序列性

The nearly full-length CP sequence of 308 aa of SCMV-NCH was compared with the counterpart of SCMV, Sorghum mosaic virus, Maize dwarf mosaic virus; Johnsongrass mosaic virus, Zea mosaic virus and Pennisetum mosaic virus, the six virus species in SCMV subgroup in Potyvirus, respectively. A high level of sequence homology (86.2%) was observed between SCMV-NCH and SCMV-A, while only 56.4% to 72.2% sequence homology was found between SCMV-NCH and the other five virus species.

与SCMV亚组SCMV、高粱花叶病毒、玉米矮花叶病毒、约翰逊草花叶病毒、玉米花叶病毒、白草花叶病毒等6种病毒代表性株系外壳蛋白氨基酸序列同源性比较结果显示，SCMV-NCH与SCMV的同源性最高(86.2%)，与其他5种病毒同源性相对较低(56.4%~72.2%)。

Total RNA was isolated from phloem of young shoots and retro-transcripted to cDNA by RT-PCR with specific primers, and the amplified cDNA fragments were sequenced for identification of subtypes. An effective RT-PCR technique was established for detecting citrus viroid types, and three types were recognized, i.e.

以田间柑橘枝条韧皮部为试材，提取总RNA，反转录成cDNA，再用不同类型类病毒的特异引物进行PCR扩增，鉴定出各个样品所携带的柑橘类病毒类型；通过测序分析各类型的序列，并与国内外报道的序列进行同源性分析，确定所测样品的亚型。

The three isolates have four specific replacements in 87, 88, 95, and 119 in SI hypervariable region, and a unique cleavage site R-X-R-T-G-R in 3 terminal region. These data may be valuable for investigation of molecular basis of the serotyping, tissue tropism and pathogenicity of these virus isolates.

由于SI蛋白序列99－12川立氨基酸之间的序列可能对病毒的致病性起重要作用，SI蛋白5，高变区（56－69；117－131）与中和表位和血清型密切相关，这些区域在IBV血清型和基因型间起联系作用。

The deduced maize amino acids share high sequence homology with cyMDH from other species, such as O. sative, M. erystallinum, and G.

序列联配与树状分析结果表明，该玉米cyMDH序列与多个物种的cyMDH基因具有高度的同源性。

Region of the homeodomain shows homology with the helix-turn-helix motif of prokaryotic repressors.

图29.10比较了三个螺旋区的序列，序列保守性最多的部分在第三个螺旋。

Researches on proteid reveal that VSV N gene encoding nuclear protein is a polypeptide which is made up of 422 amino acid and has a strong hydrophility. Comparing its homogeneity with that of VSV wild strains, it shows that the nuclear protein is highly conservative due to the high homogeneity of sequences which is above 98. 8%. It provides a basis for establishing clinical VSV diagnostic method.

蛋白质分析发现，VSV N基因编码的核蛋白是由422个氨基酸组成的多肽，具有较强的亲水性，同VSV野生型病毒株的核蛋白同源性比较，其氨基酸序列的同源性高达98.8%以上，证明VSV核蛋白具有很好的保守性，为临床上建立VSV诊断方法奠定了基础。

New laccase gene and its full-length cDNA were clone from genomic library and equalized cDNA library of Ganoderma lucidum. Similarity between the sequence and other laccase gene were analyszed with NCBI blast, the results show that the new gene belong to multicopper oxidases gene family and have 60-70% Similarity with other laccase gene.

从灵芝的基因组文库和cDNA均一化文库中克隆了灵芝的漆酶基因和全长cDNA，并通过NCBI Conserved Domain Search氨基酸序列的相似性分析及与其它真菌漆酶之间的相似性分析，证明得到的蛋白是一种新的multicopper oxidases，其蛋白质序列与其它已克隆漆酶基因的同源性在6 0～7 0％之间，并对论文克隆的漆酶基因的启动子和终止子进行了分析。

Using method of variable difference, random error was parted from general error.

因此，提出了从非平稳性序列转变成平稳序列的方法。

After pathogenic fungi identified,we found 26 of them can cause cotton leaf spot. The 26 pathogenetic strains were carried out morphological and molecular identification of the firm. The initial identification of the pathogens is through the morphological observation such as the determination of growth, colony morphology, conidia and conidial stalk.

通过对生长量的测定，菌落形态，分生孢子和分生孢子梗等形态学观察进行形态学鉴定；通过PCR扩增真菌核糖体基因转录间隔区序列并测序，并将序列在GenBank上进行同源性比对和构建系统系统发育树等方法进行分子鉴定，鉴定出这26株真菌分属链格孢属、镰孢属、黑孢属、刺盘孢属、匍柄霉属等5个属。

The generating algorithm is derived.It is verified that the GMW PC sequences have balance,optimal auto/cross correlation functions,and very large linear spans and can be used in the CDMA spread spectrum communication and cryptography.

给出了GMW相控序列的生成算法，证明了GMW相控序列均满足平衡性，具有优良的相关特性和极大的线性复杂度，可适用于CDMA扩频通信和保密通信系统中。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。