序

The distance between a boundary point and its neighboring boundary points is used to detect the transitional points.

面对如此大量的数据如何提高数据的利用率是后序产品造型工作面临的严峻问题之一。

In Chapter 2, we use Zorn lemma and generalized order set general principle [47] to generalize classical Caristi fixed point theorem, respectively, which weaken the condition that the functional is bounded from below.

在第二章中，我们首先分别利用Zorn引理和推广的序集一般原理[47]推广了古典的Caristi不动点定理，削弱了泛函下方有界这个条件。

Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通过GenBank中的中华绒鳌蟹线粒体16S rDNA序列设计引物，利用PCR扩增方法，以beta-actin做内参，检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况，发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致，表明各模板DNA量基本一致；而在同一DNA浓度下，16S rDNA的扩增片段长度虽一致，但其产物量存在明显差异，精子16s rDNA产物量显著低于其他3种组织，其条带宽度和亮度很弱；16S rDNA扩增产物经测序分析及比对证明与已有序列完全吻合。

This study first discovered it is different in nuclear DNA between Ogura-CMS line and its maintainer in the Brassica crop.

根据测序结果设计了1对特异引物，将M6E7-700标记转化为更稳定的SCAR标记。

We designed primers according to the sequence of Genbank from Bifidobacterium breve and have cloned the putative isomerasekey gene and several fragments from the genome DNA of Lactobacillus acidophilus .

根据Genbank中短小双歧杆菌中可能的共轭化酶的序列资料，设计引物，克隆了可能的基因及几个片段并测序，其基因功能有待进一步验证。

To investigate variation of soil bacteria community in response to the change in the above-ground vegetation, soil samples were collected at three sites, i.e. bamboo groves, mixture of bamboo and broadleaf tree and broadleaf forest. To explore variation of the soil bacterial structure, 16S-rDNA-V3-fragment-base DGGE (denaturing gradient gel electrophoresis) and clone sequence BLAST technologies were applied.

为了解毛竹人侵天然林后地下土壤微生物多样性的变化，分别采集了毛竹纯林、竹阔混交林和原始天然阔叶林下的土壤样品，应用建立于165 rDNA V3区片段的变性梯度凝胶电泳和克隆测序比对来研究土壤细菌结构的变化。

Objective:to compare sequential therapy and conventional intravenous infusion treatment of the clinical effect of bronchopneumonia.

目的：比较序贯疗法和常规静脉滴注法治疗支气管肺炎的临床疗效。

Objective To analyze the effect of sequential therapy of Cefixime Granules in children with bronchopneumonia.

目的 分析头孢克肟颗粒剂序贯疗法治疗支气管肺炎的疗效。

Methods:70 cases of bronchopneumonia in children were randomly divided into sequential therapy group(n=36)and intravenous infusion group(n=34),the effects were observed.

将70例小儿支气管肺炎随机分为序贯疗法组36例和静脉滴注法组34例，观察两组疗效。

Sequential therapy with cefuroxime for children with bronchopneumonia is not only safe,valid,but more economic reasonable.

头孢呋辛序贯治疗儿童支气管肺炎不但安全、有效，而且更加经济合理。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。