序列性

The cytoderm of the thalli contained glycerol diether derivatives with halophilous archaea characters. The shape of the thalli was spherical and the colony was orange red, which was identified as halophilous archaea, Halorubrum genus based on the physiological and biochemical identification and l6S rDNA gene sequence homologous analysis.

结果］SYM菌株能在饱和NaCl浓度下生长，菌体细胞壁含有嗜盐古细菌特征性的甘油二醚衍生物，菌体形态为球状，菌落显橙红，经生理生化和16 SrD-NA基因序列同源性分析鉴定为嗜盐古细菌嗜盐深红菌属。

The inter-simple sequence repeat approach was employed to detect the genetic diversity of 12 Eichhornia crassipes collected from different water area in Fujian Province.

在建立内部简单重复序列分析方法的基础上，以采自福建省主要河流和福州内河的12份水葫芦样本为材料，筛选出20个可扩增出清晰且具多态性片段的ISSR随机引物，通过PCR扩增，探讨其遗传多态性和亲缘关系。

Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具，分别以人丁酰胆碱酯酶(1p0i：A)和果蝇乙酰胆碱酯酶(1d×4：A)的蛋白晶体结构为模板，对嗜虫书虱2个AChE的三维结构进行同源建模，并在三维结构中发现了AChE的酶解活性位点，证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少，在GenBank中没有可用作内参基因的序列，因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号：FJ041117)，该片段长度为822 bp，编码273个氨基酸残基，同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

The amino acid sequence of the N-terminal portion of the StdA, the major fimbrial subunit of Std fimbriae, reveals no homology to that of the non-Salmonella strains and is conserved within Salmonella strains.

Std线毛的主要线毛次单位为StdA，而其N端的胺基酸序列仅与沙门氏菌属有同源性，在其他非沙门氏菌属中则无同源性。

Because more than 75. 4% regions were occupied by heterochromatin on the sex chromosomes of spiny eel, its X library should be a good tool to isolate the heterochromatic highly repetitive sequences DNA and study its origin and evolution.

在刺鳅的性染色体上，75.4％以上的长度都是异染色质区域，因此，刺鳅X染色体文库也为分离获得鱼类性染色体上异染色质重复序列，探讨其起源和进化规律提供了良好的实验材料。

A chimeric primer was designated in which segment A near 3' end is complementary to HB plus strand just before the DR2 region gap, and segment B near 5' end is consensus to part of the human immunodefficient irus genomic sequence, without homogenetic relationship to HB genome.

设计一嵌合引物，使之3'端的A片断与HB正链互补，并恰好位于DR2缺口之前；5'端的B片断与人类免疫缺陷病毒的基因序列具有同源性，与乙型肝炎病毒的基因组无同源性。

Restrictive changes of TCR Vp repertoire and clonal expanded T cells could be found in peripheral blood T cells from patients with T-ALL. However, the clonal expanded T cells' property (clonal expansion leukemic cells or leukemia antigen-specific expansion T cells) should be further characterized. It may play a certain role on detection of minimal residual disease and design of anti-leukemia idiotypic vaccine. The dominant utilization of TCR VjJ repertoire could be found in peripheral blood T cells from patients with B-ALL, meanwhile clonal expansive T cells were existed. It may be a feature of the host immune response for leukemia-associated antigen. The clonal expansive tendency of T cells in V021 and VP23 subfamilies was rather obvious, which may be correlated with certain malignant B-cell clone. The CDR3 sequences of monoclonal expansive T cell in T cell strains were different, which was the base of developing DNA vaccine.

T-ALL患者外周血T细胞的TCR Vβ谱系出现限制性改变，均可检测到克隆性增殖T细胞，尚需进一步鉴定其性质(肿瘤性或抗原特异性增殖)，对于研究微小残留病变检测和设计抗白血病独特型疫苗均有一定的意义。B-ALL病人外周血T细胞的TCR Vβ谱系呈现优势利用的特点，并存在克隆性增殖的T细胞，这可能是机体对白血病相关抗原产生的特异性免疫反应；Vβ21和Vβ23亚家族T细胞发生克隆增殖改变的趋向性比较明显，可能与B-ALL相关抗原刺激有关。T细胞株中检测到的肿瘤克隆其CDR3序列具有高度的特异性，是构建DNA疫苗的基础。

Focusing on the PGE enrichment mechanism in the basic-ultrabasic intrusions in the Panxi rift, firstly, through the interpretation of TM photograph, data processing of regional geochemical exploration and geophysical prospecting results of gravity, magnetic force and seism, we analyzed the controlling effect of structure and magmatic movement on the PGE mineralization; Secondly, through the study of volcanic apparatus, petrography, measurement of comprehensive geochemical section, isotope tracing and dating of typical PGE-rich melanocratic rock series, we discussed the sources of the PGE-bearing mafic-ultramafic intrusions and basalts, their timing, evolvement sequences, the geochemical processes of magma crystallization and fractionation, and the relationship between magma evolution and structural development; Finally, through the analysis of PGE and chalcophile elements concentrations in the melanocratic rock series and the contrastive study with the Noril''sk magmatic Ni-Cu-PGE sulfide deposit, its related continental flood basalts and Siberia Traps, we discussed PGE mineralization characteristics and its enrichment mechanisms in the melanocratic rock series in the Panxi rift.

本项目首先围绕川西地区主要的PGE矿床及伴生矿的地质环境和有关的地质构造背景，通过TM卫片解译、区域化探和重、磁及地震物探资料分析，总体把握构造及岩浆活动对PGE成矿的控制作用；然后通过对典型富PGE暗色岩套的火山机构地质构造、岩石岩相学、地球化学、同位素定年和示踪等方面的工作，分析本区基性－超基性含矿岩体与喷出岩之间的成岩物质来源、侵入时代及演化序列，研究该区同一岩浆房分异结晶的地球化学过程及其随构造发展的耦合关系；最后通过对暗色岩套PGE地球化学剖面分析以及与俄罗斯诺里尔斯克陆内裂谷溢流型玄武岩中PGE矿化的岩石－构造环境对比研究，探讨该区暗色岩套中的矿化特点和富集部位及其PGE富集机制。

Mellifera ,with 91% amino acid identity.

同时根据氨基酸序列用GENETYX程序绘制了 7种Hya的分子系统树，并根据同源性比较分析了 7种Hya的保守性、结构与功能的关系

Objective To explore the relationship between 3'-variable numble of tandem repeats (3'-VNTR) of apolipoproteins B and cerebral hemorrhage with family history in Changsha Han Chinese.

目的 探讨载脂蛋白B(apolipoproteins B， apoB)基因3'端可变数目串联重复序列(3'-variable numble of tandem repeats， 3'-VNTR)多态性与长沙地区汉族人群家族聚集性脑出血的关系。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。