带条

In comparison with the reported corresponding sequences, the putative amino acid sequence shares high homology with assimilatory nitrate reductase of other microalgae, with identity 78% to Dunaliella tertiolecta, 60% to Volvox carteri, 58% to Chlamydomonas reinhardtii, and 50% to Chlorella vulgaris, respectively.

2.3 杜氏盐藻NR基因原核表达载体在大肠杆菌中的表达和功能鉴定(1)pMAL-NR-2组大肠杆菌SDS-PAGE结果显示：在140kDa左右出现一条特异性条带，分子量与MBP-NR融合蛋白相符（BMP+NR=42.5kDa+99.5kDa=142kDa），在IPTG（0.15mmol/L）37℃诱导时，该蛋白量占全菌蛋白的21.06％；对照组无此特异带。

Results: Based on the study mentioned above, about 323 bp positive band were amplified under the anneal temperature of 65℃ by total volume of 25 25ul PCR reaction. Sequencing results prove the positive band were fragments of Cytb genes from both Cervus elaphus Linnaeus and Cervus nippon Temminck.

结果：在方法学考察的基础上，在25ul PCR反应体系，退火温度为65℃的条件下，能准确扩增出约323bp 大小的阳性DNA条带，经测序验证结果表明，系正品鹿茸原动物的Cytb基因序列片段，而伪品鹿茸未扩增出条带。

There are experimental results as follows:(1) Eriophorum comosum Nees shows strong cold resistance in plasmalemma permeability research. The lethal temperature of Eriophorum comosum Nees is the lowest LT_(50 in comparison with another two plants and is lower than -11.5℃ in August 2005 and December 2005; Soluble protein content of each plant is rising before or after cold hardiness. By SDS-PAGE, Protein band is analysed. Band quantity of Eriophorum comosum Nees is the most and its band color is darkest.(2) Soluble sugar content of three plants after cold hardiness are higher than before cold hardiness, moreover soluble sugar content after cold hardiness shows a decline and there is a narrow range in Eriophorum comosum Nees; Free Proline accumulation is changed by cold stress, and cold resistance is relative to free Proline accumulation; chlorophyll content of both three plants, including chlorophyll a, chlorophyll b and chlorophyll a/b, show a decline tendency along with the descent of temperature. Cynodon dactylon is highly sensitive to cold stress.(3) The soil POD and urease activities' absolute value of Erioophorum comosum Nees and Pogonatherum panideumHack were more than that of Cynodon dactylon before or after low temperature stress, especially POD activities.

实验结果表明：(1)在膜透性的研究中，丛毛羊胡子草抗寒性最强，采用电导率法测得半致死温度LT_(50最低，丛毛羊胡子草抗寒锻炼前后半致死温度LT_(50均小于-11.5℃；在可溶性蛋白含量研究中，抗寒锻炼前后三种植物的可溶性蛋白含量均增加，同时采用SDS-PAGE法，通过对蛋白条带分析，以丛毛羊胡子草条带数最多且着色深；(2)在可溶性糖的研究中，三种植物经抗寒锻炼后叶片可溶性糖含量都比抗寒锻炼前高，且经过抗寒锻炼后三种植物可溶性糖均呈下降趋势，丛毛羊胡子草可溶性糖含量下降较小；从游离脯氨酸含量可知，低温的胁迫影响了植物叶片游离脯氨酸的含量变化，植物的抗寒性与游离脯氨酸有关；低温胁迫下三种植物叶绿素含量随着温度的下降，叶绿素含量、叶绿素a、叶绿素b和叶绿素a／b均呈下降趋势，狗牙根对低温胁迫最为敏感；(3)低温锻炼前后，丛毛羊胡子草和金发草的土壤过氧化物酶与脲酶活性的绝对值均大于狗牙根的，特别是过氧化物酶，说明丛毛羊胡子草和金发草的酶活性大于狗牙根的。

Choose 37 ACGM primers to PCR amplification from 55 gramineae germ plasm, 569 bands are obtained, and all of the bands are polymorphism.

利用37对ACGM引物对55份禾本科种质进行PCR扩增，共扩增出569条谱带，皆为多态性条带。

The parameter of beam positions at HLS 200 MeV LINAC is very important to injection efficiency.A new strip-line beam position monitor system was designed recently,which is not interceptive to beam,qualified in precision and easy to digitalize the results.

合肥光源(Hefei Light Source，HLS)200 MeV直线加速器的束流横向位置是一个重要的运行参数，直接决定注入的效率，为此新开发了一种非拦截型、高精度、易于将测量结果数字化的条带电极束流位置测量系统(beam position monitor， BPM)，该系统由条带电极和信号处理系统组成。

Characteristic bands of the resistance gene to powdery mildew Pm21 and Pml3 were found, which could be used in the identification of the disease resistance genes and near isogenic lines.

5发现抗白粉病基因Pm21供体亲本及其近等基因系的特征条带，以及Pm13供体亲本及其近等基因系的特征条带，可用于抗白粉病基因及近等基因系的鉴定。

The genomic DNA of 42 accessions of leucaena were amplified by using 3 pairs of AFLP primers: E-ACC/M-CTC, E-ACG/M-CTT, E-ACC/M-CAA, and 125 clear bands were produced, of which 108 were polymorphic.

其中17条带为所有材料共有，占14.29%，108条为多态性带，平均多态性百分比为85.71%，从DNA分子水平显示出银合欢具有丰富的遗传多样性。

A total of 13 bands were obtained and 6 of them (A1, A3, A4, A5, A9, A10) were sequenced. The sequences are similar to Leuconostoc mesenteroides (GenBank Access No.AY453065), some uncultured bacteria (AJ318147, AF227834, AJ576427), Ethanologenbacterium (AY434722), Clostridiaceae (AB084627), etc.

共得到13个可辨晰的SSCP条带，对其中的6条带(A1，A3，A4，A5，A9，A10)进行了测序分析，分别同嗜柠檬酸明串球菌(GenBank登录号：AY453065，下同)、未培养细菌(AJ318147，AF227834，AJ576427)、产乙醇杆菌(AY434722)、梭杆菌(AB084627)等相似性较大。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Two polymorphic loci, Aat-1 and Aat-2, and one monomorphic locus, Aat-3, were observed in C. hytivusparents and their progenies. At Aat-1, in C. sativus fast-migrating anodic band coded for Aat-1 (2), however, in C.

在Aat-1，新种父本表现为一条阳极快带，为等位基因Aat-1(2)所编码；新种母本则表现为一条阴极慢带，为等位基因Aat-1(1)所编码。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。