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The test-tube plantlet was formed after rooting, through the virus detection on test-tube plantlet, the plant without virus was selected out and the bulblet was obtained through transplanting.

生根后形成试管苗,通过时试管苗的病毒检测,选择不带病毒的植株,移栽得到小鳞茎。

Based on the genome conservative sequence of tabacoo mosaic virus, cucumber mosaic virus and potato X virus, special primers were designed respectively. With the RT-PCR, the target fragments about 313,326 and 392 bp were ampilfied separately from the infected sample, but not health sample.

设计烟草花叶病毒、黄瓜花叶病毒和马铃薯X病毒的特异性引物,RT-PCR检测,从感病叶片中分别扩增出313,326,392 bp的目的片断,而健康叶片中无此扩增带。

The ORF2 gene is the most important protein-code gene for porcine circovirus, and is the main gene differing porcine circovirus typel from porcine circovirus type2 (PCV2). To construct a specific PCR detecting method, we designed a pair of primers to amplify a partial fragment of ORF2 gene from PCV2, referring to fifteen strains complete genome published in Genbank. The PCR product was about 0.67kb in size detected by gelose gel electrophoresis.

猪圆环病毒的开放阅读框2(ORF2)是其主要结构蛋白的编码基因,也是区分猪圆环病毒1型(PCV1)和猪圆环病毒2型(PCV2)的重要基因,本研究参照GenBank已发表的15株PCV2基因序列,设计合成一对引物,对PCV2的ORF2基因进行PCR扩增,产物经琼脂糖凝胶电泳,呈现一条大小约0.67kb的特异条带,经条件筛选,建立了猪病料中PCV2感染的PCR检测方法。

The results showed that CMV concentration in tissue cultured plantlets was decreased greatly compared with the parent plantlet.

以带病毒半夏母株为外植体培养的组培苗,其病毒含量以叶片中的相对较高,在块茎和愈伤组织中病毒相对较低。

Your company's Web site is very beautiful fonts, but there is a top-heavy Home feeling GREEN TEA COMPANY too, easily mistaken for content of bad or virus Web site.

贵公司的网站字体非常漂亮,但是网站首页有一种头重脚轻的感觉,GREEN TEA COMPANY 太大了,容易让人误会为内容不好或是带病毒的网站。

In addition, baculovirus are noninfectious to vertebrates, and their promoters have been shown to be inactive in mammalian cells. Futhermore, the expression level of some recombinant proteins is up to 1000 mg/L. Finally, recombinant proteins are soluble and antigenically, immunogenically and functionally similar to their authentic counterparts. In this article, we used the transfer vector pBlueBac of AcNPV (Autographa californica Nuclear Polyhedrosis Virus) to construct the recombinant baculovirus AcNPV-GM-CSF by in vivo recombinantion.

本文中,我们首先利用苜蓿夜蛾核型多角体病毒带β-Galactosidase 基因标记的非融合蛋白基因转移载体pBlueBac,通过细胞内同源重组的方式将hGM-CSF基因成功地插入病毒AcNPV的基因组中。hGM-CSF基因编码信号肽和完整的氨基酸序列。hGM-CSF基因在感染重组病毒的草地夜蛾培养细胞Sf9中得到表达,感染后的Sf9细胞培养液能刺激人骨髓细胞在体外形成典型的集落,表达水平可达2.7〓CFU/ml。

Intravenous drug users are often brought together through mutual sharing of unsterilized syringes without injecting drugs, drug addiction of a few dozen people even shared a syringe with Cou, if one of them infected with HIV, syringes will be contaminated, such Will enable the virus through blood from a syringe to enter another human body.

静脉吸毒者常常聚集在一起相互共用没有经过消毒的注射器注射毒品,毒瘾上来,几个人甚至十几个人凑在一起共用一副注射器,如果其中一人感染有艾滋病病毒,注射器就会被污染,这样就会使带病毒的血液通过注射器从一个人进入另外一个人体内。

But the most perplexing report involved the single case in France — a wild duck found dead in the suburbs of Lyon — because migratory birds from Asia that carry the virus do not normally travel there at this time of year.

但是最令人困惑的报道是关于法国出现的一起个案的:一只野鸭被发现死在了里昂的郊外,但是从亚洲迁徙的带病毒的候鸟通常在这个季节是不经过这条线路的。

Methods Mousetraps were distributed for the investigation; direct immune fluorescence was applied to detect the virus carriage in rats, 3451 mousetraps were distributed and 886 rats were caught, the rats density was 25.67%; 50 caught rats carried EHFV virus (5.64%) It has been proved that there exists a natural cycle of EHFV virus in Kaifeng County; effective measures should be taken to control the epidemic situation of EHFV.

用布夹法开展鼠密度调查,用直接免疫荧光技术进行鼠肺带毒检查。结果共有效布放鼠夹3451夹次,捕获鼠886只,鼠密度为25.67%。带毒50只,鼠类带毒率为5.64%。捕获鼠种有大家鼠、小家鼠、黑线姬鼠、大仓鼠和小仓鼠。结论证实河南省开封县存在肾综合征出血热病毒的自然循环,应采取有效措施控制流行性出血热疫情。

All of them look like boxcars where there's a long car and there's a little space and there's another one attached behind it.

通过与带病毒的动物部位如毛发、粪便、毛皮接触后可以传染给人,病症为皮肤溃烂损伤

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在,'歼灭战perfuming自己与胡努特,那麼,他来到和SAT 。

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