带条筛

The RAPD system of Gladiolus was optimized. Of the 12 random primers analyzed in this research, 9 primers can show steady and polymorphic amplification profiles.

建立了适用于唐菖蒲RAPD分子标记的反应体系，从12条随机引物中筛选出9条可扩增出多态性条带的引物。

The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphic, and 4~9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cultivars was between 0.17~0.72, average value was 0.39. When all of the polymorphic bands were analysed by cluster analysis, 19 potato cultivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato cultivars is wide.

利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析，分别提取19份马铃薯品种的DNA，进行随机引物多态性扩增，从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究，每条RAPD引物扩增出4~9条带，共获得52条带，其中多态性条带为43条；19份马铃薯品种的遗传距离介于0.17~0.72之间，平均值为0.39，平均遗传距离介于0.31~0.51之间；聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类，聚类结果与系谱分析基本相符，同时也说明克新系列马铃薯的遗传基础有所拓宽。

In screens 20 from 100 10bp along with the machine-drawn to increase the effect to direct the thing well to carry on increases, altogether has 188 bandings,118 are the polymorphism belt, accounts for 62.8%, average each DNA polymorphism belt number which directs the thing to increase is 5.90, has promulgated the sweet potato variety heredity multiplicity; construct the shape chart called UPGMA to reflection variety diverse , will supply to try 31 variety divisions will be 4 broad headings, has promulgated between Hunan sweet potato variety from the molecular level and with the foreign country variety between sibship.

从100个10bp的随机引物中筛选出20个扩增效果较好的引物进行扩增，共产生188条带，其中118条为多态性带，占 62.8%，平均每个引物扩增出的DNA多态性带数为 5.90条，揭示了甘薯品种的遗传多样性；根据扩增结果构建出反映品种多样的UPGMA聚状图，将供试31个品种划分为4大类，从分子水平上揭示了湖南甘薯品种之间及其与外域品种之间的亲缘关系。

Genetic diversity among 7 populations of Trillium tschonoskii were analyzed by using inter SSR and amplified fragment length polymorphism molecular markers. The results were as follows:(1)ISSR: 12 out of the 100 primers showed consistent banding patterns and amplification, a total of 135 bands were produced with an arrange of 250-2500bp, 46 of which (34.07%) were polymorphic.

应用锚定SSR和限制性片段多态对7个延龄草居群的遗传多样性进行了分析，结果如下：从100个引物中筛选出12个ISSR引物，在250-2500bp之间共扩增出的135个条带，其中多态性带为46，占34.07％。

Fourteen wampee cultivars collected from Guangdong and Guangxi were used for RAPD analysis in this study. 16 primers selected from 150 random primers were used to amplify 87 DNA fragments and 57 bands (54.0%) showed polymorphisms.

摘 要：对14个黄皮品种进行 RAPD 分析，从150个随机引物中筛选出16个能在种质间表现出多态性的引物，共扩增出 87 条谱带，其中多态性带47条，多态性比例为54.0%。

Based on the higher alcohols and lactic acid metabolism theory, a method was developed to low higher alcohols capability Saccharomyces cerevisiae strains by screening high lactic acid dehydrogenization enzyme activity in this study.

本研究对Y1110菌株及其出发菌株、突变等株16株不同来源的酵母菌株进行全基因组RAPD分析，建立并优化了酵母RAPD扩增体系和分型系统，从20条随机引物中筛选出了3条具有较好的扩增多态性，共扩增出34条RAPD谱带，多态性为85.3％；获得了稳定清晰的RAPD指纹图谱。

The average percentage of polymorphic bands are 72.06% for ISSR and 66.67% for RAPD respectively. The genetic diversity of ginkgo revealed by ISSR (the average effective number of alleles is 1.8780, the average Nei"s gene diversity is 0.4652, and the average Shannon"s information index is 0.6574) are all higher than those by RAPD (the average effective number of alleles is 1.7842, the average Nei"s gene diversity is 0.4317, and the average Shannon"s information index is 0.6211), while the standard errors of the parameters estimated by ISSR were lower. Therefore, ISSR molecular markers are more suited than RAPD molecular markers when testing the genetic diversity of ginkgo populations and determining the genetic relationship of among populations or among individuals which are much similar hereditarily.

本文应用RAPD和ISSR两种分子标记技术，选取中国5个可能的野生银杏居群共计75个样品，对其遗传多样性进行了研究，得出以下结论：(1)用筛选出的12个RAPD引物和10个ISSR引物进行PCR扩增，分别扩增出65条和68条重复性高、清晰的条带，多态性位点百分率分别66.67%和72.06%，ISSR揭示的银杏遗传多样性(平均有效等位基因数目为1.8780，平均基因多样度为0.4652，平均信息指数为0.6574)高于RAPD所得到的结果(平均有效等位基因数目为1.7842，平均基因多样度为0.4317，平均信息指数为0.6211)，其所估算参数的标准差要低于RAPD所估算出的值，因此，在研究亲缘关系非常近的银杏物种的遗传多样性并试图确定居群间或个体间的遗传关系时，ISSR分子标记技术比RAPD分子标记技术更为合适。

The result showed that: 148 poly morphic hands were detected by use of, 22 RAPD primer and 158 allele were detected by use of 34 SSR primer.

利用筛选出的22条RAPD引物，检测到了148条有多态性的带；利用筛选出的34对SSR引物，检测到158个等位基因。

Five polymorphic primers with good, clear bands were got, screening fromseventy-five ISSR primers. These five pairs of primers were used to amplificaterespectively the genomic DNA of 66 different Pterocarya stenoptera individuals and atotal of 45 bands were amplified with 43 polymorphic bands and percentage ofpolymorphic bands 95.6%.

2从75条ISSR引物中筛选出5条多态性好、谱带清晰的引物，用这5条引物对66个枫杨叶样的基因组DNA进行了PCR扩增，共得到45条带，其中多态性带43条，多态性百分率为95.6％。

In cluster group analysis, the four kinds of color-flowered were divided into 2 styles; at the first, the red color and red-white color were grouped together, then they grouped with purple color into one cluster and the white color was single.

共筛选出12个特异引物，扩增出84条带，具有特异性的谱带为28条，占总带数的33%，在聚类图分析中，把4种花色一串红划分为两大类：首先是红色和红白双色聚为一类，然后和紫色聚在一起，白色单独为一类。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了