布鲁氏菌

Objective To clone the BP26 gene of B. abortus, highly express in K. coli and purify the expressed product. Methods Extract the genomic DNA of B.

目的 克隆布鲁氏菌BP26基因，并在大肠杆菌中高效表达及纯化BP26蛋白。

Key words: Brucella abortus;omp25 gene;cloning;prokaryotic expression

流产布鲁氏菌；omp25基因；克隆；原核表达

The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3. Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells, and the expressed OMP 17.3 was detected by Western-blotting.

采用PCR方法扩增了布鲁氏菌17.3ku外膜蛋白编码基因，并将该基因克隆至真核表达载体pcDNA3.1中，成功构建了真核表达质粒pcDNA3.1-omp17.3.pcDNA3.1-omp17.3转染COS-7细胞后，通过Western-blotting检测到了17.3ku蛋白的瞬时表达。

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

The most well-known pasteurization process is associated with fluid milk, where the minimum process is that necessary to eliminate human health concerns associated with Brucellosis andor Tuberculosis.

巴氏杀菌过程最经常应用在液态乳上，是除去乳中与人体健康相关的布鲁氏菌和或结核杆菌而采用的最低强度的处理。

This is the web page of Brucella: Molecular and Cellular Biology.

这是布鲁氏菌：分子与细胞生物学的网页。

Development of a new suicide plasmid and its application to Brucella mutant construction.

布鲁氏菌自杀载体的改建及其在突变株构建中的应用。

Objective To develop a high specific and sensitive ELISA for human brucella antigen detection.

目的 建立一种高特异性、敏感性的 ELISA 方法检测人布鲁氏菌抗原。

Any of various aerobic, short, rod-shaped bacteria of the genus ''.

布鲁氏菌布鲁氏菌属的好氧性细菌，短棍状，是人类的和家畜的病源

Experimental animals exhibited specific antibodies that were detectable 15 days after the first injection and increased steadily, the IgG2a-specific antibody titers of DNA groups were higher than IgG1-specific antibody titers, and the recombinant protein groups were contrary. The DNA vaccines elicited a T-cell-proliferative responses as well as IFN-γ production upon restimulation with the specific antigens using ELISPOT assay; the ratio of CD4+/CD8+ were lower than that of the control mice.

本研究成功构建了布鲁氏菌基因工程疫苗，在国内尚属首次，利用流式细胞仪的分型技术和ELISPOT法评价布鲁氏菌疫苗的细胞免疫指标将促进我国兽用疫苗及相关产品的实验室质控标准方法的建立和发展，为他们的临床应用奠定基础。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。