平板细菌计数

Before and after lysin treatment, the cell morphology was studied by scanning electron microscopy. The results showed that the recombinant PlyC could specifically cleavage Streptococcus pyogenes (group A β-hemolytic streptococci).

用透析复性方法得到目的产物重组链球菌噬菌体裂解酶PlyC，以浊度法和平板计数法检测其体外抗菌效果，扫描电子显微镜观察裂解酶作用前后细菌细胞形态变化。

METHODS: The concentration of ozone that was transmitted by ozone generator at different time was determined by using iodine titrimetric method. According to the bactericidal assay of quantitative vehicle, the bacteria on the ozonized vehicles and unozonized vehicles was washed, 50μl eluant was seeded on the TSA plates. The TSA plates were put into the anaerobiotic incubator (90% N2, 10% CO2, 37℃). After 24 to 48 hours, the CFU on the plate was counted.

用碘滴定法测定打开臭氧发生器不同机组及不同时间时的臭氧浓度，按载体定量杀菌试验，将经臭氧作用的载体与相同环境下未经臭氧作用的载体上的细菌洗下，各取洗脱液50μl，接种于TSA平板并置厌氧箱(90%N2， 10%CO2， 37℃)培养24~48h后，进行活菌菌落计数。

According to the bactericidal assay of quantitative vehicle, the bacteria on the ozonized vehicles and unozonized vehicles was washed, 50μl eluant was seeded on the TSA plates.

用碘滴定法测定打开臭氧发生器不同机组及不同时间时的臭氧浓度，按载体定量杀菌试验，将经臭氧作用的载体与相同环境下未经臭氧作用的载体上的细菌洗下，各取洗脱液50μl，接种于TSA平板并置厌氧箱（90％N2，10％CO2，37℃）培养24-48h后，进行活菌菌落计数。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。