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CDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique, and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1335bP的片断编码444个氨基酸残基。

Insect resistance to Bt toxin is related with the variation of cadherin-like protein gene.cDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique,and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1 335 bp的片断编码444个氨基酸残基。

With primers designed according to sequence(AY052417) of diapause pupae of oriental tobacco budworm registered on GenBank, a double-strand cDNA fragment of DH gene was obtained from RT-PCR amplification and was further cloned into pGEM-T Easy vector.

根据GenBank发表序列(AY052417)设计引物，以烟夜蛾(Helicoverpa assulta Guenée)滞育蛹为材料，利用RT-PCR技术扩增到滞育激素(diapause hormone，DH)cDNA，并通过TA克隆的方法将其克隆至pGEM-T Easy载体上，对目的基因进行测序，并利用设计的酶切位点将目的基因从T载体上切下。

It simulates the circuit breaker with a zero impedance branch, and adopts the linear network characteristic and theory, variable structure and variable parameter analyses to transfer the short-circuit current flow in the bus-bar equality into each side of circuit breaker, and deduces the sequence component model and algorithm for calculating the short-circuit current of circuit breaker when the short-circuit fault occu...

该方法将断路器作为一条阻抗为零的支路来模拟，运用线性网络特性、定理及变结构与变参数的分析方法，将母线上短路时的短路电流等值转移到断路器两侧，导出了相当于在断路器两侧分别发生短路故障时，流过断路器的短路电流的序分量模型和算法。

Aiming at the different types of basins developed in Caledonian period in the region, the sequence stratigraphy filling models of the different type basins are established.

针对研究区内在加里东期发育的不同类型盆地，建立了不同盆地类型的层序地层充填模型。

One side shows off mirror gun metal calfskin embossed with a motif evoking the facets of cut crystal.

一面镜子炫耀枪金属压花小牛皮与序引起了方方面面的水晶。

The rotation will be: Keelung - Hong Kong - Chiwan - Xiamen - Shanghai - Qingdao - Pusan - Manzanillo - Buenaventura - Callao - Iquique - Valparaiso - Lirquen - Yokohama - Keelung .

挂靠港序为：台湾基隆-香港-赤湾-厦门-上海-青岛-釜山-墨西哥曼萨尼罗港-哥伦比亚布韦纳文图拉-秘鲁卡亚俄-智利伊基克-智利瓦尔帕莱索-智利利尔昆-横滨-基隆。

Being from Wendeng City of Shandong Province, Hong Xu-Guang is a contemporary ink-colored painter and calligraphist. In 1983 he graduated from Hubei Fine Arts Institute majoring in Chinese Painting.

洪序光，山东文登人，海外归国当代彩墨画家、书法家。1983年毕业于湖北美术学院专修中国画，教授，高级美术师。

Based on this, early Cambrian in the Shandong region is divided 4 sequences.

以此为基础，将山东地区下寒武统划分为4个层序。

The study indicates that the optimum windows width decides the layering precision, interface partition with sym6 wavelet is better than with db4, canonical wavelet decomposition of conventional logging curves can well meet the demand of coenocorrelation.

研究表明，选择恰当窗宽小波决定测井曲线小波分层精度、测井数据sym6小波分解的小层界面划分效果要稍好于db4小波、常规测井曲线正则性小波分解可以很好地满足高分辨率层序地层划分要求。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。