带毒素的

Western blotting results showed that the expressed protein had similar immunogenicity with the natural α-BGT protein purified from the venom of Krait Bungarus spp..

经Western blot分析，在大约8 kDa处出现明显的目的带，与预计蛋白分子量大小一致，说明表达产物与天然α-银环蛇毒素具有相似的免疫原性。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

Work its way through the body politic 那句,可以参看下面这个句子:Normal recourse for one bite, as it had been taught to me, was to let the venom work its way through the body. You can find the above sentence at http://www.fanfiction.net/s/2776851/1/End_Notes.其实work its （poison/venom/a pill, etc. way through one's body还是在使用work one's way through sth.

的基本意思，即（所谓work地穿过或完成sth，只不过当it指毒素或蛇或昆虫的毒液，而through后面又接身体时，含义有时是这毒会经由血液循环等最后在身体里化解（因为稀释或身体自带的解毒能力）或从身体里排出。

Crystal by DTT into the protoxin with an end amino group; activation and derivation of abamectin to form abamectin-COONa with a carboxylic group; final bioconjugation by using amino-carboxylic conjugator EDC to achieve the substance combination of two biotoxins.

晶体进行酶解改造，形成带末端氨基的原毒素；将阿维菌素的羟基进行激活、衍生化，形成带羧基的阿维菌素衍生物（Abamectin-COONa）；再利用氨基-羧基偶联剂进行两种生物毒素的生物耦合。

Specific blockade of extrasynaptic NMDA receptors may effectively prevent neuron loss following stroke and other neuropathological conditions associated with glutamate toxicity.

突触外NMDA受体特定阻闭，可能有效预防中风和其他带谷氨酸毒素之神经疾病后的神经元减少。

In this experiment, a new method was used to purify the toxin protein produced by Verticillium dahliae. The supernatant of fungus culture was frozen and dried with Lyophilizer first, and then dialysed by Dialysis Membranes (MWCO 1000) after dissolved in distilled water. This method can eliminate the salt and sucrose in culture medium and reserve the protein component almost completely. VD-toxin were analysed using sodium dodecyl sulfate polyacrylamide gel analysis. The results indicated that the protein components were very complex, and included glycoprotein within 35.8kDa-83.2kDa. Furthermore, treatments on glycoproteins with high temperature, conA and proteinase alleviated, not abolished, their activities.NO and H_2O_2 production were assayed in two cultivars of cotton cells which were treated with VD-toxin.

本实验首先确定了用冷冻浓缩后透析的方法初步提纯棉花黄萎病菌毒素，该方法能有效去除粗提毒素中的蔗糖和盐离子并能最大程度的保留黄萎病菌分泌的蛋白成分；对毒素蛋白成分鉴定结果表明蛋白条带多，每种蛋白的含量少，小分子量蛋白含量多，糖蛋白染色结果显示，毒素蛋白中分子量在35.8kDa-83.2kDa之间的蛋白大多数为糖蛋白，分子量小于35.8kDa的蛋白多为非糖蛋白；性质鉴定结果显示，毒素中有活性的蛋白成分具有部分可耐高温高压，且毒素蛋白中的蛋白成分和糖基成分都具有致萎活性。

Calibration tool storage cabinet rolling rack w/lights statu go rack rolling rack w/hardhats.tool.rarts metal work station office chairs 7"miter band saw fiberglass backing plates 12"band saw filing cabinets 7 air conditioners 6 humidifiers 2 grinders 2-carts w/testing equipment golf cart 6 utility carts left upper wing right upper wing 4 rolls of final bagging disassembled lay up room lexan 4*8*9/8 3 roof top air ducts preforming core fuselage to wing part non conforming steel/alominum insulation tubes left lower wing right lower wing horizontal upper tail 2 sheets of 1honeycomb core office cubicles cabin door frame template 1 medium size cabinet 2 small cabinets flammable container L.H.boom inboard flap hinge(2) R.H.boom inboard flap hinge(1) L.H.outboard nacelle little fuse CSL mold AFT keelgusset mold LH boom inboard mold RH boom outboard mold LH upper fuel tank mold RH lower fuel tank mold universal safety mats(13) aileron bond/drill jigs metal saw hooses 5-lay up tables safety mat trim kit empty rack office chairs left assembled boom right assembled boom utility carts small file cab bagging material black med.

标定工具储藏柜轧制机架瓦特/灯 statu去机架轧制机架瓦特/ hardhats.tool.rarts 金属工作站办公椅 7 &字带锯玻纤板支持 12 &带锯档案柜 7空调 6加湿器 2磨床 2 ，车瓦/测试设备高尔夫球车 6实用车左上翼右上翼 4卷最后套袋拆卸积储室聚碳酸酯4 * 8 * 9月8日 3天台冷气槽预成形核心机身的一部分，以翼不符合钢铁/ alominum 保温管左下翼右下翼横向上尾 2张1 蜂窝芯办公室隔间舱门框模板 1中型内阁 2小柜易燃集装箱 L.H.boom内侧襟翼铰链（ 2 ） R.H.boom内侧襟翼铰链（ 1 ） L.H.outboard发动机舱小保险丝CSL的模具霉菌毒素keelgusset 黄体生成素臂船模生殖健康繁荣舷外模具激素上油箱模具相对湿度较低的燃料箱模具普遍安全垫（ 13 ）副翼债券/钻夹具金属看到hooses 5 -奠定表安全垫装饰盒空架办公椅离开组装繁荣正确组装繁荣实用工具车小档案驾驶室套袋材料黑色med。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。