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常染色质

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There were distinct nucleoli and abundant euchromatin. Little heterochromatin could be found.

细胞核内核仁明显,有大量的常染色质,几乎看不到异染色质。

It could be deduced that the regions of the former were the hot ones for recombination and rich in euchromatin, while those of the latter were the cold ones and rich in heterochromatin.

可以推论,前者应当是重组较热,常染色质较为丰富的区域,而后者则为重组较冷,异染色质较为丰富的区域。

In DAPI banding pattern, the dark region is generally thought to be euchromatin rich and the bright region is heterochromatin rich. It just provided the experimental foundation for the above deduction.

在DAPI带型中,暗区一般被认为是常染色质较丰富的区域,而亮区为异染色质较丰富的区域,这正好为上述的推论提供了实验依据。

Since then, the gene localization has developed from simply being described as at the euchromatin region and heterochromatin region to more complicated nuclear localization. Therefore, the gene regulation attracted more attention now.

因此基因在细胞核内的定位就由原来简单认为的异染色质区和常染色质区发展得更为深入了,基因在细胞核内的定位以及位置的变化与基因表达调控之间的关系也越来越受到重视。

The results indicated that the expression level of cgi-100 decreased in K562 cells treated with matrine. Heterochromatin decreased, euchromatin and the proportion of S phase in K562-cgi-100 cells increased, and cell proliferation enhanced.

结果表明,在苦参碱作用K562细胞后cgi-100基因表达下调;重组K562细胞中cgi-100基因过表达,且常染色质增加,异染色质减少,细胞增殖旺盛,S期细胞增多,增殖速度高于对照组。

Euchromatin stains more intensively than heterochromatin during nuclear division.

常染色质具有转录活性,因而可以进行蛋白质的合成,而异染色质通常不具有转录活性。

In result of electron microscope, cells of contrast group have large nuclear, plenty of euchromatin and microvillus, and organells, such as mitochondrion, Endoplasmic reticulum and Golgi apparatus are less.

电镜观察发现未经LTA作用的对照组细胞核大,常染色质丰富,细胞浆中线粒体、内质网、高尔基体等细胞器少,微绒毛较丰富。

It represents 99.4% of the euchromatic DNA and includes one megabase of heterochromatic sequence within the pericentromeric region of the short and long arm of the chromosome.

其中99.4%是常染色质,在着丝粒周围短臂和长臂区域还有1Mb的异染色质序列。

The signals look like noncontinuous beads on the DNA fiber,which consist of multi-copy and arrange tandemly.Fiber-FISH results with 45S rDNA probe showed that an average signal length is about 3~11μm in many tandem copy sequence(measure number,n=8),so we estimated the size of each copy to be approximately 11~30kb in cotton genome.There are dual signals on each end of the mitosis metaphase chromosomes and pachytene chromosomes hybridized with telomere DNA probe.The signals also look like non-continuous beads on the DNA fiber,the length is about 1~9μm,so we estimated the size is about 4~27kb.The signals almost covered the whole mitosis metaphase chromosomes and pachytene chromosomes of G.arboretum Shixiya 1 hybridized with gDNA probe,the euchromatin zone and heterochromatin zone were identified clearly on pachytene chromosomes,and the signals also look like non-continuous beads.Two BAC clones 150-D-24 and 182-F-9 in DNA BAC library of Pima90 were selected as probe to hybridize with mitosis metaphase chromosomes,pachytene chromosomes and DNA fiber of G.raimondii.

棉花FISH技术系统的应用研究。45S rDNA在亚洲棉石系亚1号中期和粗线期染色体上有两对大的信号,在DNA纤维上信号为非连续的念珠状,多个拷贝串联排列,每个拷贝长度大约在3~11μm,推测每个拷贝的实际长度为11~30kb;端粒序列在亚洲棉石系亚1号中期和粗线期染色体端部都有双点信号,在DNA纤维上信号也为非连续的念珠状,不同染色体上信号长度大约在1~9μm不等,推测实际长度为4~27kb;gDNA信号几乎布满整个亚洲棉石系亚1号中期和粗线期染色体,在粗线期染色体上能明显区分出常染色质区和异染色质区,DNA纤维上的信号均为非连续的念珠状。

Immunoelectron microscopy showed that the distinct ultrastructure of fibroblast and the group, spot gold particle specificity distribution shape. The positive markers of CTGF were mainly located in the RER,while there are expression in RER ribosome, Cf, ECM, desmosome connection, euchromatin,et al.

免疫电镜标记显示成纤维细胞细胞超微结构清晰,金颗粒呈团状、点灶状分布,特异性强,CTGF蛋白阳性标记主要位于粗面内质网,粗面内质网核糖体、胶原纤维、细胞外基质、桥粒连接、常染色质等部位也有表达。

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