带卵的

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以预先吸有细胞核或细胞的注射针在固定于持卵针上的卵母细胞透明带上穿刺两个孔，然后一边缓慢地将注射针回拔至卵周隙中，一边逐渐增加持卵针中的负压，直至极体与目标核质被完整吸入持卵针中而完成去核，最后在不拔出注射针的情况下直接注射细胞核或完整细胞进而完成重构胚的构建。

The porine is the polyembryony animal, many obtains the ovocyte from the porine ovary although but the maturity quite to be low; Because the ROSI technology is does not have to grow the mature sole round sperm cell to pour into directly completely in the ovicell nature, jumped over the spermatozoon in to pass through the physiology and the biochemistry, if the ovicell nature mature or the activation degree were insufficient, added the round immature sperm cell maturity quite inferior reason, very possibly Causes the ROSI micro fertilization defeat.

猪是多胎动物，从猪卵巢上获得的卵母细胞数量虽然较多，但成熟度比较低；由于ROSI技术是将没有完全发育成熟的单一圆形精细胞直接注入卵胞质内，跳越了精子在穿过透明带和卵质膜等过程中所发生的生理和生化反应；如果卵胞质成熟或活化程度不够，加之圆形未成熟精细胞成熟度比较差等原因，很可能造成ROSI受精的卵母细胞内部激活因子蓄积减少和生成不足，引起精核解聚困难、精圆核不能形成、第二极体不能排出、卵母细胞孤雌发育率增加等，使ROSI显微受精失败。

Basal leaves often withering early; petiole 1.2--1.5 cm. Stem leaves usually in whorls of 4, sessile or petiole to 2.2 cm, densely villous; leaf blade long ovate or ovate-oblong, 2--5 X 1.1--2.2 cm, abaxially whitish scurfy, adaxially sparsely pubescent, pinnatifid; segments triangular-ovate to long ovate, dentate.

基生叶通常早枯萎；叶柄1.2-1.5 厘米茎生叶通常在4，无梗或叶柄在2.2厘米，密被长柔毛轮生方面；叶片长卵形或卵形长圆形，2-5 X 1.1-2.2厘米，背面带白色具鳞屑，正面疏生短柔毛，羽状半裂；裂片三角状卵形的到长卵形，具牙齿。

The former shifts equatorward when AE increases, just as the auroral oval does; the latter is divided into two sections: the midnight section shifts poleward when AE increases, while the morning section shifts equatorward.

在亚暴膨胀相，随着AE指数增大，整个极光卵向赤道扩展，而极光电集流带却表现出分段差异的特点：下午—黄昏东向电集流带向低纬移动，晨侧西向电集流带也向赤道移动，而子夜—凌晨西向电集流带则向极移动。

Methods A C57BL/6j mouse cumulus cell nucleus 10-12 mm in diameter was inserted into the perivitelline space of an enucleated oocyte.

方法将直径10~12mm的C57BL/6j小鼠卵丘细胞核注射到去核卵母细胞透明带下，构建供体核-卵母细胞复合体。

The sperm subzonal insemination on mice denudedoocytes. This part of paper use SUZI method to investigate the effects of zona pellucidaon denuded oocytesfertilization.

二、精子注入去除卵丘细胞的小鼠卵母细胞的体外受精利用透明带下精子注射技术，研究透明带对受精的影响。

Under the transmission election microscope, we had observed that the state of oocytes arid the cumulus cells around them was tightness, and there were plenty of long and thin microvilli playing the role of connection in the enwrapping. The shape of cumulus cells nucleus was ovate, and the longer and shorter diameters of the cumulus cells were 4.250±0.042μm and 2.750±0.07 μm respectively. The zona pellucida was well proportioned and tightly linking with oocyte. There were great deals of multi-crista mitochondria, lipid droplet and vacuolus. The mitochondria longer and shorter diameters were 0.600±0.106μm and 0.490±0.117μm. Granular nucleoplasma distributed very well in the cell nucleus.

电镜下，卵母细胞外的卵丘细胞紧紧包裹卵母细胞，且与卵母细胞之间有大量细长的微绒毛相联系；卵丘细胞核呈卵圆形，其长径为4250±0.042μm，短径为2.750±0.071μm；透明带均匀，与卵母细胞结合紧密；在皮质区集中分布大量的多峰型线粗体、脂滴和空泡，线拉体长径为0.600±0.106μm，短径为0.490±0.117μm；细胞核中颗粒状的核质分布非常均匀。

Besides these, different kinds of chromosomes were discriminated exactly by initial analyzing the bandings of Trachinotus ovatus, which made karyotype analysis much more standard.

在此基础之上，我们还对卵形鲳鲹进行了初步的带型分析，发现以染色体带纹为标记，可以更准确的识别染色体，确定同源染色体，使对卵形鲳鲹的核型分析标准化。

Cell-cycle synchronization between the donor cell and recipient oocyte determines the embryo development in nuclear transfer. In the present study, we microinjected primary spermatocyte into the perivetelline space of oocyte. 37% pairs fused after electric stimuli and the resulting oocytes were culture for 2 h in MEM with or without CB.

在本研究中，我们将小鼠的初级精母细胞显微注入MI卵母细胞的透明带下，经直流电脉冲作用后有37％的初级精母细胞融入卵母细胞，然后将融合的卵母细胞分成两组在MEM和含有CB的MEM培养液中分别培养，2小时后将在CB培养液中培养的卵母细胞转入正常培养液中。

However, when two maturing oocytes fused and two spindle will form in the big cell, the chromosomes will not intermingle. In the present study, we removed GV from one oocyte and transfer to the perivetelline space of another GV oocyte. After fusion the resulting oocytes which contained two GVs were cultured further in MEM.

在本实验中，我们利用小鼠GV期卵母细胞将一枚卵母细胞的生发泡取出后移至另一未经去核处理的GV期卵母细胞透明带下，经三次电脉冲作用后将融合的含有两个GV的卵母细胞放入MEM成熟培养液中培养，在培养成熟的不同阶段分别收集卵母细胞进行免疫荧光染色观察微管及核的变化。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。