平衡值

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

If the Translucency Weight is 1, all of the transparency is used for translucency and there is no real transparency anymore

定义透明和半透明之间的平衡，如果该值是 1 ，所有的透明将用于半透明效果，实际上没有透明效果。

Main results are listed as below: 1. In rapid solidification, when the cooling rate is over some critical value, the solidification system will become unstable in the sense of the nonequilibrium thermodynamics, a uncontinuous steady state jump phenomenon will occur, and this uncontinuous steady state jump can be understood as the glass transition.

主要得到了如下结果：(1)通过对快速凝固过程的动力学及热力学的分析，提出了非晶转变的分枝理论模型，结果表明，对一合金熔体，当冷却速度大于某一临界值时，凝固体系会出现非平衡热力学意义上的失稳而出现多定态分枝现象，这一多定态现象可以被理解为非晶转变。

It was used to simulate the xenon poison in ship nuclear reactor with the burnup of 30 MW·d,and the simulated results were compared with that from point model.

结果表明：点模型的仿真结果偏小，用本软件进行仿真，平衡氙毒计算值与实测值间的偏差为-0。

On the safe side, we should use active soil pressure and passive soil pressure to calculate the support strength before T increased. That is RA=EA-EP Among them: RA ………… holding strength EA ………… composite force of active soil pressure EP ………… composite force of passive soil pressure Immediately, flexural torque distribution of enclosure wall can be counted. Mmax is the location of shear force zero. Equivalence beam method abridges. No matter which kind of supporting structure form, the key problem lies in the establishment depth of catch point, severity of support and enclosure wall, penetration depth and pit bottom quality of soil property.

单道锚杆支护结构计算分别采用静力平衡法及等值梁法，根据试算法插入深度t值需先进行假定墙的插入深度确定后来计算支撑力，为了安全起见，可按插入深度增大前的主动与被动土压力合力计算支撑力即 RA=EA-EP 式中：RA …………支撑力 EA …………主动土压力合力 EP …………被动土压力合力随之可求围护墙的弯矩分布，Mmax为剪力为零点的位置，等值梁法的计算略述。

Under different combustion pressuring rates of the solid motor, the relations among the convecting combustion flow characteristics, especial the pattern of flame spreading, and the variation of stress intensity factor at the crack tip are discussed in detail.

文中详细描述了在固体火箭发动机燃烧室环境中，药柱裂纹对流燃烧流场特性和固相裂纹体变形特性；燃烧室增压率对裂纹中火焰传播方式的影响；燃烧室增压率、燃烧室平衡压力的设计值、药柱裂纹径向长度对固相裂纹尖端应力强度因子、燃烧室压力的影响。

It is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.

该培养基含碳酸氢盐缓冲体系，在5%二氧化碳/95%空气培养箱中平衡时PH值为7.4。

It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.

该培养基含HEPES和碳酸氢盐缓冲体系，在5%二氧化碳/95%空气培养箱中平衡时PH值为7.4。

After buffer exchange the protein was concentrated to 300 ml and run on a column of Superdex 200 resin (14 by 100 cm) which had been equilibrated with 10 mM HEPES (pH 8.0), 150 mM NaCl, and 1 mM TCEP.

后缓冲交流的蛋白质集中在300毫升和运行的一列superdex 200树脂（ 14 100厘米）已平衡与10毫米hepes （ pH值8.0 ）， 150毫米，氯化钠，和1毫米的tcep 。

It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.

该培养基缓冲体系为重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。