学ABC法，对豚鼠心脏表面3个主要神经节丛的神经元及神经纤维进行了SP、CGRP及VIP免疫组织化学染色及光镜观察。

The organic/inorganic composites, as new materials that combine the inorganic phase"s rigidity,stability and the organic phase"s toughness and workability, have important theoretical and realisticsignificance in materials science. C_(60) is a brand-new holosymmetry spherical molecule with theunique physical and chemical property, displaying wide implications and applications of physics,chemistry, biology, materials science, medicine, electronics, and so on.

有机／无机复合材料集无机相的刚性、稳定性和有机相的韧性、加工性等诸多特性于一体的一类新型材料，具有重要的理论和现实意义。C_(60)是一个全新的全对称球形分子，具有独特的物理、化学性能，已广泛地影响和应用到物理、化学、生物学、材料学、医学、电子学等许多领域。

Keywords structural chemistry;IChO competition;the senior middle school's;chemistry teaching;application

Article From 辽宁师范大学；课程与教学论硕士论文 2005年度结构化学； IChO竞赛；高中化学教学；应用

Despite evidence implicating prostaglandins as critical factor in male reproductive functions, little is known about expression and function of COX-2 in the male reproductive system. By using immunohistochemistry, RT-PCR and Western-blot, the content of this research included:(1) The expression and localization of COX-2 in mature rat and human testis were examined.(2) We investigated the changes of spermatogenesis and testosterone levels in testis after by using the COX-2 specific inhibitor-rofecoxib at 2, 4 and 8 weeks.(3) To explore the relationship between the intratesticular level of testosterone and expression of COX-2 by completely destroying the Leydig cells of mature male rats with injection of a single I.

本课题应用免疫组织化学、RT-PCR和Western-blot等方法检测以下内容：(1)在mRNA和蛋白水平检测正常成年男性及大鼠睾丸组织中COX-2的表达及定位情况；(2)实验组大鼠持续给予COX-2酶的特异性抑制剂Rofecoxib，分别于用药后2周、4周和8周检测大鼠的精子生成以及睾丸组织内睾酮浓度的变化；(3)另一组实验大鼠给予特异性Leydig细胞灭活剂EDS，在特定时间内抑制睾丸组织内合成的睾酮，于用药后2周和4周，采用免疫组织化学及RT-PCR方法观察大鼠睾丸组织内睾酮浓度变化对COX-2合成的影响。

With the help of the rich and varied supramolecule, organic substances, inorganic substance, polymer and biological substance and so on, it can make various design of modifying layers on the surface of electrode. It includes multivariate, multilayers, polycomponent, micronize, etc. That will make the detection more sensitive and more widely applied in analytic chemistry.

化学修饰电极可利用丰富多彩的超分子、有机物、无机物、聚合物和生物物质等，在电极表面进行各式各样的修饰层设计，包括多元、多层、多组份、微型化等，已期达到高选择和高灵敏的检测，使化学修饰电极在分析化学中得到广泛应用。

Characterize the chemical structure of these compounds using traditional wet chemistry techniques as well as Synchrotron-sourced Fourier transform infrared microspectroscopy

应用常规化学方法和同步红外显微光谱仪鉴定生物活性物的化学构成；杆菌O157：H7 效果和调节瘤胃代谢功能的关系

In summary, a low-cost but high-power microwave chemical reactor with double-magnetron based on noncoherent power synthesis is researched.

以上结果说明基于微波非相干功率合成技术的低成本大功率微波化学反应器的设计方法是合理的，这为低成本的大功率微波化学反应器的设计提供了重要的理论依据，对微波应用在化工领域的推广具有重要的现实意义。

In low permeability boundary oil field, applying this technology can make the field development to be profitable. In Changyuan Oii field, this technology can save a great deal of natural gas, which has significant importance to the extension of field development life and increment of EOR.

在外围低渗透油田中采用常温和低温集油化学剂技术，可使低产区块的开发由经济上不可行变为可行；在长垣油田应用低温和常温集油化学剂技术，可节约大量的天然气，这对延长油田开发周期和提高原油采收率具有十分重要的意义。

Nano titanium dioxide is of favorable biological and chemical inertness, strong oxidizability, low cost and long-term stability against photocorrosion and chemical corrosion. Nano TiO_2 and TiO_2/SiO_2 thin films are used photocatalysis degradation materials and self-cleaing materials, and can kill bacteria and virus.At present it is the hot spot in the research of photocatalysis, and it is of great value in the practical applications.

由于纳米TiO_2具有良好的生物和化学惰性、较强的氧化性能、低成本、以及对光腐蚀和化学腐蚀的稳定性等，故其薄膜不仅能用作光催化降解和自洁净材料，还能杀死细菌及病毒，是目前光催化材料的研究重点，具有广阔的应用前景。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。