幼体

PCR and electron microscope were used to detect the virus in the ovaries,eggs atdifferent development stages,and naupliuses of infected broodstocks of P.chinensis.Thevirus was not found in eggs just spawned or not,but in the connective tissue of ovary.Thedetected results of nauplius were positive,and virus-like organisms were found to invadethe epidermis tissue of nauplius.The virus was not detected in the yolk as well as inner andouter membrane of egg.

本文利用PCR及电镜技术，对中国对虾染病亲虾的卵巢和各期卵及其刚孵化出的无节幼体进行了病毒检测，染病亲虾刚产的卵及未产出的卵均没有发现病毒存在，在卵巢的结缔组织中检测到病毒存在，刚孵化出的无节幼体PCR检测结果呈阳性，电镜下观测到有病毒样颗粒侵染无节幼体的上层表皮组织。

The results showed that water content decreased first, then increased in the gastrula stage of embryonic development, reached the highest content at the second phrase larvae stage and then began to decrease from the third phase larva stage. The content of lipid in the dry weight increased with the embryonic development stages, but decreased at intramembranous nauplius stage, the second phase larva, and the infant stage of larvae development. The rate of lipid in wet weight decreased and the content of C20:4w6、20:5w3 and C22: 6w3 decreased.

结果显示，黑斑口虾蛄在胚胎和幼体的发育过程中，水的体积分数先降低，至原肠胚期开始升高，到第二相幼体期达到最高，之后又有所回落；脂类占组织干重的比率呈升高趋势，但分别在膜内无节幼体、第二相幼体和仔虾蛄期略有降低；脂类占组织湿重的比率呈降低趋势；花生四烯酸、EPA和DHA的体积分数都呈降低趋势。

Result The heterobacteria, vibrios and pathogenic Vibrio parahaemolyticus were the roost in fertilized eggs of shrimp rot the least in nauplius, then their number would increase with growth. During whole rearing period, both heterobacteria in larvae, vibrios in water would increase by one order of magnitude, while both vibrios in larvae and heterobacteria in water would increase by two orders of magnitude.

结果］异养菌、弧菌和致病性副溶血菌数量都是对虾受精卵中高，无节幼体中最低，而后逐渐升高在整个育苗期，对虾幼体中异养菌和养殖水体中弧菌增加1个数量级，对虾幼体中弧菌和养殖水体中异养菌均增加2个数量级。

With Mab 2A3, the first positive cells were detected at the limb bud stage of the embryos. Distinguishable positive haemocytes with a diameter of about 7μm appeared in embryonic-nauplius. At the following nauplius I stage, configuration of haemocytes became more integrated and clear. Their diameters were almost similar to those of haemocytes from adult shrimp. With Mab 1H11, the first positive granular haemocytes were identified at the stage of embryonic-nauplius which existed in clusters with positive punctate fluorescence of granules distributed around the periphery of granular haemocytes.

通过对包括囊胚期、原肠胚期、肢芽期、膜内无节幼体期和无节幼体Ⅰ期在内的中国对虾胚胎的间接免疫荧光检测，最早在肢芽期胚胎中检测到2A3阳性信号；在膜内无节幼体期，2A3阳性血细胞平均直径约7μm，聚集成簇；在无节幼体Ⅰ期，血细胞形态更加完整清晰，平均直径已与成体对虾血细胞相似。1H11阳性大颗粒细胞最早在膜内无节幼体期胚胎中出现，这些细胞同样成簇存在，在其细胞质外围可见点状荧光。

There were some differences in responses of life history characteristics to increasing temperature among the four rotifer clones.

四温度间，克隆A轮虫幼体的耐饥饿时间在15℃下最长，克隆B和C轮虫幼体的耐饥饿时间在30℃下均最短，克隆D轮虫幼体的耐饥饿时间随培养温度的升高而逐步显著地缩短。

At the mixed nutrition stage larvae lived on nutriment from yolk sac and oil globules, in addition to feeding on particulate organic detritus containing Tintinnopsis spp., also on few of trochophore and veliger of bivalve and trochophore of Polychaeta in water of the earthen ponds.

混合营养期仔鱼除依靠吸收卵黄囊和油球为营养以外，还从土池水体中摄食含有拟铃虫的颗粒有机碎屑以及少量的双壳类担轮幼体、面盘幼体和多毛类担轮幼体。

To 7 days' larve alter hatching larvae mostly lived on copepod nauplii, copepodites and trochophore of Polychaeta in water of the earthen ponds, apart from feeding on particulate organic detritus.

孵化后5~7日龄仔鱼基本上依靠摄食土池水体中的桡足类无节幼体、桡足幼体和多毛类担轮幼体，但还摄食少量的颗粒有机碎屑。

This paper analysed the amino acid composition in larval mud crab, Scylla serrata,from zoea I to megalopa, and their diets, rotifer and nauplii of Artemia. An essential amino acid index was employed to evaluate the nutritive value of the protein source in diets.

本文分析测定了锯缘青蟹幼体从刚孵化至大眼幼体各发育阶段及其饵料轮虫和卤虫无节幼体的氨基酸组成，并使用必需氨基酸指数来评价饵料蛋白质的质量。

Temperature, clone and their interaction influenced significantly the neonate starvation, and body, egg and neonate volumes in the offspring of B. calyciflorus. The neonate starvation times of all the thirteen clones were curvilinearly correlated with temperature. The neonate starvation times of Clone SP1 was negatively correlated with body volumes. The neonate starvation times of Clone SP2 was negatively correlated with neonate volumes, but that of Clone SU1, SU3 and SU7 were positively correlated with neonate volumes.

回归和偏相关分析表明，所有克隆的幼体耐饥饿时间均与温度间呈曲线相关；克隆SP1的幼体耐饥饿时间与母体体积间存在显著的负相关关系；克隆SP2幼体耐饥饿时间与幼体体积间有显著的负相关关系，克隆SU1、SU3和SU7的幼体耐饥饿时间与幼体体积间均有显著的正相关关系。

The values of EAAI in rotifer and nauplii of Artemia were all above 90 for different larval stages of Scylla serrata. It was suggested that rotifer and nauplii of Artemia may satisfy the nutritive requirement of larval Scylla serrata for amino acids in diet protein.

对于各期幼体，饵料轮虫和卤虫无节幼体的必需氨基酸指数均大于90，可以认为，轮虫和卤虫无节幼体能够满足锯缘青蟹幼体对饵料中必需氨基酸的营养需求。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失