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The result of uroscopy of the proband was strong positive. There was a novel deletion mutation of c.876-877 del TC in the coding region of exon 6 of IDS gene, which was a hemizygous mutation. However, the mutation of his mother and sister was a heterozygous mutation. Detection of the exon 6 of IDS gene showed that the mutation was not found among normal controls and other patients with MPS I, IV, and VI other than MPS II. Homology comparison of amino acid sequences from different species showed that the phenylalanine glutamine of the mutation site of c.876-877 del TC located in p.292-293 was highly conserved. The activity of IDS enzyme of the proband was only 2.3 nmol/4 h/mL, which was much lower than normal; but the activity of IDS enzyme of his father, mother and sister was 641.9 nmol/4 h/mL, 95.8 nmol/4h/mL and 103.2 nmol/4h/mL, respectively.

结果显示:先证者尿检呈强阳性;其IDS基因exon 6编码区内存在c.876-877 del TC新缺失突变,为半合子突变,而其母、其姐为杂合突变;正常对照和其他非II型MPS患者的IDS基因exon 6的检测结果均未发现该突变;不同物种氨基酸序列的同源性比对显示: c.876-877 del TC突变所在的位置即p.292-293的苯丙氨酸谷氨酰胺高度保守;酶活性测定的结果显示:先证者的IDS酶活性仅为2.3 nmol/4 h/mL,大大低于正常值,而其父的为641.9 nmol/4 h/mL,其母的血浆酶活性为95.8 nmol/ 4h/mL,其姐的为103.2 nmol/4 h/mL。

The deduced amino acid sequence of LAR from tea plant showed high identity with that of other plants, for instance 71%, 70% and 68% with Vitis vinifera, Gossypium arboretum and Fragaria ananassa, respectively.

同源性分析表明茶树无色花色素还原酶基因编码的氨基酸序列与其他植物具有较高的相似性,例如与亚洲棉、草莓和葡萄的相似性分别为70%、68%、71%。

Compared with 11 other isolates reported in GenBank, there was slightly variation in the nucleotide sequence of Omp25 gene of wapiti's isolate. The identities of nucleotide sequence and deduced amino acid sequences among 11 strains were 99.1%-99.4% could 99.2%-99.5% respectively.

与GenBank中已经登录的布氏杆菌其他11个分离株相比,马鹿布氏杆菌分离株Omp25基因变异较小,以散在的点突变为主,Omp25基因核苷酸和推导氨基酸序列的同源性分别为99.1%-99.4%和99.2%-99.5%之间。

In this paper, we have estabished the closed convexities and connectedness of the cone weak subdiffrerential of limit mapping of set-valued mapping sequence.

在文[,4]基础上,给出了集值映射序列的极限映射的锥弱次微分的闭凸性和连通性。

The thought on weighted sampling about pre-frame not only can be applied in object tracking based sequential images, but also can be extended to other fields. The method is universal and practical.

该算法提出的引入前帧加权采样思想不仅仅局限于基于序列图像的跟踪,而且可以推广到其它相关的领域,具有普适性和实用性。

Phylogenetic analysis showed that sugarcane was firstly clustered with maize, then with sorghum and Zoysia japonica, and then with rice.

该基因片段具有其它植物GA20氧化酶基因中存在的保守区;同时,与多种单子叶植物的GA20氧化酶基因的核苷酸和氨基酸序列的同源性在80%和75%以上。

It can adaptively set the thresholds of motion selection according to the characteristics of the unplanned motion sequence.

针对在线手眼标定算法的鲁棒性,提出了一种根据运动序列的特点自动设定运动选择阈值的在线手眼标定算法。

However, this algicidal substance was unstable at different acid and alkali concentration, it could improve algicidal activity at neutral, alkaline, but reduce algicidal activity at high acid concentration;(4) according to the studies on the mophorlogical characters, the physilogical, biochemical characters and the sequence analysis of 16S rDNA of strain W5, it was identified as a Enterobacter aerogenes.

但酸碱稳定性不如热稳定性强,在强酸条件下,溶藻活性有所降低,中碱性条件下可以增强其溶藻活性;此溶藻物质活性成分是水溶性的,不溶于常见的有机溶剂(4)通过形态学特征、生理生化试验以及16S rDNA序列分析,W5菌鉴定为产气肠杆菌。

Jinan177 (JN177) and Agropyron elongatum using genomic PCR method. All six sequences have high similar to those of wheat and related grasses reported before.

对这些基因序列进行分析,发现与以往克隆的LMW-GS基因具有高度的同源性。

Another Amorpha fruticosa cDNA clone encoding 4-coumarate:CoA ligase (4CL), a key enzyme of phenylpropanoid metabolism related to lignin forming, was cloned ,too.A full-length UGPase cDNA was obtained.

所获得的紫穗槐尿苷二磷酸葡萄糖焦磷酸化酶基因UGPA长1832bp,其中开放阅读框1416bp,编码471个氨基酸,与其它植物的UGPase基因的序列相比有较高的同源性。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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