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The deduced amino acid sequences of HbHEX1 showed high identities of 92%,77% and 72 % to those of the homeobox protein from Ricinus communis,Solanum chacoense and Daucus carota.

该氨基酸序列与蓖麻、马铃薯、胡萝卜的同源异型盒蛋白的同源性分别为92%、77%和72%。

The deduced amino acid sequences of HbHDT1 showed high identities (65%, 59%, 50%, 45%, 43% and 40%, respectively)to those of the histone deacetylase from Ricinus communis, Populus trichocarpa, Solanum chacoense, Trifolium pretense, Glycine max and Triticum aestivum.

该氨基酸序列与蓖麻、毛果杨、马铃薯、红花苜蓿、大豆、小麦的HDACs家族成员的同源性分别为65%、59%、50%、45%、43%和40%。半定量RT-PCR分析结果表明HbHDT1在巴西橡胶树的花、愈伤组织、胚、叶、芽、胶乳中均有表达。

This gene contained 1 530 bp and encoded 510 amino acids, named IbMIPS-1. Sequence analysis indicated that IbMIPS-1 had high homology with Ricinus communis MIPS gene, Nicotiana tabacum MIPS gene, Sesamum indicum MIPS gene and Glycine max MIPS gene.

该基因由1530个碱基组成,编码510个氨基酸,与蓖麻、烟草、芝麻、大豆肌醇-1-磷酸合酶的氨基酸序列比对表明,其同源性很高。

it is a challenging task to track the object effectively and robustly in sequence image.

序列图像中运动目标跟踪的有效性和鲁棒性是一个非常富有挑战性的课题。

The 5'coding region of its eDNA was optimized according to the codon bias of Pichia pastoris to make it secretively express in Pichia pastoris with higher performance. Two-step DNA synthsis was used to synthesize the eDNA sequence being optimized of ADTZ. OPT-ADTZ was connected with constitutive plasmid pGAPZaA to construct the reecombinant plasmid pNOA. pNOA was linearized and then transformed into Pichia pastoris GS115. Then codonoptimized ADTZ was constitutively and secretively expressed in Pichia pastoris.

为了使重组蛋白rADTZ能在毕氏酵母中高效分泌表达,根据毕氏酵母密码子的偏好性对rADTZ的5'末端的编码区域进行了优化,利用two-stepDNAsynthsis技术合成出ADTZ优化的基因序列OPT-ADTZ,并与组成型表达载体pGAPZaA连接,构建重组质粒pNOA,线性化pNOA后转化至毕氏酵母GS115中,实现了密码子优化的rADTZ组成型分泌表达。

Aim: To set up the methods of polymerase chain reaction-sequence specific primers for human ABO blood group, HAB secretor status and MN blood group genotyping and study the polymorphism of ABO blood group genes, HAB secretor status genes in part of Han population in Chinese and MN blood group genes in random blood donors and study the application of ABO genotyping in clinical transfusion and genetics.

目的:采用聚合酶链反应-序列特异性引物扩增基因定型方法,对ABO血型、HAB分泌型和MN血型系统基因定型,研究ABO血型、HAB分泌型和MN血型基因多态性分布特征及ABO血型基因定型在临床输血、遗传分析中的应用。

Eleven differentially exp ressed fragmentswere confirmed by bulked segregant analysis and reverse Northern blotting, further cloned and sequenced.

经过BSA 分析和反向Northern杂交鉴定,获得了11个差异表达的cDNA片段,并进行了克隆、测序和序列同源性检索分析。

The methods of bulk segregant analysis , randomly amplified polymorphic DNA and sequence characterized amplified regions were used to analyze F〓 individuals of the hybrid IR24×78-15 and molecular genetic markers linked to Xa-min gene were identified.

同时,以IR24和78-15为亲本进行杂交(IR24×78-15),并采用分离群体分组分析法、随机引物扩增多态性DNA和序列特异性扩增区筛选与Xa-min连锁的分子标记。

Results: Typical DNT was located in cortical or subcortical white matter, with cystic or semiliquid structures. On MR images appeared as lesions with inhomogeneous signal intensities, consistent MR feature in all cases were the presence of one or multiple areas of moderately or markedly reduced T1-signal and markedly or moderately increased T2-signal, with moderate enhancement.

结果:典型的DNT位于皮层或主要位于皮层区,肿瘤以囊性成分为主、病灶在T1WI为低信号,T1WI为高信号,其ADC值增高,FLAIR序列上病灶边缘和分隔为高信号改变,增强扫描病灶实质结节或分隔可见轻度强化。

Under the strict complementaritycondition at the accumalation points of the sequence of iterations, the global convergenceof the algorithm is proved.

由此建立了一种基于SQP方法的新算法,且在迭代序列的聚点满足非退化假设条件下,获得了算法的全局收敛性。

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