并殖吸虫病

Sera from patients with other parasitic diseases and healthy individuals were used as control. RESULTS: The anti Trichinella antibodies were detected in 15 infected mice as early as 2 weeks after infection,and in 86 5% of 467 patients with trichinosis.

结果：15只小鼠感染旋毛虫后2wk即可检出抗体，467例旋毛虫病患者的抗体阳性率为86.5％，而丝虫病、包虫病患者各45例和健康人血清56份均为阴性。48份并殖吸虫病患者的交叉反应率为16.67％。

Circulating antigen was detected in 29 out of 70 cases with paragonimiasis with a sensitivity of 41.5%. The rate of cross reaction in cases with clonorchiasis sinensis and schistosomiasis was 25%（5/20） and 20%（4/20）, respectively, and it was negative in 60 casess with other parasitic infections and healthy subjects, with an overall specificity of 93.6%.

用CAg-dot-ELISA检测70份卫氏并殖吸虫病临床诊断患者血清，阳性29份，敏感性为41.5%（29/70），与华支睾吸虫病和日本血吸虫病患者血清分别有25%（5/20）和20%（4/20）的交叉反应，与其他寄生虫感染者血清和健康人血清（60份）均为阴性，特异性为93.6%。

Methods ELISA was used to detect the specific IgG and IgG 4 antibodies in sera from the patients with clonorchiasis and other parasitic diseases and from healthy persons. Results The sensitivity and specificity of IgG 4 antibodies detected by ELISA in sera from the patients with clonorchiasis showed no significant difference with that of IgG detected.

用华支睾吸虫成虫可溶性抗原进行ELISA分别检测华支睾吸虫病患者(76例)血清中的特异性IgG和IgG4 抗体及日本血吸虫病、并殖吸虫病、囊尾蚴病患者(分别为 63、3 5、41例)和健康者(65人)血清中的交叉抗体。

Objective: To study the disposition of epidemic focus and species of Paragonimiasis and infection condition of people, and the independence of P.

目的：研究福建省三元区并殖吸虫病疫源地的虫种分布和人群感染情况，并通过形态观察和分子生物学鉴定研究泡囊狸殖吸虫的独立性。

METHODS: Using microwave irradiation ELISAand fast ELISA to detect specific antibodies in sera from 118 cases with schistosomiasis japonica, 61 healthy individuals and 12 paragonimiasis cases.

用微波辐射ELISA和快速ELISA同步检测各期日本血吸虫病患者血清118份，健康人血清61份，并殖吸虫病犬血清12份。

Objective To identifiy the etiologic agent from a paragonimiasis patient using molecular techniques.

运用分子生物学技术分析虫卵基因序列鉴定并殖吸虫病类型。

Results Sera from 150 patients with schistosomiasis were detected, the average sensitivity was 84.7%(72.0%～91.0%). No false positive reaction was detected in 40 normal controls and none but 2 patients(2/10) with paragonimiasis showed cross reactions among 74 cases with other parasitic infections.

结果 用于检测 15 0例血吸虫病患者的循环抗原，其敏感性平均为 84.7%(72 。0 %～ 91.0 %)，40例正常人未出现假阳性反应，74例其他寄生虫感染者中，有 2例并殖吸虫病患者阳性，其余均未出现交叉反应。

Results Serum samples were tested by ELISA using SEA treated with sodium periodate (SP-SEA- ELISA), including 64 sera from cases with chronic schistosomiasis japonica, 119 sera from normal individuals in non-en- demic area, 34 sera from patients with clonorchiasis, 33 sera of paragonimiasis cases and 36 sera from patients with cysticer- cosis.

结果分别用两种方法检测患者血清，其中慢性血吸虫病64例、华支睾吸虫病34例、卫氏并殖吸虫病33例、囊尾蚴病36例，检测健康人血清119例。

Methosd The complete nuclear ribosomal DNA second internal transcribed spacer (ITS2) gene sequence of eggs in sputum from a paragonimiasis patient was obtained by directly auto-sequencing its PCR product .

先从并殖吸虫病患者痰中分离出虫卵，然后PCR扩增出虫卵中完整的核糖体DNA第二间隔区基因(ITS2 )，并直接用于测序从而获得该基因的核苷酸序列。

Among these patients, 20.9%(24/115) had a history of eating raw meat. For discharge diagnosis, neurocysticercosis accounted for 92.2%(106/115),cerebral paragonimiasis 3.5%(4/115), sparganosis 2.6%(3/115), and angiostrongyliasis cantonensis and gnathostomiasis 0.9%(1/115) each.

有生食史的占 2 0 。9%(2 4 / 1 1 5)，出院诊断为脑囊尾蚴病的占 92 。2 %(1 0 6/ 1 1 5)，脑并殖吸虫病占 3 。5 %(4/ 1 1 5)，脑裂头蚴病占 2 。6 %(3/ 1 1 5)，脑广州管圆线虫病、棘颚口线虫病各占 0 。9%(1 / 1 1 5)。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。