幼

Group A(n=5) underwent gastroduodenal artery ligation and catheterization through the right gastroepiploic artery.

14只幼猪分为3组，A组和B组分别行胃十二指肠动脉及脾动脉结扎并置管；C组行左耳缘静脉穿刺。

With king cakes, beignets and other desserts from New Orleans native David Gaus.

西哈努克蛋糕，beignets和其他甜品，从新奥尔良原住民幼

Three donor wheat all show good character and had little genotypical difference in the aspect of inducement、differentiation、regeneration etc. They could all nice genotypical cultivars for wheat transformation. 3160 embryos have been used to transformation (960 embryos belongs to Atlas66, 1000 embryos belongs to EM12, 1200 embryos belongs to Scout66 respectively), At last, we got twenty-six of which were positive cultivars through PCR. The preliminary whole transformation frequency was 0.82 percent. Also the citrate synthase activities of the leaves of T_0 progeny show 5～9 higher than the control lines.

转化过程中分别使用了960个Atlas66、1000个EM12和1200个Scout 66幼胚(总记3160个)，经抗性筛选、CS与标记基因的PCR检测初步表明有26株为阳性植株，总转化率为0.82%；同时进一步对T_0代植株叶片柠檬酸合酶以及T_1代植株经铝胁迫后对其根尖分泌物进行检测，结果在上述转化植株中检测到了柠檬酸合酶的活性，且转化材料比对照材料平均高出了5～9倍；同时铝胁迫后，T_1植株根尖检测到了苹果酸及柠檬酸的分泌。

The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因，构建含ggpps基因的植物表达载体，用根癌农杆菌介导，将ggpps基因导入银杏幼胚并诱导出愈伤组织，PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况，高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量，筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

In size and shape, the newly metamorphosed juveniles were nearly identical to glochidia except for more globular.

刚刚变态好的幼蚌，在大小和形态上与钩介幼虫差异不大，只是略圆鼓些。

In the diet, the proportion of Platycephalidae, an important prey of juvenile A. sinensis in the past, was very low, only accounting for 1.81% of the total food by mass, while that of Gobiidae and Isopoda, which had not been recorded in the diet, was higher, accounting for 50.54% and 10.07% of the total, respectively.

与历史资料进行比较发现，曾是幼鱼重要饵料的鲬类所占比例极小，其质量百分比仅占1.81%；而以前未曾记录的虾虎鱼类在食物中所占比例较高，其质量百分比达50.54%；等足类以前也未曾记录，其出现频率达10.07%。

The bees, gorged with honey and no longer protecting their brood, are as docile as ladybugs.

此时，这些拼命地采蜜而疏于保护幼蜂的蜜蜂就像瓢虫一样驯服。

The research illustrated that,empty-shell chestnut occured chiefly on thephases of gynospore formationand young embryo development.

研究认为，板栗空苞主要发生在6月中旬大孢子形成时期和7月上旬幼胚发育时期。

The cause of hindering the gynospore formation was short of boronin chestnut orchard,which led to the poor pollination and a decrease ofendogenous GA1/3, iPAs level and an increase of endogenous ABA level in ovary,which restrained the mother cell division of gynospore.The low level ofendogenous GA1/3,iPAs contents and the increase of endogenous ABA contentcaused a serious lack of reducing sugar,starch,protein and potassium in ovary,which led to a cease of young embryo development.

其中，大孢子形成过程的停滞是由于栗园B素缺乏，致使授粉不良和子房内源GA1/3、iPAS等含量下降，ABA含量上升，从而抑制大孢子母细胞分裂；幼胚发育停滞则是由于子房内源GA1/3、iPAS等生长类激素的上升启动受阻，继续处于低水平，ABA上升，使子房中还原糖、淀粉、蛋白质和K素积累受阻，造成营养严重缺乏。

Keywords: Haemaphysalis longicornis; juvenile hormone; chemical structure; synthesis site; physiological function

中文关键词：长角血蜱；保幼激素；化学结构；合成位点；生理功能

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。