序列性

In this paper, the cDNA sequence from Ampullaria crossean stomach named Dy1300 was obtained by RT-PCR firstly and cloned into vector pMD 18-T. Its ORF named SXYN contains 882bp nucleotide encoding a 294 amino-acid protein. The percentage of similarity of SXYN with the reported cellulase EGX gene sequence from Ampullaria crossean was 89.8%.

本研究首先通过RT-PCR的方法从福寿螺胃中扩增出新的纤维素酶cDNA序列―Dy1300，并与克隆载体pMD 18-T相连接，通过对其全序列的分析，确定其开放阅读框ORF长度为882bp，命名为SXYN(GenBank， AY941794)，推定的氨基酸序列含294个氨基酸，与已报道的福寿螺纤维素酶C端基因的同源性达89.8%。

Analyzing by blastn program of NCBI, the fragment includes: 30bp 12srRNA partial sequence, 71bp tRNA-Val complete sequence and 913bp 16srRNA partial sequence, and the homolog is more than 95 % compared with other Camelidae animals.

PCR条件优化后成功扩增出长度为1014 bp的DNA片段，blastn分析显示，该片断与骆驼科动物的同源性高于95%，所获得片断包括30 bp的12srRNA基因部分序列，71bp的tRNA-Val基因全序列和913bp的16srRNA基因部分序列。

The genetic DNA was extracted from the blood of 6 A. forsteri which sex was unknown. The PCR amplified fragments with primers P2/P8 were cloned in T-Vector. Taking homologous sequence of Circaetus gallicus as reference, the CHD gene sequence was compared to the homologues in GenBank to identify its sex.

方法］采用苯酚：氯仿抽提法提取6只未知性别的帝企鹅血液中的基因组DNA，运用P2/P8引物扩增CHD基因片段，将PCR产物克隆到T-Vector，利用NCBI的Blast程序，以短趾鹰同源序列为比对参照，将帝企鹅CHD基因片段序列与GenBank中的基因片段序列进行同源性比较分析，鉴定帝企鹅的性别。

aim: to generate random sequences that can fit the anticipated probability distribution based on the thinking of mc, methods: we establish a method in the ide of visual studio by the way of linear congruential method to generate random sequences.we also test the homogeneity and independence by the chi-sqare goodness of fit test and self-correlation test.

v+\$oXb；L^7h_0 目的：基于mc法生成符合预期概率分布的随机序列，以便应用在以后的仿真系统中。方法：应用线性同余法在visual studio环境中建立随机模型模拟随机序列生成。并采用χ2拟合优度检验法和自相关检验法检验生成的随机序列的均匀性、独立性。

The induced amino acid sequence identity with other cucurbitaceous ribosome-inactivating proteins was up to 68.90%, with TCS was 71.25%, with Gelonin was 71.25%, with Saporin was 66.45%. The amino acid sequences of 90 to 220 were conservative.

BC-MAP30和YT-MAP30基因的推导氨基酸序列与其他葫芦科植物的Ⅰ型核糖体失活蛋白以及天花粉毒蛋白、多花白树毒蛋白、肥皂草毒素蛋白的氨基酸序列同源性分别为68.90％、82.75％、71.25％和66.45％，且其第90～220位氨基酸序列较为保守。

Cuspidate with 33％ identity and 50％ similarity to taxadien-5α-ol-O-acetyltransferase, 34％ identity and 52％ similarity to 10-deacetylbaceatin Ⅲ-10-O-acetyltransferase, and 33％ identity and 49％ similarity to taxane 2α-O-benzoyltransferase, respectively, moreover, it contained the conserved HXXXDG motif that is found in other transacylases, this sequence element has been suggested to function in acyl group transfer from acyl CoA to the substrate alcohol.

No.AF448807）在氨基酸水平与大部分植物来源的酰基转移酶具有较大的同源性，其中与东北红豆杉紫杉二烯-5α-醇-乙酰转移酶的一致性达33％、相似性达50％，与东北红豆杉紫杉10-去乙酰巴卡亭Ⅲ-10-0-乙酰转移酶的一致性达34％、相似性达52％，与东北红豆杉紫杉二烯2α-0-苯甲酰转移酶的一致性达33％、相似性达49％，并且在TS4的氨基酸序列中还发现酰基转移酶家族成员特有保守序列HXXXDG，这个序列单元被认为在将酰基CoA转移至醇类底物的过程中发挥作用。

The key research progresses and results that we accessed by the project were as follows: 1 A pair of degenerated primers were designed in the conserved domain which based on the amino acid sequence of HAK in Arabidopsis thaliana. The 500 bp fragment of HAK were amplified from the roots of Puccinellia tenuiflora by RT-PCR. The full sequence of HAK were obtained by 5' and 3' RACE methods and were determined and analyzed.

本项目主要取得了以下研究进展和成果：（1）根据已有HAK转运蛋白氨基酸序列在保守区域设计简并PCR引物，用反转录PCR方法，扩增出500bp的小花碱茅根HAK片段，通过5''和3''RACE的方法获取了该HAK的全长序列，完成了测序和序列同源性分析。

Sequence comparison showed that the deduced amino acid of the partial fragment of catalase gene from F. chinensis shares 95% and 73% identity with that of Macrobrachium rosenbergii and Haliotis discus, respectively.

多序列比对结果发现目前所得中国明对虾Catalase基因部分片段的推导氨基酸序列与罗氏沼虾和皱纹盘鲍Catalase氨基酸序列的同源性分别达到95%和73%。

According to the results of Blastn and Blastp in GenBank, it shares 72%,70%,60%,54% nucleotide sequence identity and 79%,73%,65%,57% amino acid sequence identity with Hordeum vulgare lipid transfer protein gene LTP7a2b and LTPcw-19, Zea mays ltp and Brassica napus ltp separately.

将核苷酸序列和推断的氨基酸序列在GenBank中进行比较，发现Siltp与大麦脂转移蛋白LTP7a2b和LTPcw-19，玉米脂转移蛋白，欧洲油菜的脂转移蛋白基因的核苷酸序列的同源性分别为72%，70%，60%，54

Lamblia SUCH/89/BTMRI/2(C2) variant-specific surface antigen gene to that of sequence（TSA417） published in GenBank was 99% both at the nueleotide and the amino acid levels.

该表面变异抗原基因的核苷酸序列及推导的氨基酸序列同GenBank中Gillin发表的TSA417序列的同源性均高达99％。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。