带氧酶

Protoscoleces of cyst were recovered from the brain of sheep naturally infected with coenurus of Taenia multiceps and total RNA was extracted.T.multiceps thioredoxin peroxidase cDNA fragment was amplified by RT-PCR from the total RNA,ligated into pMD18-T vector and sequenced.

从自然感染的病羊脑内采集多头蚴原头节，提取总RNA，采用RT-PCR技术扩增多头带绦虫硫氧还蛋白过氧化物酶基因片段，PCR产物连接到pMD18-T载体，转化至大肠杆菌DH5α后测序，发现克隆到的TmTPx基因片段开放阅读框为453bp，编码150个氨基酸。

RESULTS: Image analysis of two-dimensional map revealed 72 differentially expressed proteins between MHCC97-H and MHCC97-L cells, and 9 protein peaks (including M2 pyruvate kinase,α-subunit of ATP synthase, heat shock protein 60, Toll-like receptor 9, flavoprotein oxygenase, pro-calreticulin, manganese superoxide dismutase, nm23-H1, and G-protein-coupled receptor kinase 5) were further identified by tryptic digestion, peptide mass fingerprinting and mass spectrometry.

结果： 2D图谱显示共有72个差异蛋白条带，共鉴定出了9个差异蛋白，分别为M2型丙酮酸激酶、ATP合成酶α亚单位、热休克蛋白60、Toll样受体9、含黄素单加氧酶、钙网硬蛋白前体、锰超氧化物岐化酶、nm23-H1、G-蛋白偶连受体激酶5；其中4个蛋白在高转移细胞株MHCC97-H中表达升高， 5个蛋白在低转移细胞株MHCC97-L表达升高。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。