带条

Genetic analysis were carried out to identify powdery mildew and strip rust resistance genes in the F2 of Am6-4 amphiploid and wheat variety Jinan17. Results showed that resistances to powdery mildew and stripe rust were controlled by a single dominant gene respectively. 124 SSR primers from genome D were used for marker analysis, marker Xgwm98 150(150为下标) from the chromosome 6D was found to be linked to the new powdery mildew resistance gene with a linkage distance 20.42 cM; A special DNA band was amplified by primere xgwm33 in resistant stripe rust plants, resistance gene for stripe rust was localized on chromosome 1D, and the genetic distance between resistance gene and marker is 8.0 cM.

利用Am6-4与济南17F2分离群体进行白粉病和条锈病抗性基因的遗传分析结果证明，Am6-4中的抗白粉病和抗条锈病基因均为单显性基因；以124对D基因组SSR引物进行标记分析，引物Xgwm98在抗白粉病DNA池和单株中能扩增出特异标记带，标记与抗白粉病基因间的遗传距离为20.42cM，并将抗白粉病基因定位于6D染色体；引物Xgwm33能在抗条锈病DNA池和单株中扩增出特异标记带，标记与抗条锈病基因间的遗传距离为8.0cM，并将抗条锈病基因定位于1D染色体。

A 1172 bp fragment was detected for Wx-7A gene using dominant marker primer from the Wx-A1 normal wheat, a 593 bp and a 574 bp fragment were detected using codominant marker primer from the Wx-A1α normal and Wx-A1 null wheat, respectively.

STS标记结果中，Wx-7A位点的显性标记引物在野生型中扩增出一条1172bp的特异带，即Wx-A1α基因出现；Wx-7A位点的共显性标记引物在野生型中扩增出一条593bp的特异带，在缺失类型中扩增出一条574bp的特异带。

The genetic relationship of 27 sapodilla cultivars and 4 mamey sapoae cultivars were analyzed by using fluorescent-labeled AFLP markers. A total of 1131 AFLP markers between 75 to 500 base pairs were generated with 9 primer combinations, of which 1096 (96.9%) were polymorphic.

利用荧光AFLP分子标记技术对27个人心果品种及4个蔓妹人心果品种资源的亲缘关系进行研究。9对引物共扩增得到1131条带(75~500 bp)，其中多态性带1096条(96.90%)。28个品种具特征性条带，特征带大小为350~500 bp。

Experiments show that C-B spline curve, uniform B-spline with shape parameter, hyperbolic polynomial uniform B-spline with shape parameter and trigonometric polynomial uniform B-spline with shape parameter can be used to produce some frequently used free form curves in the industrial field when the shape parameter is assigned with some specific values. This process is simpler than producing these free form curves by using controlling vertex.

实验证明，C-B样条曲线、带形状参数的均匀B样条曲线、带形状参数双曲多项式的均匀B样条曲线、带形状参数三角多项式的均匀B样条曲线都可利用形状参数的特定取值表示一些工业领域常用的自由曲线，这比起用控制顶点表示同样的自由曲线更为简单。

80 Individuals of ATA-A F2 plants were used for RAPD molecular mapping of the monoecious gene by bulk segregant analysis. 840 random primers were chosen for polymerase chain reaction amplification between monoecious pool and andromonoecious pool, the results showed that: primer S128 was tightly linked to the monoecious character, and primer S493 was tightly linked to the andromonoecious character.

以甜瓜单性花系ATA-A系与两性花品种YJX-1的杂交F2代的80个单株为材料，利用群分法进行单性花基因有关RAPD分子标记研究，筛选分析了840个10bp随机引物的PCR扩增谱带，结果表明，随机引物S128在1180bp，S493在930bp处产生与性别相关的特异性条带，S128的特异条带与单性花性状相关，S493的特异条带与两性花性状相关。

The characteristics of density bands of Porites coral from Meiji reef in Nansha area are revealed by X-radiography of coral slabs and periodical changes of δ^18 O and δ^13 C in the skeletons.

结合珊瑚骨骼X射线照片和δ^18O、δ^13C的年际变化，对南沙群岛美济礁滨珊瑚骨骼岩心的密度条带进行了分析，发现其密度带变化模式多样，初步可归纳为如下3种类型：(1)宽度相近的高低密度条纹均匀、相间地排列，一对高低密度条带平均宽约4mm；(2)在骨骼主生长轴上由近于均匀分布的细密度条带组成。

The primers and the detection programs have been used to detect the pathogens in the inoculated-diseased cucumber plants .The details are reported as follows: 1. By using the primers ITS1/ITS4, only Fusarium isolates could be amplified to produce a special fragment of 750bp. This product generated 480bp, 200bp and 100bp fragments after digested by restriction enzyme HinfⅠonly from the pathogen of Cucurbits Fusarium Wilt.

引物ITS1/ITS4只在镰孢属菌株上扩增到一条750bp分子片段，其他供试菌株无扩增条带产生，扩增产物经HinfⅠ酶解后，只有瓜类尖镰孢菌产生480bp、200bp和100bp 3条带，说明该PCR-RFLP程序对瓜类尖镰孢菌具有特异性；巢式PCR引物BIK1/BIK4和BIK2/BIK3只有在黄瓜尖镰孢菌菌株上扩增到一条800bp分子片段，其他菌株均无扩增条带产生，说明该巢式PCR程序对黄瓜尖镰孢菌具有特异性。

The typical spot symptoms on fruit would decrease the production and merchandise of tomato in the heavy rain of June and July; therefore, the key period for disease control is after rainy season. Quick identification on pathogen was using PCR. 1476bp band was amplified by primer P3、P4of bacterial speck. By using primer CMM5、CMM6 for bacterial canker, 614bp fragment was amplified. These PCR tests provided important foundation for pathogen identification and disease diagnoses. Using primer RST65 and RST69 for bacterial spot, expected 420bp band was not amplified.

通过聚合酶链式反应对病原进行快速鉴定，证明加工番茄细菌性斑点病用引物P3、P4可以得到一条大小为1476bp的特异性条带，番茄细菌性溃疡用CMM5、CMM6可以扩增出一条大小为614bp的特异性条带，这为病害的快速诊断打下了基础，但利用引物RST65和RST69对番茄细菌性疮痂病进行鉴定，没有得到预期420bp的目标条带，故对细菌性疮痂病的快速检测还有待继续研究。

Among the four characteristic bands,air-cured tobacco including maryland tobacco and Burley tobacco,sun cured tobacco including oriental tobacco and sun-cured tobacco,and flue-cured tobacco and air-cured tobacco were distinguished.

两条晾烟类型的特征标记带、一条晒烟类型的特征标记带，一条烤烟与晾烟特征标记带。这些标记不但可以直接转化成SCAR标记，而且可通过分离群体的遗传分析用于烟草类型间遗传转化的特征带，也可进一步用于测序发掘新基因。

The main results are as below:1 we get 17 primers of amplified and polymorphic bands from 700 10 bp radom primers.

再用这17条引物对39个橡胶树品种进行扩增，得到扩增条带100条，不同引物扩增的DNA带数在4～10条之间，平均每个引物扩增5.88条带。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。