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Methods Since 1993,we had used uterus to cram presacral space in 27 female patients with rectal cancer after Hartmann′s operation and obsorved the pateints who were only stitched pelvic peritoneal and placed drainage tube in the presacral space.

自1993年以来,我们对27例女性患者采用子宫充填骶前腔,并与单纯缝合盆底腹膜、骶前并置引流管的传统方法比较。

That this fundamental book of religion, containing such a free and pure stream of truth, could be pseudepigraphic, and that the whole nation should have considered as of Mosaic origin and of divine authority, and have adopted at once, without objection or criticism, a book which was a forgery, of the existence of which no one knew anything before that time, and which demanded radical modifications of the religious life, and especially of worship, is inconceivable.

这本书的基本宗教,载有这样一个自由和纯流真理,可pseudepigraphic和整个国家应该考虑为花叶原籍国和神圣的权力,并已通过一次,无异议或批评,一个本书是伪造的,存在著没有人知道在此之前,任何时间,并要求彻底修改的宗教生活,特别是崇拜,是不可想像的。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育,MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用;RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1),以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况;Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达,以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用;免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达;激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位,并观察真菌刺激后人角膜上皮细胞骨架的变化;流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变;光学显微镜观察真菌与人角膜上皮细胞黏附数量,记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后,细胞超微结构的改变。

Objection: To investigate the efficacy of UV irradiation own blood and reinfusion into the patients with pulmonary heart disease.

目的:了解慢性肺心病患者血液经紫外线照射并加氧后回输前后血液流变学及血气变化情况,并观察其疗效。

Finally, simulation software was developed with Visual C++ and Delphi for 2D molding analysis of MIM, which affords facilities for the quality forecast of 2D thin wall parts and optimal design of mold and process parameters. In the research of molding performance of MIM feedstock, for the shortcoming of capillary rheometry, it was propounded that using spiral mold test molding performance of feedstock, and a set of spiral mold was creatively designed with quickly exchange gates and closed mold cavity. With the spiral mold, an orthogonal experiment was made on getting regress equation of flow length L〓 of the heavy alloy feedstock. Then the rule of L〓 varying with Q〓, P〓, and ΔT〓 was discovered. Q〓 ranks first in the influence on process parameters of molding performance, P〓 and Δ T〓 second. Therefore the injection rate Q〓 should be controlled primarily to improve the flow performance of feedstock.

在MIM喂料注射成形性能研究方面,针对毛细管流变仪测试法衡量流动性能的不足,提出使用螺旋线模具测试喂料熔体的注射成形性能,创造性地设计了一套可快换浇口,模腔封闭的螺旋测试模,并在螺旋测试模上采用正交实验设计,得出了高密度重合金喂料流动长度L〓的回归公式,揭示了L〓随Q〓、P〓、△T〓和S〓的变化规律,并得出工艺参数对成形性能影响的强度顺序为Q〓最大,P〓与△T〓其次,因而改善喂料流动性能应优先控制注射速率Q〓。

In the dry sliding experiments of composites, friction coefficients decreased with the increase of load. Plastic deformation happened in the worn surface where Fe element was found, so PAM micro-fiber supported part of the load and prevented the further deformation. On the condition of high speed and high load, the worn surface of PAM1/nylon 6 composites was nearly melted and formed comparative uniform transfer on the surface of steel, which accounted for the rather low wear mass loss. The infrared analysis showed that the a small quantity of transition of crystal form from α into γ of nylon and its composites happened during the friction because of the high temperature and strong sheering strength.

通过对复合材料耐磨性能研究表明:复合材料的摩擦系数随载荷的增加而逐步下降,且磨损表面有明显的塑性变形;从能谱分析结果来看,复合材料表面有Fe的存在,表明PAM微纤承受了部分载荷,并阻止了基体的进一步塑性变形及粘着转移;在高速高载时,PAM1/尼龙6磨损表面处于粘流态,并在钢环表面形成了连续的转移膜,表现出较好的耐磨损性能,且在高温和强剪切力的作用下,材料基体发生了少量的α晶体向γ晶体的转移。

Subcircuit models are designed and simulated, which includes Bias Current Source, Voltage Reference, Error Amplifier, PWM Comparator, Driver Circuit, Protection Circuits for Over-temperature, Over-current. At last, combined with periphery component, the circuit is simulated, and the result meets the anticipant requirement.

并对集成电路内的各个模块包括电流偏置电路、基准电压电路、误差放大电路、三角波振荡发生电路、PWM比较电路、驱动电路、过热保护电路和过流保护等进行了具体的设计和仿真,并对整体应用电路进行了仿真,结果均达到了预先设定的指标。

Any of numerous chiefly marine carnivorous fishes of the class Chondrichthyes, which are sometimes large and voracious and have a streamlined, torpedolike body, five to seven gill openings on each side of the head, a large oil-filled liver, a cartilaginous skeleton, and tough skin covered with small toothlike scales.

中文翻译:鲨鱼任一种软骨鱼纲的海生食肉鱼,有的体形巨大、贪婪并呈流线形,有鱼雷状躯体,头的侧面各有五至七个鳃孔,并生有一个大的富有油脂的肝脏,一个软骨骨架和覆盖有小齿状鳞片的坚韧表皮

Any of numerous chiefly marine carnivorous fishes of the class Chondrichthyes, which are sometimes large and voracious and have a streamlined, torpedolike body, five to seven gill openings on each side of the head, a large oil-filled liver, a cartilaginous skeleton, and tough skin covered with small toothlike scales.

鲨鱼:任一种软骨鱼纲的海生食肉鱼,有的体形巨大、贪婪并呈流线形,有鱼雷状躯体,头的侧面各有五至七个鳃孔,并生有一个大的富有油脂的肝脏,一个软骨骨架和覆盖有小齿状鳞片的坚韧表皮 A person regarded as ruthless, greedy, or dishonest。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养,通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆;以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达,了解不同治疗阶段VEGF及其受体的表达状况,并结合临床指标进行分析,明确VEGF及其受体在白血病发生过程中的作用;流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析,初步阐明难治性白血病抗凋亡形成的原因;蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达,初步了解难治性白血病形成的分子生物学机制。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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