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Objective To identify gene mutation of a epidermolysis bullosa pruriginosa family. Methods Polymerase chain reaction, DNA squencing, multiplex PCR using allele-specific oligonucleotide primers.

采用聚合酶链反应，DNA直接测序以及等位基因特异引物的多重PCR方法，对一个痒疹样大疱性表皮松解症家系40人（其中患者23例）进行基因突变情况检测。

Methods The LPL cDNA was isolated from the human epiploon adipose tissue by means of RT-PCR. The LPL cDNA was ligated into the pcDNA3.1Zeo. The recombinant pcDNA3.1Zeo-LPL cDNA was identified by endonucleases, PCR and DNA sequencing. COS-1 cells were transfected with the recombinant LPL gene plasmid using Lipofectamine 20001M The LPL mass in cells and the culture medium were determined by a Markit-M LPL Kit. Spectrophotometry was used to measure the LPL activity.

采用RT-PCR法从人大网膜脂肪组织获取LPL cDNA基因，以pcDNA3.1Zeo质粒作为载体，运用基因克隆技术构建野生型人LPL cDNA重组质粒，限制性内切酶酶切、PCR以及双向测序鉴定重组质粒；运用Lipofectamine 2000将重组pcDNA3.1 Zeo-LPL cDNA质粒导入COS-1细胞，RT-PCR法检测LPL mRNA，ELISA法和比色法分别检测细胞裂解液及细胞培养基中LPL浓度及活性。

This established reverse genetics system provides a powerful tool for the research of the P gene in this study.1.Full-length genome analysis of Newcastle diseases virus strains belonging to genotypeⅢandⅨreveals the common origin of recent epizootic viruses.

基因Ⅲ型和Ⅳ型新城疫病毒全基因组测序及其在新城疫进化史中的重要地位分析根据EMBL/GenBank中已发表的基因Ⅲ型NDV全基因和基因Ⅸ型NDV基因片段设计一套全基因引物，将NDV全基因分为10个相互重叠的片段进行RT-PCR扩增。

The conceptions of the superiority, inferiority and equipollence position degrees of the interval grey number were defined, and it was proved that the sum of three position degrees for a certain interval grey number related to another grey number is 1, and that the relation set of the position degrees is a whole order set of the interval grey numbers.

运用灰色系统思想和系统工程的理论，揭示了人们在灰信息条件下的博弈心理与博弈决策规则；提出了区间灰数的优势、均势和劣势的概念；证明了与某一区间灰数相对的另一区间的各种灰数势之和为1，且灰数势大小关系的集合是一个全序集。

A preorder that is symmetric is an equivalence relation .

满足对称性的预序关系称为等价关系。

The erasion of the mark likely was crucial in the process of the genome epigenetic reprogramming.

总之，TBP在有丝分裂中期染色体上的残留可能是分化细胞特有的细胞记忆标记；这一标记在核质重组胚的核重序过程中被去除。

After that, the author focus his attention on the pure sequential confidence for the error variance in linear models.

紧接着，作者把注意力转移到线性模型误差方差的纯序贯区间估计。

On the basis of the previous work, we make further study on the mechanism of the denucleation process, especially on the role of different cytoskeletal elements in the denucleation process. To clone and sequence a gene related to terminal differentiation of erythroblast, and construction of two cDNA libraries of erythroblast differentiation. The results of our study are as follows

本文在我室前期工作的基础上，对红系细胞终末分化中的排核机理作了进一步的探讨，克隆、测序并表达了一个与红系细胞终末分化相关的基因，另外，还成功地构建了小鼠终末分化和差减cDNA两个红系分化cDNA文库，为本室以后的基因克隆工作打下了基础。

The fourth section of the Mass is the Eucharistic Prayer.

第四部分的群众性，是圣体圣事的祷告，本节开头序言中，介绍祈祷结束与 sanctus ，然后按照中央圣体祈祷，或佳能，其中载有叙事的耶稣'学会感恩祭。

The effects of Be content on eutectoid temperature and on order-disorder transition temperature were be discussed.

本论文以真空石英胶囊熔配三种铝含量在共析成份附近之Cu-Al-Be形状记忆合金，藉由添加不同之铍含量，以探讨铍含量对合金共析温度、序化结构转变温度之影响。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。