布鲁氏菌

Additionally,this study developed three species-spcific real-time fluorescence quantitative PCR-based assays for rapidly, specificity, sensitivity,detecting Brucella abortus, Brucella melitensis, Brucella suis.

在建立布鲁氏菌属荧光定量PCR检测方法的基础上，根据布鲁氏菌基因组多拷贝插入元件IS711及其下游多态位点，设计了检测牛、羊、猪种布鲁氏菌的引物和牛、羊、猪种特异性荧光探针，优化了牛、羊、猪种布鲁氏菌荧光定量PCR反应体系和条件，建立了牛、羊、猪种布鲁氏菌荧光定量PCR检测方法，组装了牛、羊、猪种布鲁氏菌检测试剂盒，该方法能特异检测上述3个种的布鲁氏菌。

Every year Brucella caused about 500,000 cases of Brucellosis in mankind in the world.

据世界卫生组织统计表明，全世界每年出现50万例人布鲁氏菌病，每年因布鲁氏菌病造成的经济损失近30亿美元。

Any of various aerobic, short , rod-shaped bacteria of the genus Brucella that are pathogenic to human beings and domestic animals.

布鲁氏菌布鲁氏菌属的好氧性细菌，短棍状，是人类的和家畜的病源

From sample treatment to data analysis of the detection, 30 minutes is enough to complete the whole detection process, providing a new on site detection method for Brucella.

整个检测过程，从样品处理到数据分析，能够在30分钟内完成，从而实现了布鲁氏菌的现场快速检测，为布鲁氏菌的现场快速检测提供了一种新的方法。

Brucellosis is a zoonotic disease that was caused by brucella spp..It is severe affectting humans health and the development of animal husbandry.

布鲁氏菌病是由布鲁氏菌引起的一种人畜共患病，给畜牧业的发展和人类的健康带来了很大危害。

Objective To study the method to identify Brucella suis biovar 1 wild strain and S2 vaccine strain.

目的 寻找猪种布鲁氏菌生物1型野毒株（1330S）和猪种布鲁氏菌2号菌株（S2）的鉴别诊断方法。

It was popular in some districts and even it pervaded seriously in a few ones. 101 positive cows were detected in total and 2 strains of type 3 Brucella were isolated.

对101份布鲁氏菌病血清学检测阳性奶牛的牛乳进行细菌分离培养，结果分离出2株牛种生物3型布鲁氏菌。

Using pUC19-SacB, we successfully constructed an unmarked deletion mutant of the VirB operon, showing that this plasmid can be efficiently used to modify the Brucella chromosome.

我们利用该质粒，成功的构建了布鲁氏菌Ⅳ型分泌系统的突变株，改建的载体能高效的应用于布鲁氏菌染色体的精确修饰。

Besides its simpliness and quickness, the method also has characters of specificity and sensitivity. It has a great importance to detect and diagnose Brucellosis by Polymerase Chain Reaction.

该奶牛布鲁氏菌病病原PCR快速检测方法的建立，对奶牛布鲁氏菌病的检疫及快速诊断具有十分重要的意义。

The second grade class infectious disease is refers: Toxic hepatitis,bacterium and amoeba dysentery, typhus and 副伤寒, AIDS, gonorrhea,syphilis, polio, measles, whooping cough, diphtheria, epidemic脑脊髓膜 inflammation, scarlet fever, epidemic hemorrhage heat,rabies, hook end spirochetosis,布鲁氏菌 sickness, anthrax,epidemic and local macula typhus, epidemic encephalitis b, blackfever, malaria, Dengue fever, high pathogenic birds and beasts flu andinfection atypical pneumonia.

乙类传染病是指：病毒性肝炎、细菌性和阿米巴性痢疾、伤寒和副伤寒、艾滋病、淋病、梅毒、脊髓灰质炎、麻疹、百日咳、白喉、流行性脑脊髓膜炎、猩红热、流行性出血热、狂犬病、钩端螺旋体病、布鲁氏菌病、炭疽、流行性和地方性斑疹伤寒、流行性乙型脑炎、黑热病、疟疾、登革热、高致病性禽流感和传染性非典型肺炎。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。