幼虫

The activities of carboxylesterase、glutathion-S-transferase and acetylcholinesterase per insect increase greatly and the properities of esterase and acetylcholinesterase change also throughout larval development from 1-day-old to 4th instar. The affinity for Asch and the sensitivity to insecticide of acetylcholinesterase in 3rd instar larvae are significantly lower than that in 4-day-old larvae.

幼虫从1日龄生长到4龄时，单头幼虫的羧酸酯酶、谷胱甘肽转移酶、乙酰胆碱酯酶活力急剧增长，4龄幼虫相对于1日龄幼虫，HJ-R、HD-S上述3种酶系的活力分别增长了148、172.6、65.6倍和203、60.9、77.4倍；生长过程中，全酯酶、乙酰胆碱酯酶的性质也发生变异，特别是从4日龄生长到3龄时，乙酰胆碱酯酶的米氏常数、双分子速率常数均发生明显的改变，3龄幼虫乙酰胆碱酯酶对底物的亲和力以及对杀虫剂的敏感性显著低于4日龄幼虫。

The snail was found to have an indirect developmental type in the early development. The fertilized eggs were oval-shaped and discoidal cleavage. Embryonic development stages were divided into cleavage, blastula, gastrulae, trochophore, and intra-membrane veliger. Larval development stages included veliger, late veliger and crawling larvae metamorphosed to juvenile.

结果表明：黄口荔枝螺卵囊高度平均为7.33 mm，卵粒数量平均为165粒；黄口荔枝螺的早期发育属间接发生型，受精卵呈椭圆形，卵裂为盘状卵裂；在卵囊内，胚胎发育包括卵裂期、囊胚期、原肠胚、膜内担轮幼虫、膜内面盘幼虫；幼虫发育包括面盘幼虫、后期面盘幼虫和匍匐幼虫。

The ecdysis index of controlled larvae was 3.323 by 30d, while 2.933-3.038 for the treated. The transgenic Betula platyphylla could reduce the feeding of larvae. With the increase of the time of feeding, the difference of food taken between the controlled and treated became larger. The food of controlled larvae taken was 0.105293-0.13750 grams than that of the treated by 3 d. and 0.529094-0.64103 grams by 21d.

转基因植株能够降低幼虫的食叶量，3天时对照幼虫的食叶量比处理幼虫的食叶量多0.105293～0.137504克；21天时对照幼虫食叶量比处理幼虫食叶量多0.529094～0.64103克，随取食时间的延长，对照幼虫的食叶量与处理幼虫的食叶量差别更大，转基因植株的抑制作用更明显。

Itimes of that of controlled larvae. The mortal peaks of larvae wasl-7d and 29-35 d, in which most of treated larvae was killed. The transgenic Betula platyphylla could reduce the ecdysis index of larvae.

转基因植株能够延迟幼虫蜕皮，降低幼虫蜕皮指数，实验30天时对照幼虫蜕皮指数是3.323；处理幼虫蜕皮指数在2.933～3.038之间，并且处理幼虫无一能够完成幼虫期达到化蛹。

Plutella xylostella larvae of a strain highly resistant to spinosad were used as hosts to rear the parasitoid Cotesia plutellae. When the parasitoids had developed to the stage of early instar larva, the host larvae were treated with 50 mg/L spinosad on the terga by topical application and then fed with cabbage leaves treated with 50 mg/L spinosad to allow the host larvae and the parasitoid larvae to develop. Three days after treatment, the parasitoid larvae inside the host were collected by dissecting the host larvae. The body fluid of P. xylostella and the bodies of parasitoid larvae were then analyzed with HPLC. Both of the active components, spinosyn A and spinosyn D, of spinosad were detected in the body fluid of host larvae and in the bodies of parasitoid larvae, and the residue concentrations were 2.79 mg/L and 0.94 mg/L in the host and the parasitoid respectively.

以对多杀菌素高抗的小菜蛾Plutella xylostella幼虫作为菜蛾绒茧蜂Cotesia plutellae的寄主，待绒茧蜂发育到1龄幼虫时，将浓度为50 mg/L的多杀菌素点滴到寄主幼虫背板上，随后让寄主幼虫取食经50 mg/L多杀菌素处理过的甘蓝叶片，寄主幼虫和其体内的蜂幼虫再发育3天后，将寄主幼虫解剖取出蜂幼虫，用高效液相色谱法对经多杀菌素处理的小菜蛾幼虫体液以及绒茧蜂幼虫匀浆液进行检测，结果多杀菌素的2个活性成分spinosyn A和spinosyn D均被检测到，两者的多杀菌素残留浓度分别是2.79 mg/L和0.94 mg/L。

In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象，分别对文蛤幼虫发育过程中贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程中细胞凋亡相关的caspase三个基因进行了克隆，分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征，解析了其可能的功能，并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响，进行了目标蛋白的功能验证，详述如下：研究结果显示，在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水中培养，发育成无壳的畸形，随着人工海水中铁离子添加浓度的升高，幼虫长出壳状组织接近正常状态；而发育到L1期幼虫放入不含铁离子的人工海水中培养却可以发育出正常的壳，推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

The limbless没有肢体的 aquatic 在水中生活的larva 幼虫of a frog or toad, having gills and a long flat tail.

蝌蚪：一只青蛙或蟾蜍的没有肢体的在水中生活的幼虫，生有鳃和一条长而扁平的尾巴。

A cDNA segment was isolated from M. meretrix larvae. The results of caspase in situ detection in larval developmental stages revealed that there were activated caspase from L1 to L3, indicating caspase is involved in the whole developmental process. The main apoptosis area was found in velum at L3 before metamorphosis, indicating the degradation of velum might be the process of apoptosis. The larvae before metamorphosis were treated by caspase specific inhibitor to analyze the role of caspase in this process. The results indicated that caspase have function metamorphosis.

根据caspase在不同物种中的保守区域设计简并引物，在文蛤幼虫中扩增到cDNA序列片段；检测有活性的caspase在文蛤幼虫各发育时期的分布部位，发现其在L1至L3期幼虫中都有分布，说明整个幼虫形态变化过程中都有caspase的参与；细胞凋亡检测结果显示，幼虫主要发生细胞凋亡的部位在L3期幼虫的面盘，即变态过程中要退化的器官，说明细胞凋亡可能是文蛤幼虫变态过程中面盘退化的主要机制；用caspase特异性抑制剂处理变态前幼虫，发现幼虫变态率下降，初步验证了caspase在文蛤幼虫变态过程中的作用。

More simply, that is, Vespertilionidae bat moths for many types of other offspring, spawning in the soil, after the eggs into larvae, previously, the Cordyceps fungus penetrated larvae absorb the material as the survival of larvae of the nutritional conditions, and the larvae continue to breeding, resulting in larvae filled with mycelium, in the coming year 5-7 when the weather gets warmer months, since the larvae grow yellow or light brown head and seat of the bacteria, the growth was out of the ground after the grass stalk-like, the result we usually see caterpillar fungus.

通俗地讲，就是蝙蝠科许多种别的蝙蝠蛾为繁衍后代，产卵于土壤中，卵之后转变为幼虫，在此前后，冬虫夏草菌侵入幼虫体内，吸收幼虫体内的物质作为生存的营养条件，并在幼虫体内不断繁殖，致使幼虫体内充满菌丝，在来年的5－7月天气转暖时，自幼虫头部长出黄或浅褐色的菌座，生长后冒出地面呈草梗状，就形成我们平时见到的冬虫夏草。

The results showed that the antlions fed on M. domestica larvae obtained significantly higher body weight, pupal weight, relative growth rate and pupation percentage, and significantly shortened larval duration when compared with the antlions fed on S. litura and S. exigu larvae. When feeding on C. cephalonica larvae, antlions had similar body weight, pupal weight and pupation percentage, but significantly decreased relative growth rate and prolonged larval duration, than those fed on M. domestica larvae.

结果显示，用家蝇幼虫饲养的蚁狮，其体重增长、相对生长率、化蛹率、蛹重均显著高于用斜纹夜蛾和甜菜夜蛾幼虫饲养的蚁狮，而幼虫历期则比斜纹夜蛾和甜菜夜蛾幼虫饲养的短；用米蛾幼虫饲养，虽然蚁狮体重增长、化蛹率和蛹重与用家蝇幼虫饲养的差异不显著，但其相对生长率却显著低于用家蝇幼虫饲养的蚁狮，幼虫历期也比用家蝇幼虫饲养的明显延长。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。