幼虫的

CDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique, and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1335bP的片断编码444个氨基酸残基。

Insect resistance to Bt toxin is related with the variation of cadherin-like protein gene.cDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique,and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1 335 bp的片断编码444个氨基酸残基。

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

It was so concluded that silkworm hemopoietic organs were related to the formation of hemolymph proteins, and due to the decrease of hemocytes caused by functional disruption of hemopoietic organs, synthesis of protein in fat body was affected and protein components in hemolymph changed.

由此认为家蚕幼虫造血器官与血淋巴中的蛋白质成分有关，造血器官的机能障碍、血球的数量减少可影响脂肪体中蛋白质的合成，从而使存在于血淋巴中的蛋白质成分发生变化。

You can see the fronds of the gall which give it its name at the edges of the picture – and the larvae within!

你可以看到其中的胆给在图片的边缘它的名字的叶片-和幼虫内！

Objective The toxicity and synergism of several commonly used insecticide mixtures,and DDVP mixed with synergists,to the larvae of DDVP-resistant Culex pipiens pallens were detected to delay the development of mosquito resistance to insecticides.

目的 为延缓抗性的发展，测定了淡色库蚊幼虫DDVP抗性品系对常用的几种杀虫剂混用后的敏感性及与增效剂氯化胡椒基丁醚(S1)、八氯二丙醚(S2)和增效胺混用的效果。

The decrease of protocatechuric acid content was significant at the concentration level of 6μL/L MeJA, but 6μL/L MeSA and feeding damage for 48h by the third instar larvae of maize stalk borer had an opposite effect.

原儿茶酸的含量随着MeJA处理浓度的增大逐渐减少，6μL/L的MeJA使其显著降低，而6μL/L 的MeSA和玉米螟3龄幼虫处理48h则使其含量升高。

In this research, leaves of maize grown on soil pots and vermiculite pots were treated with jasmonate methyl ester, salicylic methyl ester and third instar larvae of the maize stalk borer and were assessed for the effect on the contents of DIMBOA and phenolic acid, and the expression of defense genes in leaves and roots, the content and species of root exudates and the diversity of rhizosphere bacteria community.

本文通过土壤盆栽、蛭石盆栽实验，用化学分析和分子生物学方法研究不同浓度茉莉酸甲酯、水杨酸甲酯及玉米螟3龄幼虫处理玉米（粤农9号）叶片对叶片和根中丁布、酚酸及防御基因的表达量的影响；且探讨处理之后根系分泌物的种类和组分及土壤细菌群落多样性的变化。

After that eating larva grow up and begin moving to fight with the boss-creature.You can collect red spheres by seeking it on the level mountains or by killing red-white worms, produced by Boss-creature.

之后，吃的幼虫长大，并开始移动，以争取与老板- creature.you可以收集红色的领域，寻求它的水平对山区或杀害红白蠕虫，所产生的老板-动物。

For example, the larva or pupae of a beetle genus of the Northern Kalahari Desert is used to create a slow-acting poison that can be quite useful when hunting.

例如，在喀拉哈里沙漠北部生存的甲虫类昆虫的幼虫或蛹被用来制作一种延缓行动的毒药，在打猎的时候极为有用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。