平板细菌计数

RESULTS: We built up two standard curves with the results of UV spectrophotometry and wet weight, to plate count, respectively. The analysis of accuracy rating proved that there was an approximal linear correlation between the two standard curves ranging from 105/mL to 108/mL.

结果： 建立了紫外分光光度计法和湿重法相对于平板计数法的标准曲线，通过准确度分析表明在细菌含量在每毫升105～108之间时，两标准曲线线性关系良好。

With the denaturation and protein refolding, we gained the recombinant PlyC. To determine its biological activity, we adopted turbidimetry and plate count method. Before and after lysin treatment, the cell morphology was studied by scanning electron microscopy.

用透析复性方法得到目的产物重组链球菌噬菌体裂解酶PlyC，以浊度法和平板计数法检测其体外抗菌效果，扫描电子显微镜观察裂解酶作用前后细菌细胞形态变化。

Samples of bacterium taken from Huguangyan Maar Lake are isolated and purified using spread plate method and streak plate technique. The partial characteristics is analyzed morphologically and physiologically.

采用涂布法、划线分离法以及形态学和生理生化特性分析，对湛江湖光岩微生物进行了分离纯化及部分性质研究；同时用平板菌落计数法对其异养细菌、真菌和弧菌等的数量进行初步统计。

METHODS: We counted M. vaccae by UV spectrophotometry, wet weight measurement and plate count method, and built up two standard curves. One was drawn according to the results of UV spectrophotometry to plate count and the other was humid weight to plate count.

采用紫外分光光度计法、测湿重法、平板菌落计数法测定细菌数，建立紫外分光光度计法、测湿重法对平板菌落计数法的标准曲线。

Vibrio parahaemolyticus 1211U was cultured in sterilized sea water by the process of filtration at 4 ℃;50-60 days later, plate count and most probable number showed that there were no culturable bacteria.

将副溶血弧菌菌株1211U培养于低温贫养条件下，以平板菌落计数法和最大近似数法检测可培养细菌数，50～60d后表明可培养数下降为零。

Study of kinetic antibacterial activities of QJ2 against pathogen W-1 was tested by viable bacteria counts and absorbency at 600nm: 106～ 107 cfu/mL cells of W-1 began to decrease in 15 min after mixing with CFS(cell free-supernatant) of QJ2; the bacterial counts decreased to a minimum at 4h, and the numbers of bacteria began to increase after 6h. The cells of QJ2 did not show any sensitivity to its inhibitory substances in CFS. The size of active molecule in the CFS of QJ2 was smaller than 8000 Da by measuring the inhibitory activities of the fluid inside and outside the dialysis cellulose tubing. QJ2 was identified as Flavobacterium ordaratum by traditional morphological and biochemical method and API-20 identification system.

并对拮抗菌QJ2的拮抗作用进行了研究，结果为：QJ2有广泛的弧菌抗菌谱，对37株弧菌的抗菌阳性率达到89.2%(33/37)；用活菌平板计数法和O.D.600nm研究了QJ2培养物的去细胞上清液与病原菌W-1的作用动力学，15min时W-1的细菌数便开始减少，4h时细菌数最少，6h后开始增加，而对照组的细菌数呈逐渐上升趋势；QJ2对自身的拮抗物质不敏感；QJ2抗性物质的分子量不大于8000Da；经常规生理生化方法和API-20细菌快速鉴定系统鉴定，QJ2为气味黄杆菌。

By the methods of direct viable count and plate count, this paper studied the effects of different selective extractants on the bacteria, algae and protozoan on the biomembrane in natural water body.

用平板计数法、显微镜直接计数法及培养法分别研究了选择性萃取剂对自然水体生物膜上细菌、原生动物和藻类的影响。

With several different brands of soymilk collected in the market,we have test the microorganism content in the soymilk by using the methods of plate count,methylene blue reductase testing and direct microscope count.

以市售不同品牌的饮用豆奶为试验材料，采用平板菌落计数法、美蓝还原酶试验法和显微镜直接计数法对豆奶中的细菌含量进行检测。

Since the dilutions plated are replicates of each other, determine the average of the duplicate bacterial counts per ml of sample and record in the chart.

因为稀释平板培养都是彼此的重复者，测定每毫升样品的重复细菌计数的平均数，而且登录在纪录纸上。

Root soils of Vitis vinifera cv. Cabernet sauvignon grapes of 4 and 8 years old in Yangling region vineyards were taken as samples; and the methods of plate culture and selective medium were used to culture and separate soil microbes. Then the microbes separated from different regions were counted and classified.

以陕西杨陵4年生和8年生赤霞珠葡萄的根系土壤为试验材料，利用平板培养法和微生物选择培养基进行培养分离，采用梯度平板涂布法计数，对葡萄根系土壤中的7种目标微生物（细菌、真菌、放线菌以及4种细菌生理群）在不同区域、不同层面的分布和数量进行比较、分析。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。