序列性

An identical nucleotide sequence in various passages was obtained by analysis using DNASTAR software, to which the homologies of nucleotides of E2 gene of passages 14, 16, 17, 18 and 23 were 100%, 100%, 99.8%, 99.8%and 99.6%, and those of amino acids were 100%, 100%, 100%, 99.3%and 99.2%, respectively.

结果 Matsuba株E2基因全长846bp，传至23代时仍有很高的同源性，第14、16、17、18和23代与一致序列的核苷酸同源性分别为100%、100%、99.8%、99.8%和99.6%，氨基酸同源性分别为100%、100%、100%、99.3%和99.2%。

One of these clones,M79 was selected for further study.M79encodes a typical MADS box protein,and it shares high homology with many ofthe MADS box genes in plants.

对其中一个克隆M79的DNA序列和蛋白质序列分析表明，M79基因编码多肽含有一个典型的MADS box和K-box，与植物中的许多MADS box基因产物有很高的同源性。

Sequence analysis and homology searches of public data bases, however, revealed no published sequences significantly similar to the sequence of the fragment, precluding a predicition of the potential function of the sequence.

但DNA序列分析和数据库检索表明，该片段与迄今发表的核酸序列均不存在显著的同源性，因此尚无法预测其潜在的功能性。

As a result,the OmpK genes not only were distributed widely in all tested Vibrios,but also shared quite high similarity in nucleotide sequences and deduced amino acid sequences.

序列分析还表明，每一种弧菌OmpK基因都有一段特异性序列，可用于设计核酸探针或特异性引物来诊断、检测哈维氏弧菌等海水鱼致病性弧菌。

Homologous analysis of the deduced amino acid sequences were performed by BLAST and subsequently compared with GenBank data.

根据衣藻、矮牵牛花、Pisumsativum等真核生物hsp70a氨基酸高度保守序列设计两对简并引物，以热休克盐藻cDNA为模板进行巢式PCR扩增，产物经T-A克隆转化至JM109大肠杆菌中，经筛选后测序，将测序结果推导成氨基酸序列进行同源性分析，获得了盐藻热休克蛋白70a cDNA片段。

According to the conserved regions of other known insects, a pair of specific primers were designed and the RT-PCR method was used to amplify the partial sequences of HSP70 from cotton aphid, then analyzed the nucleotide and deduced amino acid sequence.

根据已知昆虫HSP70基因的保守性区域，设计特异性引物，采用RT-PCR方法扩增棉蚜HSP70基因的部分序列，测序后进行核苷酸和推导的氨基酸序列分析。

Connective reaction: 6.5μl PCR productions, pMD18-T vector 0.5μl, Ligation SolutionⅠ5μl, T4 DNA Ligase 0.5μl, 10×T4 DNA Ligase 1.5μl were connected overnight at 16℃. 5、transformation of vector: DH5α was prepared by CaCl and mixed with connecters, cultured on Amp+LB overnight at 37℃.

双酶切及PCR鉴定。1%琼脂糖凝胶电泳。7、序列测定：测序结果与Gene Bank中报道的序列同源性为99.1%，说明变链ldh基因及同源区基因已克隆成功；命名该重组质粒为pMD18-T-ldh。

Excess kurtosis and fat tail characteristics in time series of interest rates indicates that the series is a nonlinear stochastic process which can be caused by conditional heteroscedastic or caused by long memory.

金融时间序列呈现出的尖峰肥尾的特征既可以由条件异方差性引起，也可以由长记忆过程引起。对于我国的市场利率时间序列而言，尖峰肥尾的特征同样普遍存在，但目前主要是用ARCH类模型来拟合。

Methods:The peptide sequences of human CYP1A2 were analyzed according to its bioinformatics, and synthesized on the basis of hydrophilicity, antigenicity, accessibility and flexsibility. The synthesized peptides were crosslinked with keyhole limpet hemocyanin, which was used to immunize rabbits for production of Ab.

利用生物信息学方法分析CYP1A2蛋白的序列，根据亲水性、抗原性、柔韧性及表面性等指标选择多肽序列，合成CYP1A2多肽，与载体蛋白钥孔戚血蓝素（Keyhole limpet hemocyanin， KLH）偶联，免疫日本大耳白兔制备抗CYP1A2抗体。

A 1.8 kb fragment of 3' terminus of the viral RNA was amplified by RT PCR, cloned and its sequence was determined. Sequence comparisons showed that it shared 71.5%～99.1% homology with isolates of sugarcane mosaic virus, 67.8%～68.5% with sorghum mosaic virus and 38.4%～48.4% with maize dwarf mosaic virus, indicating that the pathogen of this disease on maize in Hangzhou was sugarcane mosaic virus.

病毒RNA13'端序列(1.8kb)与甘蔗花叶病毒同源性最高，达 71.5 %～ 99.1%，与高梁花叶病毒同源性次之，为 6 7.8%～ 6 8.5 %，与玉米矮花叶病毒同源性最低，仅为 38.4%～ 48.4%，从而初步认为此病害由SCMV引起。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。