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幼红细胞

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Results True positive rate: immature granulocyte, hemoglobin concentration variation, microcyte, left shift, macrocyte, hypochromic erythrocyte, large platelet, blasts, anisocytosis 86.7%-100.0%, hyperchromic erythrocyte 69.2%, nucleated red blood cell, atypical lymphocyte, platelet clumps

结果 真阳性率:未成熟粒细胞、血红蛋白浓度不等、小红细胞、核左移、大红细胞、低色素性红细胞、大血小板、原幼细胞、红细胞大小不等为86.7%~100.0%,高色素性红细胞为69.2%,有核红细胞、变异淋巴细胞、血小板凝集为13.3%~32.3%;以上所有参数真阴性率为82.8%~100.0%(平均95.0%)。

On the basis of observation on the denucleation process of mammalian erythroid cells in vito and in vitro, the present study was designed to investigate the regulatory effect and its mechanism of mammalian erythroblast cytoplasmic factor on malignant phenotype and gene expression of hybrid cells formed by cellular engineering fechnique of cell hybridization between the mouse plasmocytomas SP (2/0) cell line and rat erythroblasts.

本研究选用正常大鼠中幼、晚幼成红细胞与小鼠浆细胞瘤(SP2/0)细胞进行异种细胞杂交,研究哺乳动物红细胞胞质因子对肿瘤细胞恶性调控的效应及其机制。

Using this method, we can obtain about 96% of hemogenous population of intact and viable intermediate and late erythroblasts.

从70%Percoll梯度介面(比重为1.095g/ml)可收集到纯度高达96%的中幼、晚幼成红细胞。

Because of its very low levels of folic acid, vitamin B12 is also small, so goat milk to feed those who should be added folic acid and vitamin B12, or can cause megaloblastic anemia.

由于其叶酸含量极低,维生素B12也少,故羊乳喂养者应添加叶酸和维生素B12,否则可引起巨幼红细胞性贫血。

Methods Using the new BECKMAN COULTER GEN'S Ⅱ hematology analyzer we had mensurated red blood cell and reticulocyte indices in 34 healthy subjects,20 patients with microcytosis due to iron dificiency and 19 patients with megaloblastic anemia respectively.

方法应用BECKMAN COULTER GEN'SⅡ全血细胞分析仪测定34名健康志愿者、20名缺铁性贫血和19名巨幼细胞贫血的红细胞参数和网织红细胞参数,并进行比较。

Results Erythrocyte count, hemoglobin concentration and hematocrit of all the patients with malignant digestive tumor decreased, mean corpuscular volume and mean corpuscular hemoglobin increased, arid there was inconspicuous change in total leukocyte count. The indications of megaloblastic anemia and proliferative anemia in bone marrow hemogram were found in the patients. It was common that the percentage of neutrophils was higher and that of lymphocytes was lower than that of the normal controls.

结果 消化道恶性肿瘤患者的红细胞计数、血红蛋白、红细胞压积均减少,平均红细胞体积、平均红细胞血红蛋白量增高,均表现为巨幼细胞性贫血,骨髓象呈增生性贫血改变,而白细胞总数的变化较小,白细胞分类,中性粒细胞百分比超过正常范围与淋巴细胞百分比低于正常范围者多见,血小板数异常远较血红蛋白与白细胞为少,但36例肝癌患者中28例出现血小板减少。

Neomycin on ammonia acid can lead to vitamin B12 deficiency; phenytoin, methotrexate, B ammonia pyrimidine, methyl benzyl ammonium pyrimidine and Triamterene, etc. can cause folic acid deficiency; mercaptopurine, 5 - fluoro-piperidine urine density, Ara-C, etc. can interfere with nucleic acid synthesis, these drugs can lead to megaloblastic anemia.

新霉素、对氨水杨酸可导致维生素B12缺乏;苯妥英钠、甲氨蝶呤、乙氨嘧啶、甲基苄氨嘧啶及氨苯蝶啶等可引起叶酸缺乏;巯嘌呤、5--氟尿密啶、阿糖胞苷等能够干扰核酸的合成,这些药物都可导致巨幼红细胞性贫血。

Studies on the regulation of malignant phenotype expression in a mouse plasmocytoma cell line (SP2/0) by means of cellular engineering techniques of cell hybridization between the fusion of the mouse myeloma cells with rat intermediate or late erythroblasts.

三、利用细胞杂交的细胞工程手段,首次系统地研究了大鼠中幼、晚幼成红细胞与小鼠浆细胞瘤(SP2/0)细胞融合后恶性表型的表达状态,分析自然去核前红细胞胞质对骨髓瘤细胞恶性增殖的调控作用,以及晚幼期固缩核重新被激活的可能性。

Trypan blue vital staining demonstrated that there were over 95% of the cells maintained viable after separation.

台盼兰活体染色检查表明:分离后的中幼、晚幼成红细胞保持95%以上的活性率。

In addition, Immature erythrocyte, neutrophil and direct dividing of erythrocyte could also be observed on the smears.

并发现较多未成熟的红细胞以及少量正在分裂的红细胞和晚幼嗜中性粒细胞。

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