序列性

Sequence analysis revealed the presence of a 735 bp open reading frame encoding 244 amino acids protein, which shares 32.9 %~49.2% amino acids sequence similarity to PRAI proteins from other species of Saccharomycetales.

序列分析显示，该基因编码区全长735 bp，编码的磷酸核糖氨基苯甲酸同分异构酶氨基酸序列与其他酵母来源的PRAI蛋白同源性在32.9%~49.2%之间。

All of the 58 restriction endonucleases were used to investigate the mtDNA diversity. Four restriction endonucleases, DraⅠ、SnaBⅠ、SspⅠ and Vsp Ⅰ, were polymorphic. Based on presence or absence of the the 215〓 SspⅠ site, all of the 13 mtDNA sequences were divided into two groups.

利用DNAClub软件对AT富集区和tRNA〓基因的DNA序列进行了模拟酶切，所有的58种内切酶中有10种内切酶在13个材料中有酶切位点，仅4种酶DraⅠ、SnaBⅠ、SspⅠ和VspⅠ表现出多态性，且主要位点差异在于SspⅠ酶切位点的有无（序列的第215位的C/T）。12个品种中有4个品种（豫早1号、豫早2号、鲁红、33）没有此酶切位点，另外8个品种有此酶切位点，野柞蚕也没有此酶切位点。

The StLTPal gene is well conserved in the coding region with 60-92% identity at the amino acid level, and 75-85% identity at the nucleotide sequence level compared with other Solanaceae nsLTPs.

基于氨基酸序列构建的系统发生树揭示，该基因与其他茄科nsLTP具有高度保守的序列相似性，核酸和氨基酸水平分别具75%~85%和60%~92%的同源性。

A pair of primers were designed and synthesized based on the nucleotide sequence of coat protein gene of TAV from England.

将其克隆到 pGEM Teasy中，经酶切和序列分析表明所克隆的是TAVCP基因，与已知的 6个株系相应序列同源性均在 96 。4%以上

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

To investigate the mechanism of wood formation and lignin biosynthesis, it is important to understand the function and characteristic of OMT. In the present study, a set of primer was designed based on the conserved region of OMT genes from several woody and non-woody plants, to clone the counter part gene by PCR and RACE from Chamaecvparis formosensis Matsum., Chamaeeyparis obtusa var. Jormosana, Abies kawakarnii, Tsuga ehinensis var. Jormosana and Taiwania eryptonierioide Hayata. The cloned genes were sequenced and their amino acid sequences predicted.

我们自资料库中收集并分析已发表之草本和木本植物caffeoyl CoA3-O-methyltransferase序列，在具高保守性的序列中设计了一对引子，并对红桧、台湾扁柏、台湾冷杉、台湾铁杉和台湾杉进行OMT基因的钓取，同时配合RACE的策略，成功获得红桧、台湾扁柏、台湾杉的全长基因及台湾冷杉与台湾铁杉之部分基因片段。

The distribution property of combinatorial sequences is a basic problem in combinatorics. One of the most important properties is unimodality which includes unimodal, log-concave, log-convex and PF properties.

组合序列分布性质的研究是是组合数学中最原始最基本的问题之一，其中一类重要的分布性质是单峰型性质，包括单峰性、对数凹性、对数凸性和PF性质等。

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

YAA at amino acid level.

从实验室保存的一株能高效降解苯胺的不动杆菌中克隆到完整的苯胺双加氧酶基因簇，序列分析表明该基因簇包含6个完整的ORF，全序列与已报道的不动杆菌YAA的苯胺双加氧酶基因簇在氨基酸水平上有较高的同源性。

The result showed that the nucleotide and amino acid sequence of the cloned Bm86 gene shared 97% and 95.6% homologies with the data published in GenBank respectively.

测序分析表明：克隆的微小牛蜱Bm86基因序列与GenBank上登录的Bm86基因的核苷酸和氨基酸序列的同源性分别为97%和95.6%。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。