序列性

The sequence analysis indicated that the deduced amino acid sequences of the cDNA sequences shared high identity with OR83b orthologue chemoreceptor sequences from other previously reported moths.

通过在GenBank中进行序列的同源性比较，发现这2个鳞翅目化感蛋白新序列与已知蛾类昆虫的OR83b类化感蛋白氨基酸序列具较高的同源性。

Sequence analysis showed that the coding region of BDNF gene from malayan bear was highly homologous with those of human and giant panda counterpart genes (94% and 99% homology, respectively).

对该基因所作的序列分析表明，马来熊和人BDNF基因编码区的核苷酸序列同源性为94%，而与大熊猫BDNF基因的核苷酸序列同源性高达99%。

Segments of Adh gene in azuki bean (variety KS 5), covering an interval between partial intron 3 and intron 9, were amplified and sequenced with two pairs of primers. The result has proven the viability of using conserved sequences in a completely sequenced gene of one species to clone same gene in another species. In the comparison between Adh genes of azuki bean and pea, high sequence similarity (about 80%) in exon and long length difference (up to 116 bp) in intron were found. Besides, the edge regions of Adh introns with GT/AG counterparts and more conserved sequences than central regions was observed in both species.

利用两组引子对完成红豆(高雄5号)Adh基因之部分解序，其范围包括部分intron 3至intron 9之间的区域，也印证利用基因之已知保守序列可选殖另一物种之相同基因，红豆与豌豆之Adh基因序列比对结果显示两者之exon均较intron有较高之GC百分比(分别为41.0 ~ 48.7%及23.3 ~ 35.2%)，且两者在exon区域之DNA序列相似程度约80 %，而在intron区域中则有明显的序列差异存在(长度差异最高可达116 bp)，但是intron外侧序列均具有GT/AG组成且相对较中央序列具有保守性。

It was known that ORF of SeMNPV egt was 1572bp long and encodes 523 amino acids through DNAstar software analysis. TATA boxes lies at 48~53 and 68~72bp of the 5'-nonencoding region upstream of a translational start site. Comparing with seven kinds of nuclear polyhedrosis virus and one kind of granulosis virus, it indicated that the SeMNPV egt gene is the highest match the Agrotis setegum nuclear polyhedrosis virus.It has 75% nucleotides and 79% amino acid identity.

采用DNAstar软件分析，与7种核多角体病毒及1种颗粒体病毒egt基因的同源性比较显示，SeMNPV egt基因与核多角体病毒egt序列同源性较高，其中与黄地老虎核型多角体病毒同源性最高，核苷酸和氨基酸序列同源性分别为75%和79%。

Sequences of modified genes GOX and CP4-EPSPS in GM canola and that of modified gene Cryla in GM cotton were decoded and the conservative sequences of exogenous resistant genes: PLRVrep, PVYcp and CryⅢA in GM potato: New leaf〓 PLUS and New leaf〓 Y were confirmed using the modified exogenous gene decoding technique and isogenous sequence similarity BLAST analysis. Bases on DNA sequences studied above, conventional PCR primers were designed and selected in an optimized way and the conventional PCR detection protocol for exogenous resistant genes in 19 GM crops was established.

本研究采用反向PCR克隆—测序测定未知序列技术，针对转基因玉米（MON810、BT11、BT176、GA21、T25、CBH-351）六个品系、转基因大豆GTS 40-3-2品系、转基因油菜RT73和MS8两个品系、转基因棉花MON531和MON1445两个品系测定出品系鉴定的边界序列；采用外源修饰基因序列的破译方法和BLAST同源性序列分析技术，针对转基因作物中修饰的外源抗性基因进行破译研究，破译出转基因油菜中修饰的GOX基因、修饰的CP4-EPSPS基因和转基因棉花中修饰的Cry1A基因的序列，并确定了转基因马铃署New leaf〓PLUS和New leaf〓Y两个品系中PLRVrep、PVYcp和CryⅢA外源抗性基因保守序列。

The results showed that the gene and amino acid sequence homology between seven species are over 44.3% and 47.90/o, respectively. In addition, conservation of three sequences: EQCGSQAGGALCP, CCSQFOWCGST and CQSQC in the 40 amino acids sequence at the beginning of the N-terminal chitinase mature protein is very high, The conservation of eight Cys at the sites of 3,12,17,18,24,31,36,40 is 100% Finally, the chitinase signal peptide gene from Phaseolus vu?

结果表明七种基因之间有44.3％以上的同源性，蛋白之间有47.9％以上的同源性，而且在成熟蛋白N端前40个氨基酸序列中分别位于1～13、17～27和36～40位的EQCGSQAGGALCP、CCSQFGWCGST和CQSQC序列区保守性很高，尤其是位于第3、12、17、18、24、31、36、40位的八个半胱氨酸的保守性达到100％。

Meanwhile, comparison of sequence between AlMV-Ch与AlMV425 show that the homology of the nucleotide and deduced amino acid sequences of the replicase are 97.8% and 97.6% respectively, but the homology of 3'-end un-coding reagin is 98.2%.

AlMV-Ch与AlMV425进行了同源性比较，结果表明复制酶基因编码区核苷酸序列同源性为97.8%，而推测的氨基酸序列同源性为97.6%，3′非编码区的同源性为98.2%。

The sequences were compared with the known sequences in Genbank with BLAST software. P-ACC/M-GGA200 showed 95% homology to a part of AFLP sequence which linked to fusarium head blight resistance gene fragment (AF389881). The P-AGG/M-GGT_(251) has an ORF, and it had homology to a hypothetical protein of Oryza sativa.

根据BLASTn同源性比较，P-ACC/M-GGA_(200)与GenBank中与抗小麦赤霉病连锁的一段AFLP序列(AF389881)同源性达95%；P-AGG/M-GGT_(251)序列中含有一个开放阅读框，根据BLASTp同源性比较，与GenBank中日本栽培稻一未知蛋白(XP 483138)有同源性。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对，结果显示，PPV-SC1 NS1与其他的PPV NS1的同源性较高，仅存在个别的差异，分别是第39位A→G，第153位T→C，第175位A→G，第1117位A→C，第1535位A→C；同源搜索比较表明，PPV-SC1与PPV NS1同源性可达98％、99％，与其他的细小病毒NS1基因也存在很大的保守性；密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性；PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段，用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区，返回的结果并没有得到有显著意义的跨膜区的存在；根据基于motif数据库的结构域预测，PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP／GTP结合位点，该蛋白还存在16个蛋白激酶C磷酸化位点，21个酪蛋白激酶2磷酸化位点，3个cAMP-／cGMP依赖蛋白激酶磷酸化位点，PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域，推测NS1可能与POX_D5有类似的功能。

In addition, basing on the tight-binding model of the single electron and transfer matrix method, we study the charge transfer properties of Fibonacci sequence and discuss specially the dependent of electronic transmission on energy and the length of the sequence. We find some resonant peaks can survive in relatively longer Fibonacci sequences than random sequences, which also implies there are long-range correlations in Fibonacci sequences.

从Anderson紧束缚模型出发，采用传输矩阵方法研究了Fibonacci序列的电子输运特性，讨论了输运系数对能量及其序列长度的依赖关系，发现随着序列的长度增加，具有好的透射性的电子态数量有所减少，但相对于随机序列而言，共振能态可以在更长的序列中存在，进一步证明了Fibonacci序列中存在有较强的长程关联行为。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。