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Thirty-seven accessions of wild Dactylis glomerata L. were used in assessment of genetic diversity by means of amplified fragment length polymorphism. Nine primer pairs were selected from 64 preparations and then used to analyze the plant materials.

应用扩增片段长度多态性技术,从64个引物对中筛选出9个,分别对37份鸭茅种质进行扩增,共得到400条清晰条带。

Fingerprints of broiler chickens divergently selected for very low den sity lipoprotein in fat line and lean line of the third generation were studied using inter simple sequence repeated technique. Geneti c polymorphism and genetic similarity coefficient between two groups were analyze d. Ten primers were screened from 60 SSR primers and 62 sites were deprived. Of which 39 bands were polymorphic sites and the frequency was 63 percent.

通过对初步进行血浆极低密度脂蛋白双向选择的三世代高、低脂群肉鸡进行ISSR指纹分析,评定其遗传多态性及两群体间的遗传相似系数。60个引物筛选出10个引物用于检测分析;共扩增出62条谱带,其中39个是多态性片段,多态率为63%。

After the second PCR amplification, sixteen differential bands were screened out, of which eleven were co-expressed in P35 female and P45 male, five were only expressed in female at P45, which might underlie the genetic mechanism of the RA dimorphic development in white-rumped munia.

二次PCR扩增后,进一步筛选出16个差异条带,其中11个为P35雌性和P45雄性共同表达的,5个是P45雌性特异表达的。

Research of ISSR molecular marker The Genetic Diversity of 150 cultivars with typical representation in large-flowered chrysanthemum were investigated using 10 primers with clear bands and steady repetitiveness chosen from 50 ISSR primers.

ISSR分子标记研究从50个ISSR引物中筛选出10个条带清晰、重复性良好的引物,对具有典型代表性的150个大菊品种的遗传多样性进行分析。

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