已受精的

Part one: Previous results indicated that the abnormal development of haploid goldfish embryos is due to the expression obstruction of some proteins, which are correlative with eyes development, nerve development, development regulation, cell differentiation and maintenance, arthromere formation and signal transduction.

第一部分金鱼雌核发育过程中胚胎蛋白质组比较研究前期已发表的工作表明在金鱼雌核发育眼睛形成期(受精后26个小时)单倍体上一些与眼睛发育、神经发育、发育调控等相关的重要蛋白质未能被选择表达或表达受阻，从而导致单倍体发育畸形。

It has been legal in Britain since 2000 to fertilise eggs that have been frozen and thawed,and four babies have been born so far.

据英国的法律自2000年以来已受精冷冻和解冻鸡蛋，四个婴儿已经出生了，所以far。

Days after the fertilized egg, who is scheduled to shape, that is.

卵受精后14天，人的形状已定，即是人。

Next, they "planted" fertilized oyster eggs, which

接 着，他们"栽种"已受精的牡蛎卵。

That could mean munching on live sea horses or hard-boiled fertilized duck eggs — though steering clear of adulterated chicken breasts.

那可意味着大声咀嚼生海马或是煮过熟了的已受精鸭蛋，虽然避开了掺入次级品的鸡胸肉。

Ooplasmic transfer increases the fertilizability of the aged oocytes and the early embryonic viability, it has led to the birth of health babies, and become a hotspot in the realm of human assisted reproduction biotechnology and animal clone.

卵胞质转移技术能有效改善老龄卵母细胞的受精能力和早期胚胎的发育能力，在人类已有健康后代出生，它已成为人类辅助生殖生物技术和动物克隆研究的新热点。

So they set up well-equipped hatcheries and went to work.

接 着，他们&栽种&已受精的牡蛎卵。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

Pig oocytes cultured in vitro for some time were inseminated by frozen–thawed ejaculated sperm. At specified times after insemination, sperm penetration, cell cycle progression and mitogen-activated protein kinase phosphorylation were evaluated. It was shown that:(1) oocytes at various maturational stages could be penetrated by sperm;(2) sperm penetration did not affect meiotic cell cycle progression;(3) sperm penetration of germinal vesicle oocytes and maturing oocytes did not alter MAPK phosphorylation; and (4) when premetaphase I and metaphase I oocytes, in which MAPK was activated, were fertilised, no evident MAPK dephosphorylation was detected as in metaphase II oocytes.

不同成熟阶段的猪卵与精子融合后体外培养发现：(1)不同成熟阶段的猪卵母细胞都可被精子穿透；(2)精子穿透不影响减数分裂细胞周期进程；(3)精子穿透GV期卵母细胞和正在成熟的卵母细胞不改变MAPK磷酸化：(4)已受精的前中期I和中期I卵母细胞的MAPK有活性，MAPK不像MII卵母细胞那样受精后发生磷酸化。

Researchers have successfully produced a group of them using artificial insemination.

研究人员使用人工受精的方法已成功繁衍了一群树袋熊。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。