序列性

The coding region in the genomic RNA of the Maize Dwarf Mosaic Virus was gained by the way of reverse transcription polymerase chain reaction.

本研究利用RT-PCR对玉米矮花叶病河南标样的基因组RNA进行了全序列测定，并与Potyvirus于中其它病毒进行了同源性分析。

Experimental results show that the proposed method can simulate the infrared sequence images of point targets at anytime, any viewpoint and any Field of View (wavebands range from 4 μm to 26 μm).

该仿真方法可以模拟任意时刻、任意指向和任意光学视场4~26μm的点目标红外图像序列，图像生成实时性好。

CEVd is a non-polyadenylated RNA viroid,but there is a fragment rich in A in CEVd sequence.On the bases of uniplex RT-PCR, a multiplex RT-PCR was developed to detect CTV,CEVd and CTLV simultaneously.

实验过程中，发现不具有ploy_加尾的环状类病毒CEVd，同样可以用oligo反转录的RT-PCR检测出来，通过测序分析，其同源性在96%以上，并发现在CEVd序列中有一个富含A的区段。

Toxocara; Toxascaris; mitochondrial DNA; nad1; PCR; SSCP; sequence analysis

弓首蛔虫；狮弓蛔虫；线粒体DNA；nad1基因；PCR；单链构象多态性分析；序列分析

Torsades de pointes is a special form of polymorphic ventricular tachycardia. It is characterized by continuously changing morphology of the QRS complexes that seem to twist around an imaginary line, rate dependence, underlying QT prolongation and the so called "long-short initiation sequence".

Torsades de pointes是一种特殊形态的多形性心室频脉，其心电图的特徵包括：QRS波的形态会一直改变，彷佛缠绕著一条假想的线；另外它的发作常与心博过缓有关，合并有QT间距延长，及以所谓「长-短」的序列而开始。

Based on different DNA fragments between Z and W chromosome, the developed PCR method has no risk of the mistrial of those sex identification markers depended on W chromosome, and can omit extra internal control or restriction enzyme deigestion. The DNA-based spindlin gene approach can provide a rapid, exact and low cost method for sex identification in poultry.

本研究以PCR检测家禽Z与W性染色体中之纺缍体蛋白基因intron序列，以其长度的差异来鉴定家禽性别，可避免仅检测W染色体性别鉴定标记的误判，且不需再搭配内部对照，更不需结合限制酶切割法即进行家禽性别鉴定。

The pHybLex/Zeo-Idl plasmid and the cDNA library plasmid were sequentially transformed into the yeast swains and screened to obtain Leu2^＋ and Leu2^＋LacZ^＋ clones, with the false positive clones excluded using positive and negative controls, and the plasmid of the true positive clone was sequenced and blasted for homological analysis.

方法PCR方法扩增Id1的编码序列并定向克隆入诱饵质粒pHybLex/Zeo，构建重组诱饵质粒pHybLex/Zeo-Id1，酶切鉴定后转化入酵母株EGY48/pSH18-34，检测重组诱饵质粒有无非特异激活报告基因Leu2、LacZ作用；扩增并提取成人肺组织文库质粒，将文库质粒及重组诱饵质粒转化入酵母细胞，依次筛选Leu2^＋，Leu2^＋LacZ^＋克隆；设置阴性及阳性对照，重复筛选Leu2＋LacZ＋克隆并排除假阳性克隆，获取真阳性文库质粒，进行测序和同源性比对。

The three isolates have four specific replacements in 87 , 88 , 95 , and 119 in S1 hypervariable region, and a unique cleavage site R-X-R-T-G-R in 3' terminal region. These data may be valuable for investigation of molecular basis of the serotyping, tissue tropism and pathogenicity of these virus isolates.

本文的3个分离毒株Q1、J2和T3在S1高变区的87，88，95和119有4个特有的替换，在3'末端有独特的裂解位点R-X5-R-T-G-R，这些结果可能对这些分离毒株的血清型特异序列和组织趋向性和致病性的分子决定区方面的研究有重要的价值。

The sequence analysis result indicated that ompH is of hign homology between different serotypes.

序列分析结果表明，ompH基因在不同血清型之间具有较高的同源性。

Previously, it was found that the hemolytic activity of the extracellular product of V. harveyi was involved in pathogenesis in salmonids, and the most pathogenic isolate, VIB 645, contains two closely related hemolysin genes, i.e. vhhA and vhhB.

已发现哈维氏弧菌对鱼类的致病性与其分泌的胞外产物中的溶血素相关，致病力最强的菌株VIB 645有2个碱基序列非常相似的溶血素基因，vhhA和vhhB。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。