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Suppression subtractive hybridization was used between virulent strain HA9801 and avirulent strain 12. Five fragments of putative virulent genes were found by this method.

并采用抑制性差减杂交的方法对2型毒力菌株所携带的差异基因序列进行了研究,发现了5个新的可能的毒力基因。

The 19 strains of bacilliform bacteria were identified by biochemical tests, part of their 16S ribosomal DNA were amplified, sequenced, and phylogenetically placed.

分离得到得19 株乳杆菌通过菌落、菌株形态观察以及多项生理生化等常规鉴定,并且通过16S rRNA 基因序列测序鉴定,确定这19 株乳杆菌分属于乳杆菌属内专性同型发酵类型中三个不同的种。

The main outputs were validation of the suitability of perennial species for the project area including citrus, pineapple, macadamia, bananas, and palm hearts (Bactris gasipaes Viable cropping systems and sequences were identified.

主要产出的项目区为多年生树种适宜性的验证(包括柑橘,菠萝,坚果,香蕉,棕榈心)可行的种植制度和序列进行了鉴定。

In this paper, first we define a kind of transformation on the sequences of Boolean functions, and then analyze the changes of nonlinearity, algebraic degree and balancedness of Boolean functions under the transformation.

本文首先定义了对布尔函数的序列的一种变换,并分析了进行此种变换后布尔函数的非线性度、代数次数与平衡性的变化情况,最终在此基础上提出一种新的递归构造方法,可以构造出非线性度很高的n元m阶n-m-1次弹性函数。

Mitochondria DNA extracted from bivoltine silkworm pupae was digested by EcoRⅠ into five restriction fragments,of which the 1.9kb fragment was cloned and sequenced.

克隆了二化性家蚕蛹体的线粒体DNA EcoRⅠ酶解1.9kb片段,并进行了序列测定。

The results demonstrated that MC1R gene of bird was relatively conserved. The results of phylogenetic analysis showed that consanguinity relations between mule duck, anas platyrhynchos and branta bernicla, anser caerulescens, anser canagica were close.The status of them were confirmed on zoologyl by means of molecular method.

可见禽类的MC1R 基因编码区序列具有高度的保守性;系统进化分析表明,半番鸭、北京鸭与雪雁、黑雁、皇雁亲缘关系相近,从分子角度验证了它们在动物学分类上的地位。

In addition, homologous alignments and analyses of identical percentage were made between corresponding cDNA sequences to CAL homologs of 8 plants in the same family, Brassicaceae.

另外,对8种十字花科植物CAL同源基因中相应CDNA序列进行了同源性比对和一致性分析,并据此绘制了它们之间的进化树。

The results Showed that twenty isolates have the following biological aspects:① Capability of forming capsule,② Growth at 37℃ within 3days,③ Forming brown colonies on Niger seed agar, CACA agar,④ Urea hydrolysis test positive within 96 hours.⑤ No blue colonies were found on CGB agar.⑥ The genus could be identified by ID32 identification kid, but the species could not.⑦ The sequence of the LSUrDNA D1/D2 region analyzed confirmed to be C. neoformans var neoformans.⑧ Serotype A and AD were found in these isolates, Serotype A was predominant(83.33%),⑨ Some biodiversities of isolates existed.

结果 20株菌皆具有以下特征:①形成英膜,②37℃3天内生长试验皆为阳性,③咖啡酸玉米琼脂和油菊籽培养基皆可以产生褐色菌落,④尿素酶试验96h皆呈阳性,⑤CGB培养基未见蓝色菌落生长,⑥ID32鉴定板可以鉴定到属,种的鉴定结果各不相同,⑦大亚基核糖体DNA的D1/D2区域序列分析证实皆为新生隐球菌的新生变种,⑧血清型仅见A和AD二型,以A型为多数(83.33%),⑨20株临床分离株随机扩增DNA多态性分析有一定的生态多样性。

Avian influenza virus was isolated from the caecal tonsil, faeces, and kidney of the domestic ducks. The isolated virus was identified as a highly pathogenic H5N1, with a haemagglutinin proteolytic cleavage site deduced amino acid sequence of ... QREKRKKR/GLFGAIAG ... In order to determine the pathogenicity of the isolate, eight 6-week-old specific pathogen free chickens were inoculated intravenously with the virus, and all birds died within 24 h after inoculation.

病毒抗原可以从鸭的盲肠,扁桃体,肾脏及粪便分离出来,并且确定这种病毒是高致病性的H5N1,还从血凝素蛋白分解位点推断出病毒的氨基酸序列,为了了解独立群的发病机理,对8只6周龄鸡静脉注射这种病毒,结果所有的均在注射后24小时死亡。

Aian influenza irus was isolated from the caecal tonsil, faeces, and kidney of the domestic ducks. The isolated irus was identified as a highly pathogenic H5N1, with a haemagglutinin proteolytic cleaage site deduced amino acid sequence of ... QREKRKKR/GLFGAIAG ... In order to determine the pathogenicity of the isolate, eight 6-week-old specific pathogen free chickens were inoculated intraenously with the irus, and all birds died within 24 h after inoculation.

病毒抗原可以从鸭的盲肠,扁桃体,肾脏及粪便分离出来,并且确定这种病毒是高致病性的H5N1,还从血凝素蛋白分解位点推断出病毒的氨基酸序列,为了了解独立群的发病机理,对8只6周龄鸡静脉注射这种病毒,结果所有的均在注射后24小时死亡。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

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这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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