带条

In this experiment, a new method was used to purify the toxin protein produced by Verticillium dahliae. The supernatant of fungus culture was frozen and dried with Lyophilizer first, and then dialysed by Dialysis Membranes (MWCO 1000) after dissolved in distilled water. This method can eliminate the salt and sucrose in culture medium and reserve the protein component almost completely. VD-toxin were analysed using sodium dodecyl sulfate polyacrylamide gel analysis. The results indicated that the protein components were very complex, and included glycoprotein within 35.8kDa-83.2kDa. Furthermore, treatments on glycoproteins with high temperature, conA and proteinase alleviated, not abolished, their activities.NO and H_2O_2 production were assayed in two cultivars of cotton cells which were treated with VD-toxin.

本实验首先确定了用冷冻浓缩后透析的方法初步提纯棉花黄萎病菌毒素，该方法能有效去除粗提毒素中的蔗糖和盐离子并能最大程度的保留黄萎病菌分泌的蛋白成分；对毒素蛋白成分鉴定结果表明蛋白条带多，每种蛋白的含量少，小分子量蛋白含量多，糖蛋白染色结果显示，毒素蛋白中分子量在35.8kDa-83.2kDa之间的蛋白大多数为糖蛋白，分子量小于35.8kDa的蛋白多为非糖蛋白；性质鉴定结果显示，毒素中有活性的蛋白成分具有部分可耐高温高压，且毒素蛋白中的蛋白成分和糖基成分都具有致萎活性。

The technology of separation and purification of the interesting protein was studied, centrifuge the sample and get the clear supernatant, remove protein by 50％ ethanol, concentrate by drying with freezing way, remove salt and pigment by dialysing, separation with G-50 and CM-cellulose52 column, concentrate by PEG6000, The purified sample was a single band after separation by SDS-PAGE.

研究了目的阿片肽的分离纯化工艺。发酵混合物经过离心取上清，乙醇沉淀去蛋白，冷冻干燥浓缩，透析除盐脱色，聚乙二醇6000反渗透浓缩，G-50柱分离，CM-cellulose52阳离子交换层析，聚乙二醇6000浓缩等步骤后得到了较纯的目的产物，目的产物在SDS-PAGE上呈现单一条带。

Now he travels light with 4 shirts and one pair of black Dickies pants.

现在他只要出行就只带4件tshirts和1条dickies牌子的裤子。

Gene expression in hippocarrr pus could no longer be detected 30 min after microinjection of Met一or Leu一enkephalin into hippocampus.

基因表达，而海马内甲硫或亮脑啡肽注射30 min后再给予脂多糖，则未检测到IL- l a cDNA的扩增条带。

In vs. 9-10 is a list of sinful behaviors,"… understanding this, that the law is not laid down for the just but for the lawless and disobedient, for the ungodly and sinners, for the unholy and profane, for those who strike their fathers and mothers, for murderers, the sexually immoral, men who practice homosexuality, enslavers, liars, perjurers, and whatever else is contrary to sound doctrine."

在第九至十节，是一条清单、罪恶的行为。「要知道律法本来不是为义人设立的，而是为那些无法无天和放荡不羁的、不敬虔和犯罪的、不圣洁和世俗的、弑父母和杀人的、淫乱的、亲男色的、拐带人口的、说谎话的、发假誓的，以及为其他抵挡纯正教训的人设立的。

The results show that:(1) the earthquakes and hot springs are mostly located in the three main NEN trending strike slip fault zones and associated P,R′ faults;(2)The basic structural patterns in the region of high temperature hot springs or strong earthquake epicentres comprise left lateral en echelon faults and p...

结果表明：1研究区地震和温泉主要集中在 3条 NNE向主走滑断裂带及其伴生的 P、R′断裂带；2高温热泉或强震震中区的基本构造样式为走滑断层左行、左阶雁列带和拉分盆地构造；3陆壳结构分层特征是影响该区地震作用强度的一个重要因素。

Shadowless sword manual is the Massachusetts Institute of Technology successfully developed the world's most advanced technology, a high-pressure injection technology, at 0.01 seconds of time to quickly solve a variety of symptoms of aging nano trace elements, in order to fog-state in the form of fixed-point, quantitative, fixed layer, positioning, in order to fan-shaped form of the direct, accurate tire reaches the skin layer, 20-minute onset, 2 hours effective, and 48 hours markedly effective, non-invasive no side effects, can wipe out wrinkles, Sichuan word pattern, raised eyebrow, raised his eyes, his eyes cured pattern, eyes with a black eye, heal nasolabial fold, modified nose shape, face-lift, facial upgrade, double chin, carved facial contours, 7 days to eliminate fat, slim build remodeling , no recovery of cotton contour sculpture mm accuracy can be controlled, not only to solve the aging problem, had a beautiful face can be more delicate to maintain years.

去 美容美发保健品 IM互动商圈，与商家群聊发现更多商机。共有24条同类&美体器材&信息，请通过分页查看 12 3 4 5 无影刀说明书是美国麻省理工大学研制成功目前世界上最先进的技术，一种利用高压喷射技术，在0.01秒的时间内快速将能解决各种不同衰老症状的纳米微量元素，以雾态的形式定点、定量、定层、定位，以扇状的形直接、准确的到达皮肤中胎层，20分钟起效，2小时见效，48小时显效，无创无副作用，可祛除皱纹、川字纹、提眉、提眼角、祛眼角纹、眼带、黑眼圈、平复鼻唇沟、修饰鼻形、瘦脸、面部提升、双下巴、精雕面部轮廓，7天之内消除脂肪，重塑苗条身材，无恢复期，对棉布轮廓雕塑精准度可控制的毫米之间，不光是解决了衰老问题，可使原本漂亮的脸更加精致，维持之久。

Cosette dressed the wound morning and evening with so divine an air and such angelic happiness at being of use to him, that Jean Valjean felt all his former joy returning, his fears and anxieties dissipating, and he gazed at Cosette, saying: Oh!

就在那次访问的第二天早晨，冉阿让来到楼房里，和平时一样镇静，只是左臂上带着一条大伤口，相当红肿，相当恶毒，象是火烫的伤口，他随便解释了一下。

Cosette dressed the wound morning evening with so divine an air such angelic happiness at being of use to him, that Jean Valjean felt all his former joy returning, his fears anxieties dissipating, he gazed at Cosette, saying: Oh!

就在那次访问的第二天早晨，冉阿让来到楼房里，和平时一样镇静，只是左臂上带着一条大伤口，相当红肿，相当恶毒，象是火烫的伤口，他随便解释了一下。

He cooks a mean dish of fettuccine Alfredo, and he plays baseball and basketball without the aid of his tank.

现在，他自己做Alfredo酱汁宽条面，在不带氧气罩的情况下打棒球，篮球。他还在计划着自己的高中生活。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。