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Based on the high-resolution sequence stratigraphy and subsidence analyses of 73 wells in the Beryl Embayment, Viking Graben, North Sea , this paper tries to describe the sedimentation and subsidence models of the Middle-Late Jurassic syn-rift phase, andto determine the timing and location of boundary fault linkage and variation of active extension directions, and their control onsedimentation and .

根据北海维京地堑Beryl淘73口井的高分辨率层序地层学分析和高分辨率沉降分析，阐明中、晚侏罗世沉积和沉降模式，确定应力场的变化及边界断层的连锁对沉积和含油气系统的控制作用。

Polymerase chain reaction is amplified and sequenced to determine the type of emm genes.

聚合酶链反应扩增并进行测序从而对emm基因进行分型确定emm基因的型别。

The steps of software development are: at first, link the database and auto-count the volume and the density of each encasement then sort descending the encasement according to the density.

软件的实现步骤为：首先连接数据库并据密度贪婪算法自动计算每件货物的体积、密度，然后将货物记录按密度降序排序。

In what follows we'll be assuming little endian ordering.

下面我们将假定小端序。

METHODS: Tum5 gene was amplified from plasmid pSPORT1Sfi by PCR technique and subcloned into the expression plasmid of lentiviral vector, pGCFU, to generate the lentiviral expression vector, pGCFUTum5. The correct Tum5 gene was confirmed by endoenzyme digestion and sequencing. Recombinant lentiviruses were produced by 293T cells following the cotransfection of pGCFU Tum5 and packaging plasmids-pHelper1.0and pHelper2.0. The resulting recombinant lentiviruses (GCFUTum5) which carried Tum5 and EGFPgene were then used to infect human umbilical vein endothelial cells.

采用PCR技术从含有tumstatin基因的质粒克隆模板 pSPORT1Sfi钓取Tum5基因，并将基因克隆到慢病毒载体表达质粒pGCFU中，构建慢病毒载体表达质粒pGCFUTum5，通过酶切、测序验证Tum5基因后，将pGCFUTum5质粒和包装质粒pHelper1.0，pHelper2.0共同转染人胚胎肾上皮细胞系293T细胞，获得携带Tum5基因和EGFP基因的重组慢病毒GCFUTum5，并转染靶细胞人脐静脉血管内皮细胞。

III For constructing the expression vector of a fusion protein and obtain a target protein with full identity on aa sequence of a natural 13- 1,3-1 ,4-glucanase, with the recombined plasmid DNA harbouring the target gene as template, the primers designed with restriction sites for both terminals and enterokinase recognition site, followed by PCR amplification, was induced to the target gene.

为构建融合蛋白表达载体和获得与天然蛋白质序列完全一致的目的蛋白，以含有目的基因的重组质粒DNA为模板，设计引物时加入两端酶切位点及肠激酶裂解位点，通过PCR扩增引入目的基因中，测序结果表明接头和读框正确。

As a useful tool, computer has been widely used in the entomological research works including insect classification.

计算机作为一种昆虫分类的工具，已广泛用于昆虫的数值分类和支序分类中。

If the entwine cost was paid, follow the text of each of the modes in the order written on the card when the spell resolves.

若是支付了打包费用，则在此咒语结算时按照牌上的内文来依序执行各模式。

The results were testified by enzymolysis and sequence scan.

酶切和测序结果均显示这两种重组质粒构建成功。

RESULTS:①Enzymolysis and nucleotide sequence analysis of recombinant plasmids showed that two VEGF-C siRNA eukaryotic expression vectors were constructed successfully.

结果：①重组质粒的酶切、核苷酸序列分析结果：经酶切和测序鉴定证实成功构建了血管内皮生长因子C小干扰RNA表达载体，建立了稳定转染细胞株。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。