带卵的

We detected the frozen-thawed sperm of 36 Simmental,23 Charolais and 12 Limousin,the results showed that the frozen-thawed sperm of these three breeds could penetrate the zona-free ova of hamster,and formed male pronucleuses.

对36头西门塔尔、23头夏洛来和12头利木赞牛冷冻精子的实验结果表明：三个品种的牛冻精均能穿入去透明带仓鼠卵，并形成发育良好的雄原核。

The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.

本试验以波尔山羊耳成纤维细胞为供体，分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植，构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应，并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法，探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响，旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。

Results One 446 bp PCR product was observed by agarose gel electrophoresis for all three kinds of templates. The PCR sensitivity by Chelex-100 extracted DNA reached for detection of a specimen containing only 1/2 oocyst.

结果3种方法制备的微小隐孢子虫卵囊模板用于PCR检测均获得1条446 bp条带，Chelex-100提取的DNA用于PCR检测的敏感性至少达0.5个卵囊。

The oocytes and early embryos treated by other three methods were out of sharp and the structure of nucleolus、ZP and polar body in different stages were damaged.

其它3种方案处理后卵母细胞的形态变形，各发育时期的核仁、透明带、极体等结构受到不同程度的破坏。

The variation to vitellin polypeptide was studied by SDS-PAGE during embryogenic and postembryonic development to Chrysopa septempunctata. The results indicated that vitellin was degradated constantly during embryonic development, and the bands of vitellin couldn't be detected in the 4th day of ovum development.

用SDS-PAGE研究了大草蛉胚胎发育和胚后发育过程中卵黄蛋白多肽的变化动态，结果表明，卵黄蛋白在胚胎发育过程中被不断降解，在卵发育的第4天已经基本上看不到卵黄蛋白的条带。

Increased motility, zona binding and oolemma fusion capacities are also acquired within the epididymis and are necessary for those cells that finally arrive at the site of fertilization.

精子在附睾中同时提高了运动力、透明带的结合能力以及与卵膜融合的能力，而这些能力是精子到达授精位置所必需的。

The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构胚是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的，可在体外发育至孵化囊胚阶段，我们应用一种简便、快速的异种重构胚的鉴定方法，即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构胚的核遗传物质来源于大熊猫供核体细胞（后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性）。

Leaves lanceolate to ovate-lanceolate, short petiolate to subsessile; corolla usually creamy white to reddish, purple spotted.

叶披针形到卵状披针形，对近无柄的短叶柄；通常的花冠乳白色的到带红色，紫色斑点。

Both the proteins components contained in haemolymph, fat body and ovary and the kenetics of the vitellogenin in haemolymph and ovary were analyzed by SDS—polyacrylamid electrophoresis, Ouchterlony plate tests and Rocket—immuno—electrophoresis. The results showed that the vitellogenesis initiated in adult stage and the Vg in haemolymph was detected firstly at 48h after eclosion. The titer of Vg in haemolymph raised rapidly in following 3 days and almost at the same time the vitellin appeared in oocytes. It was taken about 4—7 days after eclosion for the first eggs to grow up to the matured statics and to be laid out.

SDS-PAGE，双向免疫扩散和RIE分析粘虫生殖周期中血淋巴，脂肪体和卵巢中蛋白质谱带以及Vg滴度的变化，结果表明，Vg发生始于成虫期，Vg在血淋巴中的首现时间在羽化的24小时以后，以后连续三天血淋巴中的Vg滴度迅速上升，与此同时，开始沉积卵黄，卵母细胞逐渐发育长大，到成熟排出体外时大约历时4-7天。

As it swims with its huge 4-foot-wide mouth open, prey like these dog snapper eggs gets trapped in a sieve or rakers

在它游动的同时，它四英尺宽的大嘴会完全张开，像九带笛鲷卵这样的猎物会被滤筛或滤耙拦住

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。