序列性

Therefore the nonrandom distribution of 3′ end sequences of effectual primers in tomato RAPD reflected the sequence-specific structure may occur in tomato genome at flank of polymorphic regions. It is probably an attribute of tomato genomic sequence and is common in Lycopersicon.

RAPD引物3′端序列的非随机分布，反映了番茄基因组内的多态性区段侧翼具有序列的特异性，这种特异性结构应是番茄基因组序列的一种属性，这种属性为番茄属基因组所共有。

Firstly, the key frames were extracted from the video sequence. Then, the features detected between adjacent key frames were guided and matched by motion displacement, which was calculated by the optical flow field. With the RANSAC robust estimation and cascade of homography matrix, the homography matrix of the adjacent key frame and the nonadjacent key frame was got. Finally, the seamless mosaic was rendered by image blending.

首先在同一镜头下的视频序列中提取若干关键帧，利用关键帧拼接表示序列的拼接；其次利用光流场算法计算出的运动位移量引导相邻关键帧间特征点的匹配，并结合随机抽样一致性鲁棒估计算法和单映矩阵的级联性，得到相邻关键帧、非相邻关键帧间的对应矩阵；最后通过融合实现了序列的无缝拼接。

The nucleotide sequences of the PAP gene and PAPⅡ gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPⅡ gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99. 8% were observed respectively. They both had one open reading frame . Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料，提取其总RNA、反转录，用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物，分别对反转录产物进行PCR扩增，将扩增的目的片段进行序列分析，结果扩增出来的PAP基因与已经报道的序列完全相同，扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8％，而且它们都各自编码一个开放阅读框架，可以说美洲商陆根中肯定存在PAP和PAPⅡ基因，并已经转录。

The nucleotide sequences of the PAP gene and PAPII gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPII gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99.8% were observed respectively. They both had one open reading frame. Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料，提取其总RNA、反转录，用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物，分别对反转录产物进行PCR扩增，将扩增的目的片段进行序列分析，结果扩增出来的PAP基因与已经报道的序列完全相同，扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8％，而且它们都各自编码一个开放阅读框架，可以说美洲商陆根中肯定存在PAP和PAPⅡ基因，并已经转录。

A polygenetic tree, constructed using the nucleotides sequences coding for Der f 2 reported from different regions or countries showed that our sequence was clustered with the strains from Reinbek and Guangzhou.

同源性、相似性、序列比对及分子进化分析提示，该序列与中国广州和德国Reinbek报道的序列亲缘关系较近。

A total of 23.5％ of these EST-quality sequences were putatively identified sequence of Schistosorma mansoni or other organisms, and 57.6％ which had no matches in the database were classified as unknown sequence. Some interesting new genes were found.

曼氏血吸虫或其他生物的同源序列占有EST价值的序列的23.5％，未知序列占57.6％，通过同源性分析，发现了一些令人感兴趣的新基因。

The pheromone receptor Lercb1-B1 of L. edodes had the homology with these pheromone receptors from Coprinus cinereus and Schizophyllum commune based on alignment, and was seven transmembrane protein following the analysis using the SOSUI software.

经序列比对分析，香菇的信息素受体LErcb1-B1序列与灰盖鬼伞和裂褶菌的信息素受体之间具有同源性，经SOSUI软件分析该序列具有7次跨膜结构特征。

Results of sequence and homology analysis show that MD 1 has 97% similarity with mark in maize chloroplast genome,a gene encoding RNA maturase involved in group Ⅱ intron splicing of RNA transcript;MD2 has 99% similarity with the gene serine/threonine phosphorylase 2C in Sporobolus stapfianus;and MD3 has 99% similarity with rice the gene encoding metacaspase,an arginine/lysine-specific cysteine protease.

序列分析和同源性比对表明，MD1与编码成熟酶的玉米叶绿体基因matK有97%的相似性，MD2与极端耐旱植物Sporobolus stapfianus编码丝氨酸/苏氨酸蛋白磷酸酶的PP2C基因有99%的相似性，MD3与属精氨酸/赖氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase酶基因有99%的相似性。根据MD2片段序列，结合电子克隆和RT-PCR方法，克隆出一条1731 bp的全长cDNA序列，它编码388个氨基酸。

We tested whether inhibitory GABAergic synaptic transmission regulates Xenopus optic tectal cell dendritic arbor development in vivo by expressing a peptide corresponding to an intracellular loop ofthe gamma2 subunit of type A GABA receptors GABA(AR, which is required to anchor GABA receptors to the postsynaptic scaffold.

我们想证明如下设想：抑制性GABA能突触传递是否能调节早蟾视顶盖细胞的树突发育。因为GABA受体的gamma2亚基有一段细胞内的跨膜端序列，这段序列对锚定GABAA受体到突触后膜是必须的，所以我们的主要方法是设计了一段特异性的争对细胞内段的序列，这样就特异性的阻断了单个细胞GABA能的突触传递。

Secondly, the genomic DNA is extracted from Zostera marina L. and then amplified by PCR with two oligonucleotide primers synthesized to obtain the partial nucleotide sequences of the Na~+/H~+ antiporter gene. The Na~+/H~+ antiporter gene clone was obtained after a series of manipulation: purificatioin of PCR product, ligation and transformation of recombinant plasmids, blue-white selection and so on. DNA sequence analysis shows that its DNA sequence is highly homologous with the genomic DNA sequence of E.

其次，通过提取大叶藻基因组DNA，根据设计的简并性引物进行PCR扩增，纯化PCR产物，连接，重组质粒转化，蓝白斑筛选等操作，并测序，提交测定序列到NCBI进行BLASTN，结果表明：该序列与大肠杆菌的基因组序列相似性较高，而与其它物种的Na~+/H~+逆向转运蛋白基因没有相似性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。