幼虫的

In low age larva is filled period the effect of prevention and cure to garlic root maggot undertakes different drug experiment and be investigated, the result makes clear: 27 years garlic root maggot produces 3 generation in Jiangsu, generation of live through the winter is imaginal in March the last ten-day of a month fills hair; the middle ten days of a month on April fills generation larva hair, the middle ten days of a month saw in May aurelian, the last ten-day of a month was; of fastigium of generation imago ascend to heaven and become immortal in May the 2nd generation is larval the first ten days of a month was filled to the middle ten days of a month in June hair, the last ten-day of a month came in June in September the last ten-day of a month with aurelian more summer, 2 generation are imaginal at; of the ascend to heaven and become immortal at the beginning of October coming by September the 3rd acting larval fastigium is on October the middle ten days of a month, the first ten days of a month is larval in November in succession pupate live through the winter, immediately following in time year of spring (the middle ten days of a month on April) begin emergence.

在低龄幼虫盛期就不同药剂对大蒜根蛆的防治效果进行试验和调查，结果表明：2007年大蒜根蛆在江苏发生3代，越冬代成虫于3月下旬盛发；第一代幼虫4月上中旬盛发，5月中旬见蛹，5月下旬为一代成虫羽化高峰期；第二代幼虫6月上旬至中旬盛发，6月下旬至9月下旬以蛹越夏，二代成虫于9月底至10月初羽化；第三代幼虫高峰期在10月上中旬，11月上旬幼虫陆续化蛹越冬，翌年春季(4月上中旬)开始羽化。

The snail was found to have an indirect developmental type in the early development. The fertilized eggs were oval-shaped and discoidal cleavage. Embryonic development stages were divided into cleavage, blastula, gastrulae, trochophore, and intra-membrane veliger. Larval development stages included veliger, late veliger and crawling larvae metamorphosed to juvenile.

结果表明：黄口荔枝螺卵囊高度平均为7.33 mm，卵粒数量平均为165粒；黄口荔枝螺的早期发育属间接发生型，受精卵呈椭圆形，卵裂为盘状卵裂；在卵囊内，胚胎发育包括卵裂期、囊胚期、原肠胚、膜内担轮幼虫、膜内面盘幼虫；幼虫发育包括面盘幼虫、后期面盘幼虫和匍匐幼虫。

Dominant species were comprised of mainly micro- and meso-zooplankton species and planktonic larvae of benthos such as Parvocalanus crassirostris, Cirripedia nauplius, Oithona attenuata, Acrocalanus gibber, Nauplius, Bivalvia veliger, Copepodite, Oithona simplex and so on.

优势种主要是中小型种类和底栖动物的幼虫，如强额孔雀哲水蚤、蔓足类无节幼虫、细长腹剑水蚤、驼背隆哲水蚤、无节幼虫、双壳类面盘幼虫、桡足幼体、简长腹剑水蚤等。

Plutella xylostella larvae of a strain highly resistant to spinosad were used as hosts to rear the parasitoid Cotesia plutellae. When the parasitoids had developed to the stage of early instar larva, the host larvae were treated with 50 mg/L spinosad on the terga by topical application and then fed with cabbage leaves treated with 50 mg/L spinosad to allow the host larvae and the parasitoid larvae to develop. Three days after treatment, the parasitoid larvae inside the host were collected by dissecting the host larvae. The body fluid of P. xylostella and the bodies of parasitoid larvae were then analyzed with HPLC. Both of the active components, spinosyn A and spinosyn D, of spinosad were detected in the body fluid of host larvae and in the bodies of parasitoid larvae, and the residue concentrations were 2.79 mg/L and 0.94 mg/L in the host and the parasitoid respectively.

以对多杀菌素高抗的小菜蛾Plutella xylostella幼虫作为菜蛾绒茧蜂Cotesia plutellae的寄主，待绒茧蜂发育到1龄幼虫时，将浓度为50 mg/L的多杀菌素点滴到寄主幼虫背板上，随后让寄主幼虫取食经50 mg/L多杀菌素处理过的甘蓝叶片，寄主幼虫和其体内的蜂幼虫再发育3天后，将寄主幼虫解剖取出蜂幼虫，用高效液相色谱法对经多杀菌素处理的小菜蛾幼虫体液以及绒茧蜂幼虫匀浆液进行检测，结果多杀菌素的2个活性成分spinosyn A和spinosyn D均被检测到，两者的多杀菌素残留浓度分别是2.79 mg/L和0.94 mg/L。

In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象，分别对文蛤幼虫发育过程中贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程中细胞凋亡相关的caspase三个基因进行了克隆，分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征，解析了其可能的功能，并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响，进行了目标蛋白的功能验证，详述如下：研究结果显示，在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水中培养，发育成无壳的畸形，随着人工海水中铁离子添加浓度的升高，幼虫长出壳状组织接近正常状态；而发育到L1期幼虫放入不含铁离子的人工海水中培养却可以发育出正常的壳，推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

Our study displayed that HmgD overexpression mutant died as late third instar larvae and malformed pupae during the larvae-prepupa transition,suggests that overexpression of HmgD has effects rather on the third instar larvae stage than on the first and second instar larvae from the mutant could not pupate.

结果表明，HmgD广泛过量表达突变体幼虫在一龄和二龄期发育正常，而在三龄幼虫化蛹时发育异常，发育停滞在三龄幼虫末期至预蛹的阶段，即HmgD过量表达的幼虫不能正常化蛹。

More simply, that is, Vespertilionidae bat moths for many types of other offspring, spawning in the soil, after the eggs into larvae, previously, the Cordyceps fungus penetrated larvae absorb the material as the survival of larvae of the nutritional conditions, and the larvae continue to breeding, resulting in larvae filled with mycelium, in the coming year 5-7 when the weather gets warmer months, since the larvae grow yellow or light brown head and seat of the bacteria, the growth was out of the ground after the grass stalk-like, the result we usually see caterpillar fungus.

通俗地讲，就是蝙蝠科许多种别的蝙蝠蛾为繁衍后代，产卵于土壤中，卵之后转变为幼虫，在此前后，冬虫夏草菌侵入幼虫体内，吸收幼虫体内的物质作为生存的营养条件，并在幼虫体内不断繁殖，致使幼虫体内充满菌丝，在来年的5－7月天气转暖时，自幼虫头部长出黄或浅褐色的菌座，生长后冒出地面呈草梗状，就形成我们平时见到的冬虫夏草。

The contents of valine and leucine of the nematode are obvious higher than that of others in different kinds of amino acid of the nematode. The analysis of irritant factors playing a important role on the production of predacious organs showed that live nematode larva or its lytic solution can cause the production of predacious organs of the strain A1, but the latter produced less than the former .The live larva of the third stage produced more predacious organs than one of the first and the second stage , so the number of predacious organs that are produced by the live larva of first and the second the stage are less than the live larva of the third stage .

刺激捕食器产生的因素在线虫体来说：活的线虫幼虫和其裂解液可以刺激捕食线虫性真菌—少孢节丛孢菌菌株产生捕食线虫性结构——捕食器；但后者刺激产生的捕食器数量较少；有活力的第三期幼虫与第一、二期幼虫相比较，可以刺激菌株产生较多的捕食器；但少孢节丛孢菌对第一、二期幼虫作用速度快；与第三期幼虫相比，第一、二期幼虫易被捕获致死。

The third-stage larvae were developed to fourth-stage larvae (the most optimal develop rate was 41%) when cultured in defined complete medium, further, cultured in defined incomplete medium, was examined no develop and a poorly survival rate. When the third-stage larvae were cultured in the defined complete medium under 37℃ and 5% CO2 in air, the larvae were began to develop to the fourth-stage larvae in cultured for 30 days, being enclosed within the sheaths of the third molts of life cycle.

广东住血线虫的第三期幼虫以MEM medium添加胺基酸、脂肪酸及碳水化合物的培养基在37℃含有5％ CO2的无菌培养箱中，培养至第21天已发育至第三期幼虫中期的阶段，第24天已发育至第三期幼虫末期的阶段，第30天开始出现带鞘的第四期幼虫，第36天则观察到虫体明显变长并蜕去外鞘及第三期幼虫特有的眼点消失的第四期幼虫。

The results showed that the antlions fed on M. domestica larvae obtained significantly higher body weight, pupal weight, relative growth rate and pupation percentage, and significantly shortened larval duration when compared with the antlions fed on S. litura and S. exigu larvae. When feeding on C. cephalonica larvae, antlions had similar body weight, pupal weight and pupation percentage, but significantly decreased relative growth rate and prolonged larval duration, than those fed on M. domestica larvae.

结果显示，用家蝇幼虫饲养的蚁狮，其体重增长、相对生长率、化蛹率、蛹重均显著高于用斜纹夜蛾和甜菜夜蛾幼虫饲养的蚁狮，而幼虫历期则比斜纹夜蛾和甜菜夜蛾幼虫饲养的短；用米蛾幼虫饲养，虽然蚁狮体重增长、化蛹率和蛹重与用家蝇幼虫饲养的差异不显著，但其相对生长率却显著低于用家蝇幼虫饲养的蚁狮，幼虫历期也比用家蝇幼虫饲养的明显延长。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。