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ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.

针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状,即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况,本研究从筛选玉米转基因工程所需要的优良受体入手,采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法,以及现代分子标记定位主效QTL分析方法,对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究,取得以下主要研究结果:(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合,在相同培养条件下,对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定,发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数,但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系,有的基因型,如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异;有的基因型,如自交系综31,仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599,但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距,仅为19-599和18-599的1/3左右;有的基因型,如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低,平均仅在3%左右,而且胚性愈伤组织绿苗发生数表现也很差,基本上没有分化成苗。

In order to explain clearly the variation status of embryo from tetrapoid Robinia pseudoacacia and fully use the variation resource initiated in the sexual reproduction of TRP in the further improvement of TRP, the classification and vitalities of seed embryos from tetraploid Robinia pseudoacacia were studied. The main results are as follow. According to the cotyledon number, color and plumpness, the seed embryos of TRP could be divided into 5 types: YVT (yellow, very replete,2 cotyledons), YMT( yellow, more replete,3 cotyledons ), YRF ( yellow, replete,4 cotyledons ), GNT ( green, no-replete, 2 cotyledons ) and WNT ( white, no-replete, 2 cotyledons ).

为了阐明刺槐同源四倍体种子胚变异状况及充分利用刺槐同源四倍体有性过程创造的变异资源对四倍体刺槐进行遗传改良,研究了刺槐同源四倍体种子胚类型及其生活力特征,主要结论如下:刺槐同源四倍体种子胚按照子叶数量、颜色及饱满程度,可以划分出5个类型:黄二、黄三、黄四、绿二及白二,所有类型种子胚混合平均单胚重量接近二倍体的1/2。

During the first two weeks after birth, the growth performances were not significant between the mutant mice and the normal ones, but after that, the mutant mice grew much slower. The differences were significant in the indexes of eyes, lungs, thymuses, and adrenal glands between the genders. At the same time, the differences were significant in the organ indexes, such as lungs, adrenal glands, uterine and testicles between the normal phenotype mutant mice and the abnormal ones. And these two kinds of mice showed different amount of hemoglobin and red blood cell.

结果:B6-Co小鼠不育率22%、平均产仔数5.62±2.55只、离乳成活率56.59±41.22显著低于正常的B6小鼠,而胎间隔29.50±10.50天比正常B6小鼠短。B6-Co小鼠出生后2周内生长发育与正常B6小鼠基本一致,2周后,其生长发育慢于正常B6小鼠。B6-Co小鼠雌雄间的眼球、肺、胸腺、肾上腺的脏器指数差异有统计学意义。B6-Co表型异常小鼠与B6-Co表型正常小鼠相比,肺、肾上腺、子宫、睾丸的脏器系数差异有统计学意义。B6-Co小鼠表型异常小鼠在血红蛋白和红细胞数量上异于B6-Co表型正常小鼠。

Results: B6-Co mice showed higher sterile rate, and significant lower average litter size and survival rate at weaning than the normal B6 mice, but the time interval between deliveries was shorter in the mutant population. During the first two weeks after birth, the growth performances were not significant between the mutant mice and the normal ones, but after that, the mutant mice grew much slower. The differences were significant in the indexes of eyes, lungs, thymuses, and adrenal glands between the genders. At the same time, the differences were significant in the organ indexes, such as lungs, adrenal glands, uterine and testicles between the normal phenotype mutant mice and the abnormal ones. And these two kinds of mice showed different amount of hemoglobin and red blood cell.

结果:B6-Co小鼠不育率22%、平均产仔数5.62±2.55只、离乳成活率56.59±41.22显著低于正常的B6小鼠,而胎间隔29.50±10.50天比正常B6小鼠短。B6-Co小鼠出生后2周内生长发育与正常B6小鼠基本一致,2周后,其生长发育慢于正常B6小鼠。B6-Co小鼠雌雄间的眼球、肺、胸腺、肾上腺的脏器指数差异有统计学意义。B6-Co表型异常小鼠与B6-Co表型正常小鼠相比,肺、肾上腺、子宫、睾丸的脏器系数差异有统计学意义。B6-Co小鼠表型异常小鼠在血红蛋白和红细胞数量上异于B6-Co表型正常小鼠。

A total of 164 ships of these dimensions transited the canal last month, breaking the previous record of 159

此外,平均每天该尺寸船舶登记数量达到5.29条,超过2006年11月创下的每天5.17条记录。

Results Following orchiectomy 7d,the amount of CGRP-immunoreactive nerve fibers in the AP of the rat increased significantly, with the tortuous varicose fibers more densely distributed than that of NOR and the amount of varicosities increased significantly, while the thick fibers were more thicker than that of NOR,and many thin fibers were sent out from the thick fibers, and the varicosities were more larger.

结果 睾丸摘除7d后,大鼠垂体前叶CGRP免疫反应神经纤维发生了显著变化,表现为膨体型纤维较正常组稠密,分支增多,迂曲成片,盘绕成网,分布更加广泛,膨体数量显著增多;粗纤维较正常组粗,且发出许多迂曲盘绕的细纤维,膨体形态变大。t检验显示,OX组与NOR组垂体前叶内CGRP免疫反应神经纤维的平均面积密度差异显著,OX+V与NOR组差异显著。

In order to discuss the harm of long-time surfing the internet to the human body, we extract 35 undergraduate in our university and carry on the net continuously experiment. At the same time, 23 people (male 15 person, female 8 person) have been sampled on the track inspection on routine blood index. We discovered that continual 56 hour surfers can c ause obvious blood corpuscle metabolism function disorder, There are radical ascensions concerning the absolute value of blood routine inter cells, the number of white blood cells, the volume and quantity of the blood platelet and so on. The average volume of the red blood cells reduces significantly. The distributing volume and width of the red blood cells increase correspondingly.

为了探讨长时间上网对人体的危害,我们抽取在校本科大学生35人进行连续上网实验,对其中的23人(男15人、女8人)进行了跟踪血常规检查,结果发现连续56小时的上网,导致受试者出现明显的血细胞代谢功能紊乱,血常规中间细胞绝对值、白细胞计数、血小板体积及数量等多项指标出现应激性升高,平均红细胞体积显著性降低,红细胞体积分布宽度百分比明显增高。

Fruit weight was significantly increased by 33 g or 79 g through half-tree of three out of four flower cluster thinning,consequently fruit calcium concentration was decreased by 1.62 or 2.66 mg/100g fresh weight respectively,it means that fruit calcium concentration was significantly affected by the treatments that significantly altered fruit size.

苹果树疏除花序1/2或3/4的数量使苹果平均单果重比不疏花分别显著增加了33 g或79 g,而果实钙浓度每百克鲜重分别降低了1.62 mg或2.66 mg,充分说明当疏花显著地影响果实大小时,果实内钙浓度也会明显地发生相应的变化。

Census of Crow Pheasant was conducted in Guangdong Haifeng Avian Nature Reserve in 2005. Among four seasons, there were thirty two individuals recorded in summer which was the most. The mean-individual of each line in summer was significantly higher than that in spring and autumn (P.05). The population density in summer was the highest, 17.78 ind./km^2 vs. 1.11 ind./km^2 in winter which was the lowest.

为掌握褐翅鸦鹃在广东海丰鸟类自然保护区的种群现状,提出有针对性的保护措施,2005年对保护区内褐翅鸦鹃数量行了调查。4个季节中,夏季记录的个体数最多(32只),其样线平均个体数与春季、秋季相比,差异性显著(P.05);夏季种群密度最大,达17.78只/平方公里,冬季仅为1.11只/平方公里。

Result shown: value of fractal dimension is a comprehensive index, which reflect the changes of landscape, patch areas, patch numbers, area per patch, and their interaction. And the index can reflect the irregular degree and the complex degree of landscape patch in dumping place. The fractal dimension of mixed vegetation of arbors – shrubs and mixed vegetation of shrubs maintain the high level, while fractal dimension of new reclamation land and grass land are low. The difference of other type landscape fractal dimension is not significant. Vegetation landscape is influenced by many kinds of factors. It is primarily influenced by surface material composition and terrain in south and west dumping place, which diversity index is high. It is primarily influenced by vegetation type in inner dumping place and expanded west dumping place. The landscape multiplicity index of the mixed vegetation of grass and shrubs is higher than other landscape type, but landscape dominance index of this area maintains on the lower level.

结果表明:分维数的大小反映了景观斑块面积、斑块数量和平均斑块面积等因素的相互作用,反映了排土场内景观斑块的不规则程度和复杂程度;乔灌混交地和灌木混交地保持着较高的分维数,新造复垦地和草地的分维数较低,其它景观类型的分维数则差别不大;植被景观受多种因子的影响,在南排土场、西排土场受地表物质组成和地形的影响为主,其多样性指数较高,在内排土场、西排扩大区受植被类型的影响为主;草灌混交地的景观多样性远高于其它现有的复垦植被区,但其景观优势度则保持在较低水平上。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?