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Results: Among the patients who had normal joint laxity, the mean side-to-side difference in anterior tibial translation on testing with the KT-2000 arthrometer at a minimum of twenty-four months postoperatively was 2.7 ± 1.9 mm in the bone-patellar tendon-bone graft group and 3.5 ± 1.7 mm in the hamstring graft group. This difference was not significant.

结果:关节松弛度正常病人术后24月利用KT-2000 型关节测量仪检查平均双侧胫骨前方移动度差别,髌骨-骨-腱移植组为2.7 ± 1.9 mm ,筋膜移植组为3.5 ± 1.7 mm ,差别无统计学意义。

3To introduce a minimally-invasive technique of a new percutanous external spinal fixation assistanted minimally-invasive surgical procedure treating thoralumbar fracture and analyse its clinic applicable area and value. [1]According to advantage of spine external transpedicular fixation technique and limitation of traditional percutanous external transpedicular fixation,design a new percutanous external transpedicular fixation.

(1)根据脊柱外固定技术的优点,传统脊柱外固定器的不足自行研制一套新型脊柱外固定器(WS-1型);(2)取7具新鲜小牛腰椎标本,自制实验不稳定性骨折模型,把实验分成正常A组、骨折模型B组、新型脊柱外固定器固定C组、新型脊柱外固定器固定联合PVP技术D组,分别按顺序测试四组的三维稳定性,并测试A组、C组与D组在300N轴向压缩实验,再行统计学分析比较;(3)应用新型脊柱外固定器联合椎间盘镜微创椎管减压技术和PVP技术手术治疗胸腰椎骨折,病例选择:本组病例共69例,年龄自10岁-70岁,平均年龄56.3岁。

Slit-lamp biomicroscopy photography, and immunohistochemical staining were done. The area of CNV was calculated and the average optic density of immunohistochemical staining of iNOS was measured after 8 hours and 1, 4, 7, 14 and 28 days by imaging analysis system.

采用裂隙灯照相,免疫组化染色,多媒体彩色图像病理分析系统分别计算8 h、1,4,7,14,28 d共6个不同时间点的角膜新生血管面积和诱导型一氧化氮合成酶免组染色的平均光密度值。

The factors which affect the average molecular weight of the polymer of AM and DMDAAC were investigated. The optimal reaction conditions are as follows: the concentration of monomer AM was 30%, while DMDAAC was 5%; the reaction temperature was 30℃, the amount of initiators at the low temperature stage was 0.05‰(compared to the weight of the monomers joining the polymerization), while V-50 was 0.025‰, the weight ratio of potassium persulphate to sodium bisulphite is 1:1, the amount of carbamide was 40‰, and complexing agent was 0.05‰, besides, aerate nitrogen gas for 8min, then the high molecular weight polymer (M=1.217×10^7) could be got after 16h reaction.

研究了影响AM与DMDAAC共聚物的平均分子量的因素,得到了优化条件为:单体浓度为30%,DMDAAC与AM摩尔比为5:95,聚合温度为30℃,低温段引发剂用量为0.05‰,K2S2O8与NaHSO3质量比为1:1,偶氮二异丁基脒盐酸盐(V-50)用量为0.025‰,尿素用量为40‰,乙二胺四乙酸二钠(EDTA-2Na)用量为0.05‰,在此优化条件下聚合反应16h后得到分子量为1.217×10^7的阳离子型聚丙烯酰胺。

Methods From March 2002 to January 2006, 41 patients with Tossy type Ⅲ aeramiaclavicular dislocation were treated with AO/ASIF clavicular honk plates. The coracoclavicular ligaments were sutured in 15 eases, and the plates were removed 12 months postoperatively in 18 patients.

自2002年3月~2006年1月使用AO/ASIF锁骨钩钢板治疗41例Tossy Ⅲ型肩锁关节脱位患者,其中15例术中修补了喙锁韧带,18例在平均术后1年取出钢板。

The results suggested that compared with A-starch and maize starch, there are more quantities of impurities, such as protein, lipid and pentosan, in B-starch. B-starch prepared in the laboratory almost has the same compositions as three samplings, and accords with the reports of some documents too. Differing from the granules in A-starch, those in B-starch are smaller sized (1-10m)and oval shaped, and felted each other. The crystallogram of A- and B-starch are similar, i.e. A type. There is difference in granular distribution between A- and B-starch. The granular distribution of B-starch is anomalous and extensive (l~1259m).B-starch has bigger dilatancy and retrogradation, and smaller blue value, solubility, viscosity, gelatinization temperature and gelatinization enthalpy.

试验结果表明:跟玉米淀粉、A淀粉相比较,B淀粉含有的蛋白质、脂肪和戊聚糖等杂质数量多;实验室制备的小麦B淀粉中淀粉、粗蛋白、戊聚糖等含量均与文献的报道接近,也与在工厂采样的B淀粉相近;与A淀粉不同,B淀粉以小颗粒的淀粉为主,平均粒径1~10μm,颗粒形状呈椭圆形,同时颗粒和颗粒之间黏结紧密;B淀粉和A淀粉晶形结构相似,都为A型;B淀粉的粒度分布情况不同于A淀粉,其粒度分布规律性不强,分布范围也较宽,粒度在1~1259μm之间;B淀粉的凝沉值和膨润力大于A淀粉,而沉降体积、蓝值、溶解度、粘度、糊透光度、相变温度和热焓均小于A淀粉;杂质的含量对B淀粉性质的影响是明显的,随着杂质含量的增加,沉降体积、溶解度和透光度减小,膨润力增大,但是它们对凝沉值、蓝值和粘度的影响比较复杂。

The results are as follows:The relationship between the precipitation and the stemflow is a linear regression. The stemflow of six forest types is Broadleaf forest, Conifer and broadleaf forest, Cupressus funebris forest, Mixed conifer forest, Pinus massoniana forest and Shrubbery and grass land in order.

结果如下:树干茎流量与降雨量成线性相关,回归方程达到极显著水平;6种森林类型的树干茎流量从大到小依次为阔叶林、针阔混交林、柏木林、针叶混交林、马尾松林和灌草地;不同树种间的树干茎流量差异极显著,影响树干茎流量的主要树体特征因子是冠型结构、胸径和平均冠幅。

Seven main effect QTLs and 7 digenic interactions controlling heat tolerance during flowering time were detected in two-year experiments.

利用181个在亲本间具有多态性的SSR标记,构建了覆盖水稻全基因组1559.6cM、标记间平均距离为8.3cM的遗传连锁图谱。2年共检测到7个抽穗开花期耐热性主效应QTL以及7对上位性QTL.2004和2005年分别检测到3个和4个QTL,共解释29%和37.3%的表型变异。

The data byχ~2 were consistent with the separation ratio as 1: 2: 1 χ~2=4.028<χ_(0.05,2~2=5.99, which further conformed that glandless character was controlled by a incomplete dominance gene, Gl_2~e. 2. SSR markers every about 5cM were selected in the chromosome 12 of our lab genetic linkage map to screen the parent plants. The markers with the polymorphism were used to amplify the DNA of 210 F_2 segregating plants selected randomly and recorded the marker genotypes. After eliminating the segregation distortion markers byχ~2 test, other SSR markers were detected by linkage analysis.

二、参照本实验室海陆种间遗传连锁图谱第12染色体上的SSR标记,平均每5cM左右选择一个标记进行亲本多态性的筛选,筛选出具有多态性的标记位点进一步扩增随机抽取的210个F_2群体单株的DNA,鉴定每个个体的标记基因型。X~2检测剔除偏分离标记后进行连锁分析,发现Gl_2~e基因位于CIR362与NAU5079之间,进一步参考图谱上位于CIR362与NAU5079之间的标记加密图谱,最终将其定位在CIR362和NAU2251b、NAU3860b、STV033之间。

In the research, one kind of guzmania species named ostara was employed to be as material, and the full-length cDNA plasmid library of floral organ was constructed successfully by using SMART technology. The library had 3×10^6 original titer and more than 1 kb insert fragments. After 5'EST sequencing from 2004 positive clone chosen at random and clustering analyse, 1 758 high-quality sequences and 1 365 unigenes including 175 contigs and 1 195 singlets were obtained. These unigenes had 1 283 valid ORFs. COG analysis showed that those proteins coded by EST sequences were divided into 22 classes. Through blast analysis, full length or part cDNA of some genes controlling flower color, development of floral organs, florescence regulation and other breeding value genes were obtained.

本研究以擎天凤梨属品种Ostara为材料,采用SMART技术成功构建了擎天凤梨花器官的质粒型全长cDNA文库,初始文库滴度为3×10^6,插入片段平均长度大于1kb;随机挑取2004个阳性克隆进行5'EST测序,获得高质量序列1758条,经拼接获得1365条单基因簇,其中跨叠群175个和单条序列1195个;经ORF寻找共获得有效ORF1283条;经COG分析EST序列编码的蛋白质被分为22类;经Blast分析后,获得一批花色、花器官发育、花期调控以及其它育种价值基因(包括cDNA全长或片段)。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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