布鲁氏菌

In this report, we constructed a new suicide plasmid to construct Brucella mutant.

本研究构建了一个可用于布鲁氏菌突变株构建的自杀质粒。

Development of a marker of the vaccine and find a suitable brucella diagnostic antigen has been the peoples goals.

寻找合适的布鲁氏菌诊断性抗原和研制具有标记性的疫苗一直是人们追求的目标。

The method can also be used for the examination of Brucella DNA in serum or other aborted pathologic materials.

本方法同样可用于血液或其他流产病料中布鲁氏菌DNA的检测。

Brucella outer membrane protein as the immunogenic and protective effect antigen has been well known to the world.

布鲁氏菌外膜蛋白位于细菌细胞膜的最外层，因其具有免疫原性和免疫保护作用一直备受注目。

Objective To identify the major outer membrane proteins of Brucella Melitensis with proteome analysis.

目的：通过蛋白质组技术阐明羊布鲁氏菌外膜蛋白的主要组成。

A high specific and sensitive sandwich ELISA for human brucella antigen detection has been successfully developped.

建立了高特异性、敏感性的检测人布鲁氏菌抗原的ELISA方法。

Using this plasmid, we constructed replacement mutant of Brucella omp25 gene.

利用该质粒，我们构建了布鲁氏菌外膜蛋白Omp25基因的突变株。

The successfully constructed new suicide plasmid pUC19-SacB can be used to construct a Brucella unmarked mutant.

构建了一个新的自杀质粒pUC19-SacB，该质粒能用于布鲁氏菌无痕缺失突变株的构建。

ResultsUsing this method, we successfully cloned the 11kb virB operon of Brucella and constructed complementary plasmid.

我们利用该方法克隆了布鲁氏菌中长度为11kb的virB操纵子，并构建了互补质粒。

It is apparent that the VirB8-PCR assay appears to be specific and sensitive and can be used for the detection of the virulence factor of Brucella as well as the surveillance of brucellosis.

VirB8-PCR方法特异、敏感，能用于布鲁氏菌病的监测和致病力因子的检测。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。