并流

The main work and the originality points of this dissertation could be concluded as the following parts:(1) To analyze systematically the mathematical model of LES of air flow in great space, under the case of isothermal and non-isothermal, as well as the combined action of buoyancy and strain. Some basic problems are studied about the LES application into airflow simulation of great space, such as boundary conditions, spatial difference scheme and time advancing scheme, time step etc., which creates the basis for the LES application to indoor airflow simulation.(2) Both the instantaneous and time averaged flow field of great space with multiple jets under isothermal and non-isothermal cases are explored with large eddy simulation method, and also discussed the dynamic characteristics and the law of fluidflow in the great space.(3) Based on the results of LES of the great space and the Fanger thermal comfort indexes, it is brought forward the concept of the series of dynamic thermal comfort evaluating indexes, which could be divided into thermal comfort index with time averaged properties PD, PPD, PMV, thermal comfort index with instantaneous properties IPD, IPPD, IPMV, and time averaged thermal comfort indexes TAPD, TAPPD, TAPMV, and transient situations time averaged thermal comfort indexes TTAPD, TTAPPD, TTAPMV. The differences among them and the calculation methods are discussed, and the four kinds of indexes are calculated with the thermal comfort index PD as an example.(4) Based on the LES results it is discussed the hot air stratification phenomena in air-conditioned buildings in the case with air supply and return registers on the ceiling and the case on the sidewall. The fundamentals of the hot air stratification are studied and the relations of estimating hot air stratification are brought forward.(5) With the advanced apparatus such as hot wire film anemometer IFA300 and laser particle field anemoscope, corresponding model test and site measurements have been done, which are compared with the simulation results and LES is proved a very promising method in air flow simulation indoor.

本文主要工作既创新点体现在以下几个方面：(1)系统分析了等温、非等温和考虑剪切力与浮升力综合作用的高大空间大涡模拟数学模型，并研究了高大空间大涡模拟在室内气流仿真应用中的一些基本问题，如边界条件、空间离散格式和时间推进格式、时间步长选择等问题，为大涡模拟在室内气流计算中的广泛应用打下了基础；(2)首次用大涡模拟方法研究了高雷诺数下高大空间多射流在等温、非等温情况下的瞬时流场和时均流场分布，并探讨了多射流流场的动态特性和流动规律；(3)基于大涡模拟的动态仿真结果和Fanger的热舒适指标，首次系统地提出了动态热舒适评价指标体系的概念：即基于时间平均参数的热舒适指标PD、PPD、PMV；基于瞬时参数的瞬时热指标IPD、IPPD、IPMV；基于时间平均热舒适指标TAPD、TAPPD、TAPMV；以及沿行动迹线的时间平均热舒适指标TTAPD、TTAPPD、TTAPMV，并分析了四类热舒适指标的差异性和计算方法，还以PD值为例对四类指标分别进行了计算；(4)基于数十种工况下空调房间大涡模拟的结果，研究了空调建筑上送上回和侧送侧回两种情况下热分层现象，并探讨了热分层的基本规律，首次提出了避免热分层现象的判断公式；(5)在暖通空调领域，首次使用IFA300热线风速仪、激光粒子速度场仪等先进设备，完成了与大涡模拟相对应的模型试验，并把实测结果与仿真结果进行了对比，说明LES在室内气流仿真方面是一种很有前景的方法。

This paper studies the main geometrical parameter such as angle, height, width, stagger and the upper width in dentation and trapezoid flow path, firstly, uses the single-phase numerical simulation of the computational fluid dynamics technique to analyse the influence of different geometrical parameter on discharge coefficient and flow state exponent of drip emitter. Secondly, uses the two-phases numerical simulation of CFD (1% sand content ) to analyse the influence of the above mentioned geometrical parameter on the contribution regulation of sand and anti-clogging performance. Thirdly, uses the PIV （ particle Tracking Velocimetry ） technique which can see the small-scale flow path to observe and test the trajectories and velocities of solid particles in labyrinth flow path, and analyses the basic factors which can influence the movement characteristic of solid particles and the flow state in flow path.

本文以齿形流道、梯形流道为主要研究对象，以流道转角、流道高，流道宽、偏差量及梯形流道上底宽为主要结构参数，首先应用 CFD 单相流数值模拟技术对不同结构参数灌水器流道水力性能进行模拟，分析了不同结构参数对流态指数和流量系数的影响，其次，应用 CFD 两相流（含沙量为1%）数值模拟技术分析了结构参数对含沙量分布规律和抗堵塞性能的影响，然后，应用 PIV 可视化测试方法测试了固体颗粒在流道内运行轨迹及速度，分析了影响固体颗粒运动特性及流道内流体流态的基本因素，最后，对上述流道结构形式通过模具加工及滴灌带试制，进行了标准化测试（ GB/T 17187-1997 农业灌溉设备滴头技术规范和试验方法与 ISO 标准 ISO /TC23 /SC18 /WG5N4短周期堵塞测试程序），测试结果验证了 CFD 模拟结果，并提出了迷宫结构流道灌水器的抗堵塞设计方法。

In this system, we introduces cluster architecture to provide global and scalable streaming caching proxy service mechanism; We implement streaming media transfer protocol on caching proxy end; We implement layered caching mechanism (global disk caching and memory caching) using dynamic sharing running memory buffer algorithm to support more stable and real-time parallel streams; We implement client request aggregation by means of streaming session sharing to reduce disk I/O and serve more user; We propose single streaming image to support the unify of pre-fetching streaming and caching streaming, which guarantees the proxy streaming of transparency for client; We design and implement streaming pre-fetching mechanism based on packet feedback information to reduce the number of "rebuffering" on client end; We bring forward streaming caching admission control policy fit for streaming caching proxy server to guarantee the QoS of streaming service.

该系统引入集群架构以提供全局的、可扩展的缓存代理服务机制；实现了缓存代理端的流媒体传输协议；采用动态共享运行缓冲区算法实现了分层缓存机制(全局磁盘缓存和内存缓存)以支持更多稳定、实时的并发流；利用流会话共享机制实现了客户请求聚集，从而减少磁盘I/O同时能服务更多的客户；提出了单一流映像，以支持预取流和缓存流的统一，从而保证代理端服务流对客户的完全透明；设计并实现了基于包反馈信息的流数据预取机制，从而减少了客户端的"rebuffering"次数；提出了适合于缓存代理服务器的流缓存准入控制策略，保证了流服务的QoS。

What this paper finished mainly work as follows: Five-phase hybrid step motor driver system with fixed-chopping frequency and constant total current driving is fully described. Mathematical model of the system, included the current control circuit, power driving circuit and five-phase hybrid stepping motor is established. The steady modeling of the system is established and studied. Based on the steady modeling of the system, dynamic modeling of the system is studied and single step response characteristic is constructed with MATLAB/Simulink. The simulation results, which is certified by the theory analysis, coincide with the theory analysis.

本文主要完成的工作包括几个方面：对五相混合式步进电动机恒频斩波恒总流驱动系统进行了完整的描述；建立了包括电流控制电路、功率驱动电路以及五相混合式步进电动机在内的恒频斩波恒总流驱动系统的完整的数学模型；基于MATLAB/Simulink建立了恒频斩波恒总流驱动系统稳态仿真模型，并进行了仿真；在建立其稳态仿真模型的基础上，对动态模型进行研究，并借助于MATLAB/Simulink工具建立了恒频斩波恒总流驱动系统单步响应特性的仿真模型并对其单步响应特性进行了仿真研究，仿真结果与理论分析相符。

In this thesis the process of constructing the non-perturbative Hamiltonian theory is de-scribed and is applied to estimate the vacuum condensate. It contains the following contents:At the very beginning, by using the path integral method and eliminating the gluon freedom, aGCM action 〓 of current quarks including lower order current-current coupling was derivedfrom the QCD Lagrangian and the effective Hamiltonian operator that could hardly be doneby the normal methods was derived. After doing this, the broken vacuum is introduced whichincludes quark-antiquark condensate through the generalized Bogoliubov-Valatin transformation,the effective Hamiltonian of constituent quark was derived. The detailed formulas containingthe spatial current-current coupling term for the effective Hamiltonian and gap equations wasworked out by parameterizing the correlation kernel as a quadratic potential. And then, the gapequation was solved and the quark-antiquark condensate of vacuum was studied both in the casesof instantaneous interaction and retarded interaction. In the end, the effective Hamiltionian withtwo-body quark-quark interaction was derived with one-body approximation, and with the helpof the functional integral method the coupling non-linear dynamic equations for systems withnuclear matter was derived. Finally, these equations were solved by selfconsistent method andthe effect of nuclear matter on vacuum condensate was studied. The spatial current-current coupling term is too difficult to handle, hence the correlationkernel is assumed to be not important and usually omitted in the pure vacuum condensate, andthe instantaneous interaction generally is adopted. Retaining the spatial current-current termand partial retardation effect, the quark pairs condensate in pure vacuum was studied, and theeffect of quark mass was also studied. At present, little study is focused in the case with nuclearmatter and spatial current-current term also omitted. Under the approximation with partialspatial current-current term, the effect of nuclear matter on vacuum condensate was studied.

本论文描述了量子色动力学整体色对称模型哈密顿量方法的构建过程，得到了反映正反夸克对凝聚真空结构的关于组分夸克的有效哈密顿量算符，它隐含了胶子作用，并且准确至流-流耦合项；接着，通过参数化哈密顿量中的夸克作用关联核，导出平方禁闭势参数化选择的哈密顿量的具体公式和能隙方程；随后，应用公式，编程求解，考察了瞬时作用下和部分延迟作用下真空的正反夸克对凝聚，在计算中保留了空间流-流耦合作用；之后，导出瞬时势和延迟势下包含二体作用项的哈密顿量公式，并采用单体化近似，通过泛函变分方法得到核物质存在时耦合的非线性动力学方程；在保留部分空间双流耦合作用的近似下，求解核物质的动力学方程，考察核物质密度对真空凝聚的影响，以往考察真空凝聚，对关联核的选用，由于空间流-流耦合项不易处理，也认为作用不大，常忽略该项，并且常采用瞬时作用；本文保留空间双流项和部分延迟作用，考察了真空情形的夸克对凝聚，还考察了夸克质量对纯真空凝聚的影响，以往对核物质存在情形的真空凝聚考察很少，也都忽略空间流-流项，本文在考虑部分空间流-流项近似下，考察了核物质存在对真空凝聚的影响。

The discrete velocity ordinate method in the kinetic theory of gases is developed and applied to discretize the correspo

借助非定常时间分裂法和无波动无自由参数的NND耗散格式，建立直接求解微观分子速度分布函数的有限差分数值格式；研究并发展可用于离散速度坐标点选取和离散速度空间宏观取矩的高斯─埃尔米特无穷积分方法、等均间隔的牛顿─柯斯复合积分法、以勒让德多项式的根为积分结点的高斯─勒让德数值积分法，并应用于不同马赫数绕流模拟；通过对不同流域一维激波管问题、二维圆柱绕流问题和三维球体绕流的计算研究，并将计算结果与其他途径得到的研究结果诸如DSMC模拟值、N-S解及有关实验数据进行比较分析，创建了一套能有效模拟稀薄流到连续流不同流域气体流动问题简化的统一数值算法研究框架

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Based on an analysis of distribution pattern of hydrocyclone"s axial velocity, by changing the inlet flow rate Qi and split ratio Fand making an investigation in the effect of Qi and F on axial velocity field, the factors causing the forming of short-cut circuit and circulating flow are found. It is considered that the short-cut circuit and circulating flow are produced by the structure of the hydrocyclone, and can"t be eliminated completely, though they interfere the flow field inside a hydrocyclone and affect the separation accuracy. Test results show that, by increasing Qi and F and controlling them in a certain range, the separation efficiency of the hydrocyclone can be improved.

在研究旋流器轴向速度分布规律的基础上，分析了短路流与循环流的形成原因，试验研究了改变操作参数，即改变入口流量Qi与分流比F对轴向速度场的影响认为短路流与循环流是由旋流器结构决定的，很难完全消除，它们干扰了旋流腔和大锥段的流场，在一定程度上也影响了分离精度试验结果表明，加大入口流量Qi与分流比F，并将其控制在一定范围内，便可提高分离效率这项研究对水力旋流器分离机理的认识与实际应用均有重要意义

By analyzing the general features of aquifer, a new concept of "the flow transfixion" was introduced, and the interaction between the flow transfixion and the heat transfixion and great significance of the flow transfixion to projects were analyzed.

通过分析含水层储能的一般特点，提出流贯通，分析流贯通和热贯通的相互作用、相互影响及对工程实际的意义，并基于地下水水热运移的基本原理，建立地下水流动数值模型，在此基础上对沈阳某场地水源热泵工程的运行进行流场及温度场的数值模拟分析，给出产生流贯通的依据，进而研究流贯通对热贯通的影响，并为该地区的地下水源热泵工程设计提供一定的参考。

Initially, assuming that the contact between cam and tappet is"dry contart", a dynamics model of Hertzin contact stiffness and impact damping is established. Next, with dynamic load on cam—tappet pair from the model, an analysis is made both to unsteady EHD oil film in cam—tappet pair under condition of full and partial lubrtication and to wear characteristics of cam profile. then, according to the fact that the lubrication in cam—tappet contact surface is EHD lubrication, taking the interaction between stiffness and damping of unsteady EHD oil film and rigidity of solid object contact into account, a dynamics equation of valve train relating to the dynamic effect of EHD oil film is built up and solved. Consequently, a further study is made on the dynamic characteristics of valve train under interaction of EHD oil film dynamic behavior and the problem of EHD lubrication in cam—tappet pair. Finally, the dynamic effect of full and partial EHD oil film is studied accordingly.

首先，通过对凸轮—挺杆接触为&干接触&的假设，建立了考虑赫兹&接触刚度&和冲击&阻尼&的配气机构动力学模型，用由此求得的凸轮—挺杆表面动载荷对凸轮—挺杆进行了全膜状态和部分膜状态非稳态弹流润滑分析，并研究了凸轮轮廓的磨损规律；然后，是在凸轮挺杆表面存在弹流动压油膜这一事实的基础上，相当于在动力学方程中考虑非稳态弹流油膜的&刚度&、&阻尼&和固体接触&刚度&的耦合作用，建立并求解了考虑弹流润滑行为动力学效应的配气机构的动力学方程，从而深入地研究了弹流润滑行为与动力学行为耦合作用下的配气机构动力学特征以及凸轮挺杆弹流润滑问题；作为前面工作的对应部分，考虑弹流油膜动力学效应的研究工作也同样分为全膜状态和部分膜状态进行。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。