序

Two pairs of primers were designed based on the sequence of ovis aries preprochymosin cDNA reported in GenBank.The preprochymosin cDNA was obtained from toltal RNA isolated from the abomasum of 5-day-old Xinong Saanen Goat by RT-PCR method and then cloned successfully in E.coli.

参照GenBank登录的绵羊凝乳酶原前体cDNA序列设计引物，以新生5d的西农萨能羊皱胃组织总RNA为模版，通过RT-PCR方法获得凝乳酶原前体cDNA，克隆测序后进行序列比对。

In our study, the omp1 gene of Chlamydophila abortus of swine was amplified by PCR and was sequenced.

本研究通过PCR扩增了猪流产嗜性衣原体omp1基因，并进行了测序鉴定。

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

When any one of the 3 phases failure, or phase sequence does not conform to the original status, the relay for phase will operate to break the safety circle and stop the lift.

三相电源当任何一相断开及三相电源相序与出厂规定不符时，相序继电器动作，断开安全电路，电梯不能运行。

It was pointed that encoding is important to DNA computing, in the implement of the DNA algorithm, the technique was used which is to safeguard 3＇ margin or 5＇ margin of DNA sequence corresponding the two points in order to find out all chains, and electrophoresis could be applied to arrange all chains and to throw off all the chains whose length exceed summation of weigh in graph, The probe with biotin to observe and register intensity of bright point to filter all paths; finally the orders of all paths can be obtained to analyze the recordation of experimentation.

得到路序。指出编码问题在DNA计算中的重要性。在算法实现过程中，用保护两端点对应的DNA片段3'端或5'端的办法得到所有的链，并用电泳的方法对链进行排序以及去掉链长大于图权值总和的链；对探针进行生物素标记并且采用观察、记录亮点强度的办法筛选出所有的路；分析实验记录得到路序。

This is also the common feature of this time.

这些题序忠实记录了他的人生，这也是中兴时期题序的一个共同特点。

Since there is a close connection between principal filters and the smallest complete semi-lattice congruences on a po-semigroups, the study of the structure of filters has attracted a number of authors. For example, Kehayopulu, Xie X.Y, Cao Y.

在偏序半群中，由于主滤子同最小完全半格同余乃至完全半格同余有着密切的联系，又主滤子在偏序半群结构的研究中起到至关重要的作用，因而对滤子结构的研究吸引了众多学者的关注。

By analyzing the conditions and patterns of the regional oil and gas bearing formations with typical crosssections, we have established the patterns of sedimentary conditions for different reserviors.

为了解决上述问题，论文大力开展区域层序地层学研究，建立起全盆地层序和体系域的等时地层格架，揭示沉积体系的时空展布和演化特征，搞清大的地质背景。

This model can describe the competing and coexistance of the antiferromagnetic order and superconducting order in high temperature superconductors.

该模型可以用来描述超导体中反铁磁序与超导序的竞争和共存。

By means of the principle,method and technology of sequence stratigraphy,The dynamic characteristics of filling and evolution of Huangxian Eogene fault basin are studied,based on the classification of high frequency sequences.

运用层序地层学原理和方法，在黄县盆地进行高频层序地层划分的基础上，对黄县早第三纪断陷盆地充填演化的动力学特征进行研究。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。