序列性

we carry on logistics demand forecast by taking time-series model and explain its application to logistics demand forecast by justment of data stationary, stead, standardization, modeling, discernment, making steps, parameter estimate and examination to predict, error and calculation of confidential interval.

本文采用时间序列模型进行物流需求预测，用实例从数据平稳性的判断，平稳化，标准化，建模，经模型的识别、定阶、参数估计和检验，到预测及误差和置信区间的计算，详细地说明了时间序列模型在物流需求预测中是如何应用的。

Objective To solve the difficulties of identification of Sarcosaphagous flies such as Lucilia sericata and Lucilia cuprina which could not be identified by analyzing the 278 bp and 635 bp regions of the gene encoding for cytochrome oxidase subunitⅠand Ⅱ in mtDNA.

目的通过mtDNA上16SrDNA中551bp基因序列分析，解决依据COI和COII上278bp和635bp基因序列难以鉴别丝光绿蝇、铜绿蝇种类的难题，为法医学嗜尸性苍蝇种类的鉴别提供依据。

Results There was no significant gene difference between adults and larvae. CO Ⅱ gene sequences could he used to identify Boettcherisca peregrina, Aldrichina grahami and Lucilia illustris but they could not distinguish Lucilia cuprina from the Lucilia sericata because of their close evolutionary distance and single nucleotide polymorphisms in aldrichina grahami and Lucilia illustris populations were found.

结果 成虫与幼虫基因差异不明显，COⅡ基因序列可以对棕尾别麻蝇、巨尾阿丽蝇和亮绿蝇进行鉴定，铜绿蝇与丝光绿蝇进化距离较近，COⅡ序列不能将他们区分开，同时还发现巨尾阿丽蝇和亮绿蝇存在种群单核苷酸多态性。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Two virus samples were collected from Malvastrum coromandelianum and these samples were tested by PCR. The results showed that a specific band about 500 bp was amplified from the samples Fz1 and Fs1 with degenerate primers PA and PB.

PCR产物测序进行序列比较发现：2组赛葵样本上病毒之间的差异极小，核苷酸序列同源性大约为96％，因此推测：这两组赛葵样本可能是由(来源：Af3BC7bab论文网www.abclunwen.com)同一种病毒侵染。

RbcL gene was obtained by polymerase chain reaction and cloned into pDM19-T vector. The positive clone identified by PCR was sequenced. The nucleotide sequence was 1266 bp long, including an ORF of 1031bp, encoding a putative 343 amino acids. The deduced amino acid sequence had a high identity above 94.13% with that of tobacco, spinach, sweet potato, corn, Arabidopsis thaliana, Oryza sativa, Vitis vinifera, Marchantia polymorpha and Citrus paradise.

阳性克隆鉴定后进行测序，序列分析结果表明：该基因片断长为1266 bp，包括1031 bp的编码区序列，编码343个氨基酸；其编码区氨基酸与烟草、菠菜、玉米、甘薯、拟南芥、水稻、葡萄、地钱和葡萄柚等9个物种的同源性94.13%以上，并构建了它们间的亲缘关系树。

Comparative studies of the thermophilic and mesophilic enzymes showed that they had high homologous sequence, high similar 3D structure and catalytic mechanism.

嗜热酶与对应的中温酶序列具有高度的序列同源性、高度相似的三级结构和催化机制。

Objective To detect and compare the COI gene sequences of Necator americanus collected from the provinces of Sichuan, Hainan, Yunnan, Hubei and Jiangsu, and to analyze the genetic diversity of the geographic isolates.

目的测定和比较四川、海南、云南、湖北、江苏五省美洲钩虫细胞色素氧化酶Ⅰ基因序列，分析基因序列的多态性。

Two of the six clones had the same DNA sequence,so there are five different antigenic epitopes in the six clones;the Blast analysis showed that there were no sequence homology between the five epitopes and the known antigen of paragonimus.

上述6个克隆有2个克隆的DNA序列完全相同，即6个克隆包含了5种不同的抗原表位；Blast分析表明5个表位与已知的肺吸虫抗原表位无DNA序列的同源性。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。